Caspase-1 Polyclonal Antibody Review

Caspase-1 (also known as ICE) is a prototypical member of the caspase-family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa proenzyme. The pro-enzyme is matured by proteolysis to yield large (20 kDa) and small (10 kDa) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. To date the regulatory mechanism of caspase-1 activation and the role of caspase-1 in apoptosis are poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive proenzyme form.

Caspase-1 Polyclonal Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been tested with WB and ELISA. And Abbkine suggested starting dilutions are as follows: WB: 1:500-2000, ELISA 1：10000-20000.

Caspase-1 is a mediator of the inflammatory response by cleaving the inactive pro-IL1 beta at Asp116/Ala 117 to generate the active 17k mature form of IL-1 beta. We find the quality suitable for our experiments. I am very satisfied with Abbkine's service.