2017年8月31日星期四

Abbkine Scientific launches the Cytokeratin 6 Monoclonal Antibody

Abbkine Scientific launches the Cytokeratin 6 Monoclonal AntibodyCytokeratin 6 Monoclonal Antibody or Cytokeratin 6 antibody as it also called is another unique scientific product manufactured by Chinese-based scientific research company. The research product is designed to allow for the easy detection of endogenous Cytokeratin 6 proteins.


The Keratin 6B belongs to the keratin gene family, with type II of cytokeratin consisting of basic or neutral proteins arranged in pairs of heterotypic keratin chains. The product has been identified as an effective research product, with the makers expressly stating that it is made exclusively for research purpose and not intended for human or diagnostic use.


The Keratin antibody is synthetic peptide immunogen with human reactivity. It can be applied in IF, IHC-p, WB and it is advised that the optimal working dilutions are to be determined through experiments by the investigator. The researcher at Abbkine have however suggested starting dilutions of WB: 1:1000.


The monoclonal antibody, CK 6A antibody, is hosted in the mouse, with a Mouse IgG1 isotype and went through state-of-the-art purification process. The product was affinity-purified from mouse ascites by affinity-chromatography with the use of specific immunogen.


The product is available in a liquid solution with a 1 mg/ml concentration and can be stored for up to twelve months from the date of shipment if stored at a stable temperature of -20 °C.


About Abbkine Scientific


Abbkine Scientific has grown to become a household name in the field of life science research, providing top-notch research tools and products to investigators and scientific researchers across the globe.


The company was established in 2012 in United States but moved its headquarters to China in a bid to meet growing demand from the region.

2017年8月30日星期三

Unexpected sensitive and accurate EliKine ELISA Kits

Abbkine newly launched EliKine™ series of ELISA kits exert high sensibility and specifcity, with a comprehensive selection of ELISA kits available for the quantifcation of cytokines, hormones and

other proteins, to meet your multiple experiment demands.


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EliKine™ Human CCL2 ELISA Kit Review

EliKine™ Human CCL2 ELISA Kit ReviewThe chemokine (C-C motif) ligand 2 (CCL2) is also referred to as monocyte chemoattractant protein 1 (MCP1) and small inducible cytokine A2. CCL2 is a small cytokine that belongs to the CC chemokine family. CCL2 recruits monocytes, memory T cells, and dendritic cells to the sites of inflammation produced by either tissue injury or infection. In the human genome, CCL2 and many other CC chemokines are located on chromosome 17 (17q11.2-q21.1). The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.


Human CCL2 ELISA Kit employs a two-site sandwich ELISA to quantitate CCL2 in samples. An antibody specific for CCL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL2 is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.


I purchased several antibodies form Abbkine in the last months, and have to say this is a trustworthy brand. Recently, I ordered a EliKine™ Human CCL2 ELISA Kit and want to detect the CCL2 in the serum sample. The technical support is very professional and he patiently guide me prepare the samples and calculate the results via the software. Experiments show that Abbkine ELISA kit has high sensitivity and excellent specificity for detection of Human CCL2. No significant cross-reactivity or interference between Human CCL2 and analogues was observed. I really appreciate this experience, I will continue to support Abbkine products.

2017年8月28日星期一

EliKine™ Rat VEGF ELISA Kit review

EliKine™ Rat VEGF ELISA Kit reviewVEGF (Vascular endothelial growth factor) is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is required for regulating the proliferation, migration, and survival of embryonic endothelial cells and during wound healing and the female reproductive cycle in adults. Pathologically, it is involved in tumor development and tumor vascular leakage. VEGF binds to VEGF R1/Flt-1, VEGF R2/Flk-1/KDR, and Neuropilin-1. Multiple forms of VEGF are generated by alternative splicing and proteolysis.


EliKine™ Rat VEGF ELISA Kit employs a two-site sandwich ELISA to quantitate VEGF in samples. An antibody specific for VEGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound in the initial step. The color development is stopped and the intensity of the color is measured.


My group purchased EliKine™ Rat VEGF ELISA Kit to detect VEGF in Rat serum. Compared to other Rat VEGF ELISA Kits in the market, Abbkine kit gives a high performance. The Biotin- Streptavidin amplification system let the whole assay more sensitive. It shows no weird result according to the operation manual. It took about 4h to complete the whole assay. This kit is in line with my expectations. I’ll try Abbkine other products in the near future.

