2019年8月22日星期四

Selection Strategy of Apoptosis Products- Late apoptosis

Apoptosis
is an orderly and programmed death followed by cells under the influence of
physiological or pathological factors in order to maintain internal environment
stability. Apoptosis occurs in cells. First, the cell volume shrinks and the
connection disappears. Then, the density of cytoplasm increases, mitochondrial
membrane potential disappears, permeability changes, cytochrome C is released
to cytoplasm, nuclear substance is concentrated, nuclear membrane nucleolus is broken,
DNA is degraded into fragments, and finally apoptotic bodies are formed and
engulfed by macrophages.





Late apoptosis





In cell apoptosis, especially in the late stage of apoptosis, chromosomal
DNA will be broken, resulting in a large number of sticky 3'-OH ends. Under the
action of deoxynucleotide terminal transferase (TdT), derivatives formed by
deoxyuridine triphosphate nucleotide (dUTP) and fluorescein can be labeled to
the 3'- end of DNA, namely deoxynucleotide terminal transferase mediated nick
end labeling (TUNEL). Normal or proliferating cells have few DNA breaks and can
rarely be stained. Therefore, TUNEL is the most commonly used method to detect
DNA fragmentation in late apoptosis.





Product recommendation






Product name

Cat#

TUNEL Apoptosis Detection Kit (Green
Fluorescence)

KTA2010

TUNEL Apoptosis Detection Kit (Orange
Fluorescence)

KTA2011




Product character





Abbkine's TUNEL apoptosis detection kit provides a complete set of
reagent components and optimized experimental scheme required for the
experiment. It is suitable for a variety of instruments such as fluorescence
enzyme labeling instrument, fluorescence microscope, flow cytometer, etc. It
can be used to detect adherent cells, suspended cells, paraffin-embedded tissue
sections, frozen sections and other sample types.





Apoptosis cannot be stopped once it
starts, it is a highly regulated process.





Apoptosis can be
initiated by two initial pathways. The internal initial pathway is activated by
non-receptor stimulation, such as DNA damage, endoplasmic reticulum stress,
metabolic stress, mitochondrial outer membrane permeability changes, such as
cytochrome C release. The external pathway starts by binding death receptor to
ligand (such as FasL, tumor necrosis factor TNF). After that, the two
approaches converge in caspase cascade reaction, and cell death is induced by
activating caspase protease or protein degrading enzyme. Anti-apoptotic
ligands, such as cytokines and growth factors, promote cell survival,
proliferation and differentiation through different signaling molecules such as
AKT and p90RSK and anti-apoptotic proteins such as Bcl-2 and Bcl-x. The withdrawal
of these cytokines and growth factors leads to cell death.





Product recommendation






Product name

Cat#

Application

Bax Mouse Monoclonal Antibody (6F11)

ABM40273

IF, IHC-p, WB

Bcl-2 Monoclonal Antibody

ABM0010

IF, IHC-p, WB

Bad Polyclonal Antibody

ABP55880

ELISA, IF, IHC-p, WB

p53 Monoclonal Antibody

ABM0016

IF, IHC-p, WB

FAS Polyclonal Antibody

ABP51327

ELISA, IF, IHC-p, WB

Caspase-3 Polyclonal Antibody

ABP52935

ELISA, IF, IHC-p, WB

Caspase-8
Monoclonal Antibody

ABM0053

IF, IHC-p, WB

Caspase 9 Monoclonal Antibody

ABM0028

IF, IHC-p, IP, WB

NFkB p65 Monoclonal Antibody

ABM40111

IF, IHC-p, IP, WB




Product character





Abbkine has selected the antibody of apoptosis series. After multiple strict verifications, it is suitable for a variety of cell applications and meets the research needs of most customers. The excellent results are as follows:





IHC analysis of paraffin-embedded human uterine tissue was performed using Bad polyclonal antibody diluted at 1: 200.




IF analysis of rat lung tissue was performed using Caspase-3 polyclonal antibody diluted at 1: 200.




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