CheKine™ Xanthine Oxidase Assay Kit, a great tool for study of hyperuricemia

Xanthine oxidase is a form of xanthine oxidoreductase, a type of enzyme that generates reactive oxygen species. These enzymes catalyze the oxidation of hypoxanthine to xanthine and can further catalyze the oxidation of xanthine to uric acid. These enzymes play an important role in the catabolism of purines in some species, including humans.

Xanthine oxidase (XO, EC 1.17.3.2 ) is present in appreciable amounts in the liver and jejunum in healthy individuals. Most of the protein in the liver exists in a form with xanthine dehydrogenase activity, but it can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. In various liver disorders, XO is released into circulation. Therefore, determination of serum XO level serves as a sensitive indicator of acute liver damage such as jaundice.

Abbkine CheKine™ Xanthine Oxidase Assay Kit provides a simple and easy colorimetric assay for the quantitative determination of the XO present in a variety of samples. In the assay, XO oxidizes xanthine to superoxide (O2 - ). Superoxide (O2 - ) reacts with a tetrazolium salt WST-8 dye to form a water-soluble colored formazan product, which can be easily quantified colorimetrically at OD 450 nm. Therefore, the Xanthine Oxidase present in the sample is proportional to the signal obtained.

Product Name	CAT #	Detection Range
CheKine™ Xanthine Oxidase Assay Kit	KTB1070	15.6 -500 mU/mL

【Kit components】

•
Assay Buffer

•
Sample Diluent

•
WST-8

•
Enhancer

•
Xanthine Oxidase Standard (10 U/mL)

•
Xanthine

【Procedure】

1. Prepare
   the Working Reagent.
2. Add
   standard and sample per well. Then add Working Reagent per well quickly. Tap
   plate to mix briefly and thoroughly. Immediately read
   optical density at 450nm (OD0).
3. Incubate for 30 min at room
   temperature (25°C) in the dark. Read optical density at 450nm again (OD30).

【Standard Curve】

About Abbkine Scientific Co., Ltd.

Abbkine serves global scientists in the field of
proteomics and cytology and is committed to the innovation and development of
various scientific reagents related to proteomics and cytology, expecting to
accelerate the pace of life science research and drug discovery. Proteomics
products cover the preparation of samples (protein extraction, purification,
coupling), protein quantification, antibodies and kits for protein detection.
Cytology products involve cytokines (cell culture), cell status detection, cell
staining, organelle extraction, cell metabolism and cytopathology reagents
(kits). Abbkine relies on the product portfolio and unique marketing support as
the main market strategy and product innovation mode, with ultimate aim to
facilitate your research career.

New biosensor weapons, CheKine™ Glucose Oxidase Activity (GOD)Assay Kit

Glucose oxidase is a an oxido-reductase
that catalyses the oxidation of glucose to hydrogen peroxide and
D-glucono-δ-lactone. This enzyme is produced by certain species of fungi and
insects and displays antibacterial activity when oxygen and glucose are
present. Glucose oxidase is widely used to measure glucose in body fluids, as
well as residual glucose and oxygen from beverages, foods and other
agricultural products. In addition, glucose oxidase is commonly used as
biosensors to detect glucose.

Test the activity of glucose oxidase
(GOD), recommend the Abbkine CheKine™ glucose oxidase (GOD) activity kit for
you, details are as follows:

Product Name	CAT #	Detection Range
CheKine™ Glucose Oxidase Activity (GOD)Assay Kit	KTB1310	0.05 - 2 U/L

【Principle】

In this assay, GOD activity
is determined by a coupled enzyme assay, in which GOD oxidizes D-glucose
resulting in the production of hydrogen peroxide (H2O2) that reacts with
chromogen in an acid condition, produce a product that can be measured at OD
580 nm. Therefore, the glucose oxidase activity present in the sample is
proportional to the signal obtained.

【Sample】

Serum, Plasma, Cells, Tissues, Urine, Food,
Medium, other body fluids.

【Kit components】

• Assay Buffer (20x)
• Glucose (0.2 M)
• H2O2 Standard (0.88 M)
• Chromogen
• Glucose Oxidase (control)

【Simple procedure】

1. Prepare standards and sample, add Glucose
   to each tube, mix and incubate for 20 min at 37°C.
2. Transfer the mixture into a clear bottom
   96-well plate, add Chromogen per well, mix and read optical density at 580nm(OD0).
   Incubate for 10 min at 37°C in the dark. Read optical density at 580nm again(OD10).

