Affinity of Protein A, Protein G and Protein A/G

Protein A, Protein G and Protein A/G are often used in antibody purification and other applications such as immunoprecipitation (IP) and chromatin immunoprecipitation(ChIP). Their binding abilities to antibodies of different subtypes and species are varied. How to choose correct types of protein to purify your sample? Here, we list the antibody binding properties of Protein A, Protein G and Protein A/G.

Note: +++ strong binding; ++ medium binding; + weak binding; - no binding

Species	Antibody subtype	Protein A	Protein G	Protein A/G
Human	Total IgG	+++	+++	+++
	IgG1	+++	+++	+++
	IgG2	+++	+++	+++
	IgG3	+	+++	+++
	IgG4	+++	+++	+++
	IgM	+	-	+
	IgD	-	-	-
	IgA	+	-	+
Mouse	Total IgG	+++	+++	+++
	IgG2a	+++	+++	+++
	IgG2b	++	++	++
	IgG3	+	++	++
	IgM	-	-	-
Rat	Total IgG	+	++	++
	IgG1	+	++	++
	IgG2a	-	+++	+++
	IgG2b	-	+	+
	IgG2c	+++	+++	+++
Rabbit	Total IgG	+++	+++	+++
Goat	Total IgG	+	+++	+++
Sheep	Total IgG	+	+++	+++
Guinea pig	Total IgG	+++	+	+++
Hamster	Total IgG	++	++	++
Donkey	Total IgG	++	+++	+++
Horse	Total IgG	+	+++	+++
Cow	Total IgG	+	+++	+++
Pig	Total IgG	+++	+	+++
Dog	Total IgG	+++	+	+++
Cat	Total IgG	+++	+	+++
Chicken	Total IgY	-	-	-