【Promotion】Abbkine Selected Primary Antibody-Click for Promotion details.
Bcl-2 gene (B-cell lymphoma-2) is a member of Bcl-2 family, which is the earliest discovered apoptosis protein. It is an anti-apoptosis protein containing multiple BH domains. It is highly expressed in some cancer cells and selectively plays an anti-tumor role. Bcl-2 family proteins play an important role in the regulation of apoptosis by forming dimers with Bax and dimerizing themselves. When Bcl-2 protein is inhibited, its dimer with Bax decreases, leading to cell apoptosis. When Bcl-2 protein is overexpressed, heterodimers formed by Bcl-2 protein and Bax increase and apoptosis is inhibited. The balance between Bcl-2 and Bax proteins at the cell death signal checkpoint determines the survival or apoptosis of cells.
Fig: Bcl-2 and Cell apoptosis
We recommend Abbkine selected Bcl-2 antibody for WB, IHC-P and IF experiments in human, rats, mice and chickens.
【Promotion】Abbkine Selected Primary Antibody-Click for Promotion details.
Bcl-2 gene (B-cell lymphoma-2) is a member of Bcl-2 family, which is the earliest discovered apoptosis protein. It is an anti-apoptosis protein containing multiple BH domains. It is highly expressed in some cancer cells and selectively plays an anti-tumor role. Bcl-2 family proteins play an important role in the regulation of apoptosis by forming dimers with Bax and dimerizing themselves. When Bcl-2 protein is inhibited, its dimer with Bax decreases, leading to cell apoptosis. When Bcl-2 protein is overexpressed, heterodimers formed by Bcl-2 protein and Bax increase and apoptosis is inhibited. The balance between Bcl-2 and Bax proteins at the cell death signal checkpoint determines the survival or apoptosis of cells.
Fig: Bcl-2 and Cell apoptosis
We recommend Abbkine selected Bcl-2 antibody for WB, IHC-P and IF experiments in human, rats, mice and chickens.
【Promotion】Abbkine Selected Primary Antibody-Click for Promotion details.
Bcl-2 gene (B-cell lymphoma-2) is a member of Bcl-2 family, which is the earliest discovered apoptosis protein. It is an anti-apoptosis protein containing multiple BH domains. It is highly expressed in some cancer cells and selectively plays an anti-tumor role. Bcl-2 family proteins play an important role in the regulation of apoptosis by forming dimers with Bax and dimerizing themselves. When Bcl-2 protein is inhibited, its dimer with Bax decreases, leading to cell apoptosis. When Bcl-2 protein is overexpressed, heterodimers formed by Bcl-2 protein and Bax increase and apoptosis is inhibited. The balance between Bcl-2 and Bax proteins at the cell death signal checkpoint determines the survival or apoptosis of cells.
Fig: Bcl-2 and Cell apoptosis
We recommend Abbkine selected Bcl-2 antibody for WB, IHC-P and IF experiments in human, rats, mice and chickens.
Common methods of cell proliferation mainly include MTT method, WST-1 method and CCK-8 method, which are mainly based on the activity of cells, thus reflecting the overall proliferation effect, but unable to detect individual proliferating cells. Recognized as the most accurate method to detect cell proliferation is to directly detect DNA synthesis in cells. Previously, BrdU method was the most commonly used method. However, the method requires DNA denaturation (such as acid denaturation, thermal denaturation or DNase digestion) to expose BrdU, thus binding to BrdU antibody. The whole experiment has many influencing factors and poor stability.
Abbkine innovates and develops EdU method based on EdU incorporation and subsequent click reaction to solve this application difficulty:
Product name
Cat#
Fluorescent characteristic
Cell Proliferation EdU Image Kit (Green Fluorescence)
Principle：EdU (5-ethynyl-2´-deoxyuridine) is a nucleoside analog of thymidine and is incorporated into DNA during active DNA synthesis. Comparing to BrdU assays, the EdU-Click Assays are not antibody based and therefore do not require DNA denaturation (typically using HCl or heat or digestion with DNase) for detection of the incorporated nucleoside. Detection is based on a click reaction, a copper-catalyzed covalent reaction between an azide and an alkyne, is complete within 30 minutes.
Kit components
Advantage
• EdU (10mM) • AbFluor 488 azide or AbFluor 545 azide • 10×Reaction buffer • Copper • Reducing Agent
• Not antibody based • No DNA denaturation. Can maintain cell morphology and DNA integrity • Patented AbFluor 488 and 545 azide have good light stability and quenching resistance • Optimization for fluorescence microscope
Promotion product list: Classical loading control and tag antibodies and the conjugated versions of these antibodies, including HRP, Biotin, FITC, Cy3, Cy5 and AbFlour TM dyes.
Details of activities: Buy 2 get 1 for free (if a single order is full of 3 antibodies, the antibody with the lowest price will be exempted).
Why choose Abbkine loading control and tag antibodies?
Abbkine offers a wide range of loading control and tag antibodies, including the hot targets and unique species, covering the molecular weights from 15 kDa to 116 kDa.
Abbkine conjugated loading control and tag antibodies have diverse conjugates, including enzyme and featured fluorescent dyes, with the application of WB, IHC, IF and IP experiments.