【Standard Curve】

About Abbkine Scientific Co., Ltd.

Abbkine serves global scientists in the
field of proteomics and cytology and is committed to the innovation and
development of various scientific reagents related to proteomics and cytology,
expecting to accelerate the pace of life science research and drug discovery.
Proteomics products cover the preparation of samples (protein extraction,
purification, coupling), protein quantification, antibodies and kits for
protein detection. Cytology products involve cytokines (cell culture), cell
status detection, cell staining, organelle extraction, cell metabolism and
cytopathology reagents (kits). Abbkine relies on the product portfolio and
unique marketing support as the main market strategy and product innovation mode,
with ultimate aim to facilitate your research career.

CheKine™ Lactate Assay Kit, fast quantitative determination of L (+)-Lactate in various samples

Lactate
(CH3CH(OH)COO-) is a metabolic compound formed in animals by the action of the enzyme
lactate dehydrogenase. Lactate is produced in proliferating cells during
anaerobic conditions such as exercise. Abnormally high concentrations of
lactate have been related to pathological conditions such as cancer, diabetes,
and lactate acidosis.

L(+)-Lactate is the major lactate stereoisomer formed in human intermediary metabolism and is present in blood at levels of around 1-2 mmol/L. D(-)-Lactate can also be found in blood but only at about 1-5% of the concentration of L(+)-Lactate.

Lactic
acid is the end product of glycolysis in the body, which can be produced when
tissue hypoxia or pyruvic acid is not oxidized in time. Usually, the lactic
acid obtained from metabolism does not affect the acid-base balance in the
patient's body. However, when the violent exercise reaches a high metabolic
state or shock, the anaerobic metabolism in the tissue increases significantly,
accelerating the production of lactic acid. The body's ability to remove lactic
acid is gradually reduced, eventually leading to hyperlactacidemia, even lactic
acidosis. At this time, the blood lactate level test results showed a
significant increase. So the  lactate
level can be used as an important indicator to reflect the balance of oxygen supply
and demand in critically ill patients.

Want a quick, quantitative determination of lactic acid? Abbkine CheKine™ Lactate assay kit will give you a good experience!

Abbkine CheKine™ Lactate Assay Kit provides a convenient means for detecting L-(+)-Lactate in biological samples such as in serum or plasma, cells, culture and fermentation media. In this kit lactate is oxidized by lactate dehydrogenase to generate a product which interacts with a tetrazolium salt WST-8 dye to form a colorimetric (450 nm) product, proportional to the lactate present.

Product Name	CAT #	Detection Range
CheKine™ Lactate Assay Kit	KTB1100	Detection Range

【Kit components】

• Lactate
Assay Buffer

• Lactate
Dehydrogenase

• Lactate
Dehydrogenase Cofactor

•
WST-8

• Enhancer

•
L(+)-Lactate Standard (100 mM)

【Procedure】

1. Prepare
   the Working Reagent.
2. Add
   diluted standard and sample per well, then add Working Reagent. Tap plate
   to mix. Immediately read optical density at 450nm (OD0).
3. Incubate
   for 30 min at 37°C in the dark. Read optical density at 450nm again (OD30).

【Standard Curve】

About Abbkine Scientific Co., Ltd.

Abbkine serves global scientists in the field of proteomics and cytology and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, expecting to accelerate the pace of life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, with ultimate aim to facilitate your research career.

CheKine™ Lactate Assay Kit, fast quantitative determination of L (+)-Lactate in various samples

Lactate
(CH3CH(OH)COO-) is a metabolic compound formed in animals by the action of the enzyme
lactate dehydrogenase. Lactate is produced in proliferating cells during
anaerobic conditions such as exercise. Abnormally high concentrations of
lactate have been related to pathological conditions such as cancer, diabetes,
and lactate acidosis.

L(+)-Lactate is the major lactate stereoisomer formed in human intermediary metabolism and is present in blood at levels of around 1-2 mmol/L. D(-)-Lactate can also be found in blood but only at about 1-5% of the concentration of L(+)-Lactate.

