Xanthine oxidase is a form of xanthine oxidoreductase, a type of enzyme that generates reactive oxygen species. These enzymes catalyze the oxidation of hypoxanthine to xanthine and can further catalyze the oxidation of xanthine to uric acid. These enzymes play an important role in the catabolism of purines in some species, including humans.
Xanthine oxidase (XO, EC 1.17.3.2 ) is present in appreciable amounts in the liver and jejunum in healthy individuals. Most of the protein in the liver exists in a form with xanthine dehydrogenase activity, but it can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. In various liver disorders, XO is released into circulation. Therefore, determination of serum XO level serves as a sensitive indicator of acute liver damage such as jaundice.
Abbkine CheKine™ Xanthine Oxidase Assay Kit provides a simple and easy colorimetric assay for the quantitative determination of the XO present in a variety of samples. In the assay, XO oxidizes xanthine to superoxide (O2 - ). Superoxide (O2 - ) reacts with a tetrazolium salt WST-8 dye to form a water-soluble colored formazan product, which can be easily quantified colorimetrically at OD 450 nm. Therefore, the Xanthine Oxidase present in the sample is proportional to the signal obtained.
Product Name | CAT # | Detection Range |
CheKine™ Xanthine Oxidase Assay Kit | KTB1070 | 15.6 -500 mU/mL |
【Kit components】
•
Assay Buffer
•
Sample Diluent
•
WST-8
•
Enhancer
•
Xanthine Oxidase Standard (10 U/mL)
•
Xanthine
【Procedure】
- Prepare
the Working Reagent. - Add
standard and sample per well. Then add Working Reagent per well quickly. Tap
plate to mix briefly and thoroughly. Immediately read
optical density at 450nm (OD0). - Incubate for 30 min at room
temperature (25°C) in the dark. Read optical density at 450nm again (OD30).
【Standard Curve】
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