2017年8月25日星期五

A pathology atlas of the human cancer transcriptome

Topics overview: The need for precise and personalized medicine for cancer treatment, Sci-RNA-seq to profile cells, Selective and sustained targeting of endothelial S1P receptors by ApoM-Fc could be a viable therapeutic strategy in vascular diseases, Repurposing existing GLP-1R agonist drugs may be a useful therapeutic strategy for treating raised ICP, VCP inhibition and oncolytic virus as a potential treatment for HCC and demonstrates promising therapeutic potential.


1. A pathology atlas of the human cancer transcriptome


Cancer is one of the leading causes of death, and there is great interest in understanding the underlying molecular mechanisms involved in the pathogenesis and progression of individual tumors. Mathias Uhlen at KTH–Royal Institute of Technology in Stockholm, Sweden and his colleagues used systems-level approaches to analyze the genome-wide transcriptome of the protein-coding genes of 17 major cancer types with respect to clinical outcome. A general pattern emerged: Shorter patient survival was associated with up-regulation of genes involved in cell growth and with down-regulation of genes involved in cellular differentiation. Using genome-scale metabolic models, they show that cancer patients have widespread metabolic heterogeneity, highlighting the need for precise and personalized medicine for cancer treatment. All data are presented in an interactive open-access database (www.proteinatlas.org/pathology) to allow genome-wide exploration of the impact of individual proteins on clinical outcomes.


Read more, please click http://science.sciencemag.org/content/357/6352/eaan2507


2. Comprehensive single-cell transcriptional profiling of a multicellular organism


To resolve cellular heterogeneity, Junyue Cao at University of Washington in WA, USA and his colleagues developed a combinatorial indexing strategy to profile the transcriptomes of single cells or nuclei, termed sci-RNA-seq (single-cell combinatorial indexing RNA sequencing). They applied sci-RNA-seq to profile nearly 50,000 cells from the nematode Caenorhabditis elegans at the L2 larval stage, which provided >50-fold “shotgun” cellular coverage of its somatic cell composition. From these data, they defined consensus expression profiles for 27 cell types and recovered rare neuronal cell types corresponding to as few as one or two cells in the L2 worm. They integrated these profiles with whole-animal chromatin immunoprecipitation sequencing data to deconvolve the cell type–specific effects of transcription factors. The data generated by sci-RNA-seq constitute a powerful resource for nematode biology and foreshadow similar atlases for other organisms.


Read more, please click http://science.sciencemag.org/content/357/6352/661


3. An engineered S1P chaperone attenuates hypertension and ischemic injury


Endothelial dysfunction, a hallmark of vascular disease, is restored by plasma high-density lipoprotein (HDL). However, a generalized increase in HDL abundance is not beneficial, suggesting that specific HDL species mediate protective effects. Apolipoprotein M–containing HDL (ApoM+HDL), which carries the bioactive lipid sphingosine 1-phosphate (S1P), promotes endothelial function by activating G protein–coupled S1P receptors. Moreover, HDL-bound S1P is limiting in several inflammatory, metabolic, and vascular diseases. Steven L. Swendeman at Boston Children’s Hospital in Boston, USA and his colleagues report the development of a soluble carrier for S1P, ApoM-Fc, which activated S1P receptors in a sustained manner and promoted endothelial function. In contrast, ApoM-Fc did not modulate circulating lymphocyte numbers, suggesting that it specifically activated endothelial S1P receptors. ApoM-Fc administration reduced blood pressure in hypertensive mice, attenuated myocardial damage after ischemia/reperfusion injury, and reduced brain infarct volume in the middle cerebral artery occlusion model of stroke. Their proof-of-concept study suggests that selective and sustained targeting of endothelial S1P receptors by ApoM-Fc could be a viable therapeutic strategy in vascular diseases.