Lactic
acid is the end product of glycolysis in the body, which can be produced when
tissue hypoxia or pyruvic acid is not oxidized in time. Usually, the lactic
acid obtained from metabolism does not affect the acid-base balance in the
patient's body. However, when the violent exercise reaches a high metabolic
state or shock, the anaerobic metabolism in the tissue increases significantly,
accelerating the production of lactic acid. The body's ability to remove lactic
acid is gradually reduced, eventually leading to hyperlactacidemia, even lactic
acidosis. At this time, the blood lactate level test results showed a
significant increase. So the  lactate
level can be used as an important indicator to reflect the balance of oxygen supply
and demand in critically ill patients.

Want a quick, quantitative determination of lactic acid? Abbkine CheKine™ Lactate assay kit will give you a good experience!

Abbkine CheKine™ Lactate Assay Kit provides a convenient means for detecting L-(+)-Lactate in biological samples such as in serum or plasma, cells, culture and fermentation media. In this kit lactate is oxidized by lactate dehydrogenase to generate a product which interacts with a tetrazolium salt WST-8 dye to form a colorimetric (450 nm) product, proportional to the lactate present.

Product Name	CAT #	Detection Range
CheKine™ Lactate Assay Kit	KTB1100	Detection Range

【Kit components】

• Lactate
Assay Buffer

• Lactate
Dehydrogenase

• Lactate
Dehydrogenase Cofactor

•
WST-8

• Enhancer

•
L(+)-Lactate Standard (100 mM)

【Procedure】

1. Prepare
   the Working Reagent.
2. Add
   diluted standard and sample per well, then add Working Reagent. Tap plate
   to mix. Immediately read optical density at 450nm (OD0).
3. Incubate
   for 30 min at 37°C in the dark. Read optical density at 450nm again (OD30).

【Standard Curve】

About Abbkine Scientific Co., Ltd.

Abbkine serves global scientists in the field of proteomics and cytology and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, expecting to accelerate the pace of life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, with ultimate aim to facilitate your research career.

Want High accuracy and specificity? Yes, it’s Abbkine CheKine™ Catalase (CAT) Activity Assay Kit with great value

【Background】

Catalase is a common enzyme found in nearly all living organisms exposed to oxygen (such as bacteria, plants, and animals). It catalyzes the decomposition of hydrogen peroxide to water and oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS). Likewise, catalase has one of the highest turnover numbers of all enzymes; one catalase molecule can convert millions of hydrogen peroxide molecules to water and oxygen each second.

Catalase is a common enzyme found in nearly all living organisms exposed to oxygen (such as bacteria, plants, and animals). It catalyzes the decomposition of hydrogen peroxide to water and oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS). Likewise, catalase has one of the highest turnover numbers of all enzymes; one catalase molecule can convert millions of hydrogen peroxide molecules to water and oxygen each second.

Catalase is a tetramer of four polypeptide
chains, each over 500 amino acids long. It contains four iron-containing heme
groups that allow the enzyme to react with the hydrogen peroxide. The optimum
pH for human catalase is approximately and has a fairly broad maximum, the rate
of reaction does not change appreciably between pH 6.8 and 7.5. The pH optimum
for other catalases varies between 4 and 11 depending on the species. The
optimum temperature also varies by species.

【Abbkine Catalase Activity Assay Kit】

Abbkine Catalase
Activity Assay Kit utilizes the peroxidatic
function of catalase for measuring

catalase activity, based on the reaction of catalase with
methanol, with the presence of an optimal concentration of H₂O₂.
The formaldehyde produced can be measured colorimetrically at OD 540 nm.
Therefore, the catalase activity present in the sample is proportional to the signal
obtained.

Most commercial
Catalase Activity Assay Kits apply this principle that residual
hydrogen peroxide is oxidized by peroxidase to form the colored substrate, and the
catalase activity was detected indirectly. However, other peroxidases in the
sample may interfere with the experimental results and this method may cause
standard curve errors due to unstable hydrogen peroxide.

Abbkine
kit has unique advantages, which can detect the enzyme activity directly, with
better more veracity and higher specificity. Since methanol is a unique
substrate of catalase, other peroxidases cannot use methanol as a substrate, so
interference signals are avoided.

