Anti-MBP Tag Mouse Monoclonal Antibody (9Y5) Review

MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest. The mechanism by which MBP increases solubility is not well understood. In addition, MBP can itself be used as an affinity tag for purification of recombinant proteins. The fusion protein binds to amylose columns while all other proteins flow through. The MBP-protein fusion can be purified by eluting the column with maltose. Once the fusion protein is obtained in purified form, the protein of interest (X) is often cleaved from MBP with a specific protease. Protein X can then be separated from MBP by affinity chromatography.The fusion of proteins with MBP usually enhances their solubility and facilitates their proper folding so that the fusion proteins are most often bifunctional. In addition, such fusions can facilitate the crystallisation of difficult proteins, e.g. membrane proteins.

Anti-MBP Tag Mouse Monoclonal Antibody (9Y5) was was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody has been tested with WB application. And Abbkine suggested starting dilutions are as follows: WB 1:5000.

Responsible to say, Anti-MBP Tag Mouse Monoclonal Antibody (9Y5) is a very good product. It works well. And this product is what I want.