New biosensor weapons, CheKine™ Glucose Oxidase Activity (GOD)Assay Kit

Glucose oxidase is a an oxido-reductase
that catalyses the oxidation of glucose to hydrogen peroxide and
D-glucono-δ-lactone. This enzyme is produced by certain species of fungi and
insects and displays antibacterial activity when oxygen and glucose are
present. Glucose oxidase is widely used to measure glucose in body fluids, as
well as residual glucose and oxygen from beverages, foods and other
agricultural products. In addition, glucose oxidase is commonly used as
biosensors to detect glucose.

Test the activity of glucose oxidase
(GOD), recommend the Abbkine CheKine™ glucose oxidase (GOD) activity kit for
you, details are as follows:

Product Name	CAT #	Detection Range
CheKine™ Glucose Oxidase Activity (GOD)Assay Kit	KTB1310	0.05 - 2 U/L

【Principle】

In this assay, GOD activity
is determined by a coupled enzyme assay, in which GOD oxidizes D-glucose
resulting in the production of hydrogen peroxide (H2O2) that reacts with
chromogen in an acid condition, produce a product that can be measured at OD
580 nm. Therefore, the glucose oxidase activity present in the sample is
proportional to the signal obtained.

【Sample】

Serum, Plasma, Cells, Tissues, Urine, Food,
Medium, other body fluids.

【Kit components】

• Assay Buffer (20x)
• Glucose (0.2 M)
• H2O2 Standard (0.88 M)
• Chromogen
• Glucose Oxidase (control)

【Simple procedure】

1. Prepare standards and sample, add Glucose
   to each tube, mix and incubate for 20 min at 37°C.
2. Transfer the mixture into a clear bottom
   96-well plate, add Chromogen per well, mix and read optical density at 580nm(OD0).
   Incubate for 10 min at 37°C in the dark. Read optical density at 580nm again(OD10).

【Standard Curve】

About Abbkine Scientific Co., Ltd.

Abbkine serves global scientists in the
field of proteomics and cytology and is committed to the innovation and
development of various scientific reagents related to proteomics and cytology,
expecting to accelerate the pace of life science research and drug discovery.
Proteomics products cover the preparation of samples (protein extraction,
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with ultimate aim to facilitate your research career.