Product name	Cat#	Detection Range
CheKine™ Catalase (CAT) Activity Assay Kit	KTB1040	2-75 µM

【Kit components】

• Assay Buffer (10x)

• Sample Diluent (10x)

• Formaldehyde standard (4.25
M)

• Catalase (positive control)

• Potassium Hydroxide

• Hydrogen Peroxide

• Chromogen

• Potassium Periodate

【Features & Benefits】

• Determination of catalase
  activity in serum, plasma, tissue/cell lysates and other biological fluids.
• A
  broad range linearity of 2-75 µM, measure catalase activity down to
  2 U/ml
• Determining
  catalase activity directly by utilizing the peroxidase function of catalase,
  which cannot be interfered by other peroxidases. 
• Simple
  operation, only four steps to complete the measurement

【Results Analysis】

Formaldehyde (µM)

1. Calculate the formaldehyde
   concentration of the samples using the equation obtained from standard
   
   

2）Calculate the CAT activity of the sample using the following
equation. One unit is defined as the amount of enzyme that will cause the
formation of 1.0 nmol of formaldehyde per minute at 25°C

Note: µM means nmol/ml

Please refer to Product datasheet: https://www.abbkine.com/file/booklet/KTB1040-B.pdf

About Abbkine Scientific Co., Ltd.

Abbkine serves global scientists in the
field of proteomics and cytology and is committed to the innovation and
development of various scientific reagents related to proteomics and cytology,
expecting to accelerate the pace of life science research and drug discovery.
Proteomics products cover the preparation of samples (protein extraction,
purification, coupling), protein quantification, antibodies and kits for
protein detection. Cytology products involve cytokines (cell culture), cell
status detection, cell staining, organelle extraction, cell metabolism and
cytopathology reagents (kits). Abbkine relies on the product portfolio and
unique marketing support as the main market strategy and product innovation
mode, with ultimate aim to facilitate your research career.

Observing three-dimensional dynamics of the nucleus in live cells, Abbkine will give you great experiences !

Live-cell imaging technique allows
real-time examination of almost every aspect of cellular function under normal
and experimental conditions, so it is especially popular with cytology
researchers. Abbkine TraKine™ Pro is a series of cell-permeable organic fluorescent
probes which can selectively recognize and label organelles. Currently, we
launched the staining kits involve tubulin, lysosome, mitochondrion and nuclei.
This paper introduces the TraKine™ Pro Live-cell Nuclei Staining kit (Deep Red
Fluorescence with Super Resolution).

Function of nucleus

The nucleus is the largest and most important cell structure in eukaryotic cells, mainly composed of nuclear envelope, chromatin, nuclear skeleton, nucleolus and nucleosome. Nuclear membrane is a two-layer membrane that completely encloses the nucleus and separates the material within the membrane from the cytoplasm and lamina.

The nucleus contains most of the cell’s
genome, organized as multiple long linear DNA molecules in a complex with a
large variety of proteins, such as histones, to form chromosomes. It is the
regulatory center of cell heredity and metabolism, and plays an important role
in cell metabolism, growth and differentiation.

Commercially available nucleus fluorescent probes

The
nucleus is the place where the genetic material is stored and reproduced, and
it is also the control center of cellular heredity and cell metabolism. Studying
its function, mechanism, dynamics and interaction is important direction for
cytology researchers.

At
present, the existing nuclear dyes (such as PI, DAPI, ethd-1, etc.) are mostly
used for dying cell staining, apoptosis research, nuclear counterstaining, and
so on. Hoechst dye is a compound synthesized by Hoechst AG originally, which is
soluble in water and organic solvents. It can cross cell membranes and bind to
living or fixed cells, the fluorescence intensity enhanced along with the increase
of the pH value of the solution.

Product name	Cat#	Ex/Em（nm）	Application
DAPI	BMD00063	358/461 (with DNA)	Dead cell staining; counterstaining; Apoptosis study
Hoechst 33258	BMD00061	352/461 (with DNA)	Live or fixed cells; Apoptosis study
Hoechst 33342	BMD00062	350/461 (with DNA)	Live cells or tissues; fixed cells or tissue; Apoptosis study
Ethidium Homodimer-1 (EthD-1)	BMD00060	528/617 (with DNA)	Dead cell staining; High affinity fluorescent nucleic acid dyes, binding DNA or RNA

However,
there is no dye that can be used to observe the dynamics of living cell nucleus
very well currently. Abbkine researchers developed a unique TraKine™ Pro
fluorescent probe that can track the 3d dynamics of living cell nucleus for a
long time with ultra-high resolution.

The TraKine™ Pro nuclear fluorescence probe has been
specially optimized, which contains a fluorescent dye and a small molecular
group that specifically identifies the nucleus, to locate the nucleus and track
its dynamics and changes. It is the only highly specific live cell nuclear
staining product on the market.

