Anti-Strep-Tag II Monoclonal Antibody(8C12) Review

Strep-tag II antibody detects amino acid sequence specific for Streptavidin. Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-

terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered (and re-named Strep-tag II) to allow it to also placed at the N-terminus of recombinant proteins. Anti-Strep-tag II antibodies are ideal for investigators involved in GFP and Epitopes research.

Anti-Strep-Tag II Monoclonal Antibody(8C12) was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody has been routinely tested with WB applications. And Abbkine suggested starting dilutions are as follows: WB 1:2000-5000.

Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. It is responsible to say that Anti-Strep-Tag II Monoclonal Antibody(8C12) has a reliable quality.