Affinity of Protein A, Protein G and Protein A/G

Protein A, Protein G and Protein A/G are often used in antibody purification and other applications such as immunoprecipitation (IP) and chromatin immunoprecipitation(ChIP). Their binding abilities to antibodies of different subtypes and species are varied. How to choose correct types of protein to purify your sample? Here, we list the antibody binding properties of Protein A, Protein G and Protein A/G.

Note: +++ strong binding; ++ medium binding; + weak binding; - no binding

Species	Antibody subtype	Protein A	Protein G	Protein A/G
Human	Total IgG	+++	+++	+++
	IgG1	+++	+++	+++
	IgG2	+++	+++	+++
	IgG3	+	+++	+++
	IgG4	+++	+++	+++
	IgM	+	-	+
	IgD	-	-	-
	IgA	+	-	+
Mouse	Total IgG	+++	+++	+++
	IgG2a	+++	+++	+++
	IgG2b	++	++	++
	IgG3	+	++	++
	IgM	-	-	-
Rat	Total IgG	+	++	++
	IgG1	+	++	++
	IgG2a	-	+++	+++
	IgG2b	-	+	+
	IgG2c	+++	+++	+++
Rabbit	Total IgG	+++	+++	+++
Goat	Total IgG	+	+++	+++
Sheep	Total IgG	+	+++	+++
Guinea pig	Total IgG	+++	+	+++
Hamster	Total IgG	++	++	++
Donkey	Total IgG	++	+++	+++
Horse	Total IgG	+	+++	+++
Cow	Total IgG	+	+++	+++
Pig	Total IgG	+++	+	+++
Dog	Total IgG	+++	+	+++
Cat	Total IgG	+++	+	+++
Chicken	Total IgY	-	-	-

Anti-mCherry Tag Mouse Monoclonal Antibody (9D3) Review

mCherry is a fluorophore (a fluorescent protein) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cell components. mCherry and the majority of red fluorescent proteins derive from a protein isolated from Discosoma sp., while other fluorescent proteins in the green range are often variants of GFP from Aequorea victoria. mCherry is sometimes preferred to other fluorophores due to its colour, as well as its photostability compared to other monomeric fluorophores.

Anti-mCherry Tag Mouse Monoclonal Antibody (9D3) was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody has been routinely tested with WB application. And Abbkine suggested starting dilution is as follows: WB 1:5000.

The mCherry Tag Antibody can identify recombinant protein marked by mCherry that have alread been expressed, including those in the amino acid and the carboxyl terminal. Anti-mCherry Tag Mouse Monoclonal Antibody (9D3) worked very well. I am satisfied with the results of the experiment.

Easy use PurKine™ Biotin-Tag Protein Purification Kit (Streptavidin)

Streptavidin is a 60kDa protein from Streptomycetes avidinii. The protein is a tetramer having four biotin-binding sites. Unlike avidin, streptvidin has a low isoelectric point (pI=5) and no carbohydrate groups, resulting in low nonspecific binding.

PurKine™ Biotin-Tag Protein Purification Kit (Streptavidin) is based on innovative high-capacity matrix for convenient single-step purifications of biotin and biotinylated substances. Biotinylated antibodies, proteins, peptides, nucleic acids and other molecules or interaction complexes can be captured, immunoprecipitated, removed or purified from samples using this streptavidin resin. The Resin consists of 90μm beads of cross-linked 6% agarose, to which Streptavidin has been coupled. Tests confirm that performance equals or exceeds popular Streptavidin resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.

Per mL of streptavidin resin can capture 1 to 3 mg of biotinylated BSA or more than 120 nmol Biotin. PurKine™ Biotin-Tag Protein Purification Kit (Streptavidin) contains PurKine™ Biotin-Tag Streptavidin Packed Column 6FF and buffers.There is no need for users to pack resin or prepare buffers themselves which can save precious time. In addition, because of the highly crosslinked resin, the tolerate linear flow rates of the kit is up to 500 cm/hour. It's totally ok for scaling up.

Anti-MBP Tag Mouse Monoclonal Antibody (9Y5) Review

MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest. The mechanism by which MBP increases solubility is not well understood. In addition, MBP can itself be used as an affinity tag for purification of recombinant proteins. The fusion protein binds to amylose columns while all other proteins flow through. The MBP-protein fusion can be purified by eluting the column with maltose. Once the fusion protein is obtained in purified form, the protein of interest (X) is often cleaved from MBP with a specific protease. Protein X can then be separated from MBP by affinity chromatography.The fusion of proteins with MBP usually enhances their solubility and facilitates their proper folding so that the fusion proteins are most often bifunctional. In addition, such fusions can facilitate the crystallisation of difficult proteins, e.g. membrane proteins.