Read more, please click http://stke.sciencemag.org/content/10/492/eaal2722


4. A glucagon-like peptide-1 receptor agonist reduces intracranial pressure in a rat model of hydrocephalus


Current therapies for reducing raised intracranial pressure (ICP) under conditions such as idiopathic intracranial hypertension or hydrocephalus have limited efficacy and tolerability. Thus, there is a pressing need to identify alternative drugs. Glucagon-like peptide-1 receptor (GLP-1R) agonists are used to treat diabetes and promote weight loss but have also been shown to affect fluid homeostasis in the kidney. Hannah F. Botfield at Institute of Metabolism and Systems Research, University of Birmingham in Edgbaston, UK and his colleagues investigated whether exendin-4, a GLP-1R agonist, is able to modulate cerebrospinal fluid (CSF) secretion at the choroid plexus and subsequently reduce ICP in rats. They used tissue sections and cell cultures to demonstrate expression of GLP-1R in the choroid plexus and its activation by exendin-4, an effect blocked by the GLP-1R antagonist exendin 9-39. Acute treatment with exendin-4 reduced Na+– and K+-dependent adenosine triphosphatase activity, a key regulator of CSF secretion, in cell cultures. Finally, they demonstrated that administration of exendin-4 to female rats with raised ICP (hydrocephalic) resulted in a GLP-1R–mediated reduction in ICP. These findings suggest that GLP-1R agonists can reduce ICP in rodents. Repurposing existing GLP-1R agonist drugs may be a useful therapeutic strategy for treating raised ICP.


Read more, please click http://stm.sciencemag.org/content/9/404/eaan0972


5. Targeting VCP enhances anticancer activity of oncolytic virus M1 in hepatocellular carcinoma


Oncolytic virotherapy is rapidly progressing through clinical evaluation. However, the therapeutic efficacy of oncolytic viruses in humans has been less than expected from preclinical studies. Haipeng Zhang at Sun Yat-sen University in Guangzhou, China and his colleagues describe an anticancer drug screen for compounds that enhance M1 oncolytic virus activity in hepatocellular carcinoma (HCC). An inhibitor of the valosin-containing protein (VCP) was identified as the top sensitizer, selectively increasing potency of the oncolytic virus up to 3600-fold. Further investigation revealed that VCP inhibitors cooperated with M1 virus–suppressed inositol-requiring enzyme 1α (IRE1α)–X-box binding protein 1 (XBP1) pathway and triggered irresolvable endoplasmic reticulum (ER) stress, subsequently promoting robust apoptosis in HCC. They show that VCP inhibitor improved the oncolytic efficacy of M1 virus in several mouse models of HCC and primary HCC tissues. Finally, this combinatorial therapeutic strategy was well tolerated in nonhuman primates. Their study identifies combined VCP inhibition and oncolytic virus as a potential treatment for HCC and demonstrates promising therapeutic potential.


Read more, please click http://stm.sciencemag.org/content/9/404/eaam7996

2017年8月24日星期四

Anti-mCherry Tag Mouse Monoclonal Antibody (9D3) makes work easier for scientific researchers

Anti-mCherry Tag Mouse Monoclonal Antibody (9D3) makes work easier for scientific researchersThe Anti-mCherry Tag Mouse Monoclonal Antibody (9D3) is the latest effort from scientific research solutions provider, Abbkine Scientific Company Limited. The antibody joins the long list of other such antibodies from the stables of Abbkine Scientific.


Otherwise referred to as mCherry antibody, the product is a fluorescent protein commonly used in biotechnology. The protein is used for different purposes which includes as a tracer for following the flow of fluids, as a marker when tagged to cell components and molecules.


mCherry is derived from an isolated protein and researchers and scientists sometimes preferring it to other fluorophores. This is particularly due to its colour and photostability, compared to its counterparts.


The mCherry Tag antibody is hosted in the mouse with a recombinant protein immunogen. Made exclusively for research purpose, the product is available in a liquid solution and has gone through the best purification process to ensure the best results.


The monoclonal antibody can be applied IF and WB, with the makers suggesting staring dilutions of WB 1:1000-1:10,000, IF 1:100-1:1000. However, researchers are advised to determine the optimal working dilutions experimentally.


If stored at a stable temperature of -20°C, the antibody can remain intact for up to twelve months with the storage buffer being liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative and 50% Glycerol.


About Abbkine Scientific


Abbkine Scientific Co. Ltd is a scientific research company founded by a team of enthusiastic and passionate marketing experts and scientists. Established in 2012 in California, the company moved its headquarters to China due to growing demands from Asia Pacific.


The company combines cutting edge technology from the U.S with the manufacturing power and cost advantages of China to provide innovative scientific solutions and products that include high quality assay kits, recombinant proteins, antibodies for easier and more effective science research.

2017年8月21日星期一

CK17 Monoclonal Antibody review

CK17 Monoclonal Antibody reviewCK17 plays a role in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair. It may be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial “stem cells”. And act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation. CK17 required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway. Involved in tissue repair.


