ATG7 Mouse Monoclonal Antibody (3D6) Review

ATG7 gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance.

ATG7 Mouse Monoclonal Antibody (3D6) was affinity-purified from mouse ascites by affinity-chromatography using the recombinant protein of ATG7 immunogen. The effects in IF and IHC-P experiments had been well validated. This antibody can be used to detect endogenous ATG7 protein in the sample of Human, Mouse and Rat. According to your own experimental conditions, optimal working dilutions should be explored. The suggested starting dilution for IHC is 1:50-200.

Choosing a good antibody always bring you nice experience. I purchased several products form Abbkine, and have to say this is a trustworthy brand. Abbkine primary antibodies choose synthetic peptides instead of full-length proteins as immunogen, which testifies good results in the research experiments, especially in IF and IHC immunological tests. I conducted immunohistochemical analysis in rat lung tissue by using this antibody, gaining perfect results. The antibody was diluted at 1:200. I really appreciate this experience, I will continue to support Abbkine products.