Anti-MBP Tag Mouse Monoclonal Antibody (9Y5) was was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody has been tested with WB application. And Abbkine suggested starting dilutions are as follows: WB 1:5000.

Responsible to say, Anti-MBP Tag Mouse Monoclonal Antibody (9Y5) is a very good product. It works well. And this product is what I want.

PurKine™ MBP-Tag Dextrin Resin 6FF Products Released!

Dextrin Resins is a chromatography medium for purifying proteins fused to maltose binding protein (MBP-tagged protein). Recombinant proteins with MBP-tags often gives increased expression levels and higher solubility of the target protein. Proper folding of the attached protein has also been shown to be promoted by the MBP tag. Since MBP increase the solubility, the tag is extremely useful for recombinant proteins accumulated in an insoluble form (inclusion bodies).

PurKine™ MBP-Tag Dextrin Resin 6FF effectively purifies high levels of overexpressed MBP-tagged fusion proteins at a variety of scales. The Resin consists of 90μm beads of highly cross-linked 6% agarose, to which Dextrin has been coupled. Tests confirm that performance equals or exceeds popular Dextrin resins from other suppliers, and no decrease in performance occurs after at least five repeated uses. In addition, its high flow properties make it excellent for scaling-up.

The series of PurKine™ MBP-Tag Dextrin Resin products contain MBP-Tag Dextrin Resin 6FF, MBP-Tag Dextrin Packed Column 6FF, MBP-Tag Protein Purification Kit (Dextrin). Per milliliter of resin can capture more than 10 mg of MBP-tagged protein. MBP-Tag Dextrin Resin 6FF is a highly crosslinked resin which can tolerate at most 500cm/hour linear flow rates, thus it make  MBP-Tag Dextrin Resin 6FF more excellent for scaling-up.

Anti-Myc Tag Mouse Monoclonal Antibody (2D5) Review

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. The peptide sequence of the myc-tag is (in 1- and 3-letter codes, respectively): N-EQKLISEEDL-C, N-Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu-C, where N stands for Amino-terminus and C stands for Carboxy terminus. It can be fused to the C-terminus and the N-terminus of a protein. The tag is approximately 1202 Daltons in atomic mass and has 10 amino acids.

Anti-Myc Tag Mouse Monoclonal Antibody (2D5) was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody has been tested with WB, IP and IF. And Abbkine suggested starting dilutions are as follows: WB 1:5000, IP: 1:200, IF: 1:1000.

Abbkine assures the top-ranking production technology and service system, and provides the quality products with logical prices. I trust the brand very much. Anti-Myc Tag Mouse Monoclonal Antibody (2D5) is one of the products that I think is very good.

New PurKine™ GST-Tag Glutathione Resin(Packed Column) 4FF

Glutathione S-transferase (GST) is a 26 kDa protein derived from Schistosoma japonicum. GST enzymes from various sources, both native and recombinantly expressed as fusion to the N-terminus of target proteins. Reduced glutathione (GSH), when immobilized as a ligand to agarose or other chromatography supports, enables high-yield and high-quality purification of recombinant proteins expressed as fusions with glutathione S-transferase (GST) or other glutathione binding proteins expressed in E. coli, insect cells and mammalian cells.

PurKine™ GST-Tag Glutathione Resin 4FF effectively purifies high levels of overexpressed GST-tagged fusion proteins at a variety of scales. The Resin consists of 90μm beads of cross-linked 4% agarose, to which reduced glutathione has been coupled. Tests confirm that performance equals or exceeds popular GST-Tag Purification Resins from other suppliers, and no decrease in performance occurs after at least five repeated uses. PurKine™ GST-Tag Glutathione Resin Packed Column 4FF is a column that is pre-packed with Glutathione Resin. There is no need to pack resin by users.

PurKine™ GST-Tag Purification system is based on innovative high-capacity matrix for convenient single-step purifications of recombinant glutathione S-transferase (GST) fusion proteins and other glutathione binding proteins. Per milliliter of resin can capture more than 10 mg of GST-tagged protein and target  proteins can be eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality. The difference between Glutathione Resin and  Glutathione Resin 4FF is the latter is a highly crosslinked resin which can tolerate at most 450cm/hour  linear flow rates, thus it make Glutathione Resin 4FF more excellent for scaling-up.