Abbkine loading control and tag antibodies are verified through rigorous procedures, popular with customers for their good performance and high sensitivity.
Different sizes for clients to choose, small size and bulk size are available, both with high cost-effectiveness.
Part of promotion product list:
Classical loading control and tag antibodies (Citation of over 100 SCI Documents)
IP experiment partner-Agarose/Magnetic beads conjugated tag antibodies
Compared with the traditional immunoprecipitation (IP) method using Protein A/G, Abbkine's agarose/magnetic bead coupling labeled antibody eliminates the step of Protein A/G binding with antigen-antibody complex. After the conjugated antibody is directly combined with the target protein, the target protein is separated from the cell lysate by centrifugation or using a magnetic rack. It not only makes the experiment simple and fast, but also can avoid non-specific binding and reduce the background.
For the full product promotion list, please contact Abbkine's local distributor or contact service@abbkine.com directly. Abbkine has the final right to interpret this activity.
Promotion product list: Classical loading control and tag antibodies and the conjugated versions of these antibodies, including HRP, Biotin, FITC, Cy3, Cy5 and AbFlour TM dyes.
Details of activities: Buy 2 get 1 for free (if a single order is full of 3 antibodies, the antibody with the lowest price will be exempted).
Why choose Abbkine loading control and tag antibodies?
Abbkine offers a wide range of loading control and tag antibodies, including the hot targets and unique species, covering the molecular weights from 15 kDa to 116 kDa.
Abbkine conjugated loading control and tag antibodies have diverse conjugates, including enzyme and featured fluorescent dyes, with the application of WB, IHC, IF and IP experiments.
Abbkine loading control and tag antibodies are verified through rigorous procedures, popular with customers for their good performance and high sensitivity.
Different sizes for clients to choose, small size and bulk size are available, both with high cost-effectiveness.
Part of promotion product list:
Classical loading control and tag antibodies (Citation of over 100 SCI Documents)
IP experiment partner-Agarose/Magnetic beads conjugated tag antibodies
Compared with the traditional immunoprecipitation (IP) method using Protein A/G, Abbkine's agarose/magnetic bead coupling labeled antibody eliminates the step of Protein A/G binding with antigen-antibody complex. After the conjugated antibody is directly combined with the target protein, the target protein is separated from the cell lysate by centrifugation or using a magnetic rack. It not only makes the experiment simple and fast, but also can avoid non-specific binding and reduce the background.
For the full product promotion list, please contact Abbkine's local distributor or contact service@abbkine.com directly. Abbkine has the final right to interpret this activity.
Apoptosis is an orderly and programmed death followed by cells under the influence of physiological or pathological factors in order to maintain internal environment stability. Apoptosis occurs in cells. First, the cell volume shrinks and the connection disappears. Then, the density of cytoplasm increases, mitochondrial membrane potential disappears, permeability changes, cytochrome C is released to cytoplasm, nuclear substance is concentrated, nuclear membrane nucleolus is broken, DNA is degraded into fragments, and finally apoptotic bodies are formed and engulfed by macrophages.
Late apoptosis
In cell apoptosis, especially in the late stage of apoptosis, chromosomal DNA will be broken, resulting in a large number of sticky 3'-OH ends. Under the action of deoxynucleotide terminal transferase (TdT), derivatives formed by deoxyuridine triphosphate nucleotide (dUTP) and fluorescein can be labeled to the 3'- end of DNA, namely deoxynucleotide terminal transferase mediated nick end labeling (TUNEL). Normal or proliferating cells have few DNA breaks and can rarely be stained. Therefore, TUNEL is the most commonly used method to detect DNA fragmentation in late apoptosis.
Abbkine's TUNEL apoptosis detection kit provides a complete set of reagent components and optimized experimental scheme required for the experiment. It is suitable for a variety of instruments such as fluorescence enzyme labeling instrument, fluorescence microscope, flow cytometer, etc. It can be used to detect adherent cells, suspended cells, paraffin-embedded tissue sections, frozen sections and other sample types.
Apoptosis cannot be stopped once it starts, it is a highly regulated process.
Apoptosis can be initiated by two initial pathways. The internal initial pathway is activated by non-receptor stimulation, such as DNA damage, endoplasmic reticulum stress, metabolic stress, mitochondrial outer membrane permeability changes, such as cytochrome C release. The external pathway starts by binding death receptor to ligand (such as FasL, tumor necrosis factor TNF). After that, the two approaches converge in caspase cascade reaction, and cell death is induced by activating caspase protease or protein degrading enzyme. Anti-apoptotic ligands, such as cytokines and growth factors, promote cell survival, proliferation and differentiation through different signaling molecules such as AKT and p90RSK and anti-apoptotic proteins such as Bcl-2 and Bcl-x. The withdrawal of these cytokines and growth factors leads to cell death.
Abbkine has selected the antibody of apoptosis series. After multiple strict verifications, it is suitable for a variety of cell applications and meets the research needs of most customers. The excellent results are as follows:
IHC analysis of paraffin-embedded human uterine tissue was performed using Bad polyclonal antibody diluted at 1: 200.
IF analysis of rat lung tissue was performed using Caspase-3 polyclonal antibody diluted at 1: 200.