Product name	Cat#	Ex/Em（nm）	Live or fixed cells	Microscope compatibility
TraKine™ Pro Live-cell Nuclei Staining kit (Deep Red Fluorescence with Super Resolution)	KTC4510	650/665	Live& fixed cells	Fluorescence microscope，Confocal, SIM, PALM, STORM, TIRF

【Features & Benefits】

1 Optimized staining protocol for labeling subcellular structures in mammalian living and fixed cells.
2 Super resolution, especially suitable for Confocal and long-term super-resolution imaging (such as SIM, STED, TIRF, STORM and PALM).
3 Fluorescence can last for several hours in cells, stable and persistent, especially ideal for monitoring dynamics
4 High specificity, low background, excellent photostability and good cell permeability.
5 Safe, low levels of cytotoxicity to cells

【Perfect
results are the best illustration】

KTC4510 SIM imaging: U2OS cells	KTC4510 Confocal imaging: U2OS cells

About Abbkine Scientific Co., Ltd.

Abbkine serves global scientists in the
field of proteomics and cytology and is committed to the innovation and
development of various scientific reagents related to proteomics and cytology,
expecting to accelerate the pace of life science research and drug discovery.
Proteomics products cover the preparation of samples (protein extraction,
purification, coupling), protein quantification, antibodies and kits for
protein detection. Cytology products involve cytokines (cell culture), cell
status detection, cell staining, organelle extraction, cell metabolism and
cytopathology reagents (kits). Abbkine relies on the product portfolio and
unique marketing support as the main market strategy and product innovation
mode, with ultimate aim to facilitate your research career.

Caspase(1,2,3,4,6,8,9) Assay Kit- Simple and convenient, to boost your apoptosis study

【Tips】If you are studying whether a certain drug can trigger apoptosis which relys on the caspase related pathway, and want to detect the activity of caspase, this article is suitable for you! This article is especially fit for researchers who are working hard to benefit the mankind.

【Background】

Caspases(cysteine-aspartic proteases) are a
family of protease enzymes playing essential roles in programmed cell death
(including apoptosis, pyroptosis and necroptosis) and inflammation. The cascade
reaction caused by caspases is a central part of the apoptotic process, and its
activation mainly includes the mitochondria-dependent pathway and the death
receptor-mediated signal transduction pathway. The downstream caspases are then
activated, leading to apoptosis by cleavage of specific substrates. Inhibition
of apoptotic function will lead to tumorigenesis and abnormal immune function.
However, hyperfunction of apoptotic function is closely related to the
pathogenesis of tissue damage and organ failure.

【Caspase Structure】

【Assay Principle】

Abbkine Caspase Assay Kits are based on different
caspases can catalyze specific substrates to generate pNA(p-nitroaniline), resulting
in the release of p-nitroaniline (pNA), which can be quantified using a
spectrophotometer or a microtiter plate reader at at 405 nm.

Product name	Cat#
Caspase-1 Assay Kit (Colorimetric)	KTA3020
Caspase-2 Assay Kit (Colorimetric)	KTA3021
Caspase-3 Assay Kit (Colorimetric)	KTA3022
Caspase-4 Assay Kit (Colorimetric)	KTA3023
Caspase-6 Assay Kit (Colorimetric)	KTA3024
Caspase-8 Assay Kit (Colorimetric)	KTA3025
Caspase-9 Assay Kit (Colorimetric)	KTA3026

【Assay Procedure】

1. Add samples, background control
   and standard
2. Add Reaction Buffer and
   substrate, incubate
3. Read samples at 405 nm

Simple
three steps, a big step in scientific research!

About Abbkine Scientific Co., Ltd.

Abbkine serves global scientists in the
field of proteomics and cytology and is committed to the innovation and
development of various scientific reagents related to proteomics and cytology,
expecting to accelerate the pace of life science research and drug discovery.
Proteomics products cover the preparation of samples (protein extraction,
purification, coupling), protein quantification, antibodies and kits for
protein detection. Cytology products involve cytokines (cell culture), cell
status detection, cell staining, organelle extraction, cell metabolism and
cytopathology reagents (kits). Abbkine relies on the product portfolio and
unique marketing support as the main market strategy and product innovation
mode, with ultimate aim to facilitate your research career.