Abbkine CK17 Monoclonal Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The application in WB, IF, IHC-P and IP experiments had been well validated. This antibody can be used to detect endogenous CK17 protein in the sample of Human. According to your own experimental conditions, optimal working dilutions should be explored.


Immunofluorescence analysis of human breast tissue by using Abbkine CK17 Monoclonal Antibody. The antibody was diluted at 1:200. The fluorescence signal produced is strong and the results are accord with my experiment expectation. As a faithful funs of Abbkine, I always trust this brand. This antibody also didn’t let me down. Good specificity, reliable quality and cheaper price, it deserved to be recommended to more researchers.

2017年8月17日星期四

Abbkine announces the launch of Anti-α-Tubulin Monoclonal Antibody (3G5)

Abbkine announces the launch of Anti-α-Tubulin Monoclonal Antibody (3G5)Popular scientific research company, Abbike Scientific, has announced the release of its latest antibody named the Anti-α-Tubulin Monoclonal Antibody (3G5). Otherwise known as TUBA1 antibody, the product joins the illustrious list of scientific tools and products from the research giant.


The alpha tubulin antibody is part of the family of microtubules of the eukaryotic cytoskeleton, with the alpha and beta tubulins being the major components of microtubules. The alpha tubulin genes are conserved among several species.


TUBA1A is monoclonal and very similar to the Tuba1 genes in mouse and rat. Studies have further shown that TUBA1A expression is majorly found in morphologically differentiated neurologic cells. As one of the three alpha-tubulin genes in a cluster on chromosome 12q, the mutation in TUBA1A leads to lissencephaly type 3 (LIS3).


The antibody has a recombinant protein immunogen with human, mouse and rat reactivity. The applications of the antibody include IF, IHC, IP, WB and users are advised to determine the best working dilutions after series of experiments. However, the suggested starting dilutions are WB 1:5000-10000, IP 1:200.


The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen, which is probably one of the unique attributes of the product. The antibody is available in a liquid solution, making it easy for researchers to use, manipulation and loading control.


The makers of the antibody have expressly declared that the product is manufactured for research purpose only and should not be used for human or diagnostic purpose.


About Abbkine Scientific


Abbkine Scientific Co. Ltd is a scientific research company founded by a team of enthusiastic and passionate marketing experts and scientists. Established in 2012 in California, the company moved its headquarters to China due to growing demands from Asia Pacific.


The company combines cutting edge technology from the U.S with the manufacturing power and cost advantages of China to provide innovative scientific solutions and products that include high quality assay kits, recombinant proteins, antibodies for easier and more effective science research.

2017年8月16日星期三

Collagen I Mouse Monoclonal Antibody (4H10) review

Collagen I Mouse Monoclonal Antibody (4H10) reviewCOL1A1(collagen type I alpha 1 chain) encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in COL1A1 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for COL1A1.


Abbkine Collagen I Mouse Monoclonal Antibody (4H10) was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The immunogen of this antibody is Synthetic Peptide of Collagen I. This antibody could be applied to IF and IHC-p experiments. It can react with human, rat and mouse samples. The original concentration of this antibody is 1mg/ml. During the experiment, optimal working dilutions should be explored by the researchers. The suggested starting dilution for IHC-p is 1:50-200.


Two weeks ago, I ordered the Collagen I Mouse Monoclonal Antibody from Abbkine and want to conduct immunofluorescence analysis in rat kidney tissue. This is my first time to use Abbkine antibody, so I just want to try it. Quite unexpectedly, it brings me a good experience. I received the product soon after the order. During the experiment, they also provide careful and professional guidance. Finally, the fluorescence signal produced is strong and the results are accord with my experiment expectation. The antibody has good specificity, reliable quality and the price is cheaper. I want to recommend it to my friends.

2017年8月11日星期五

In vivo CRISPR screening identifies Ptpn2 as a cancer immunotherapy target

Topics overview: Hypothalamic stem cells and ageing speed, The role of AMPA receptors in excitatory neurotransmission, The application of integrative genomics to medulloblastoma, In vivo genetic screens in tumour models, The treatment of blinding retinal diseases.


1. Hypothalamic stem cells control ageing speed partly through exosomal miRNAs


It has been proposed that the hypothalamus helps to control ageing, but the mechanisms responsible remain unclear. Here Yalin Zhang at Albert Einstein College of Medicine in New York, USA and her colleagues develop several mouse models in which hypothalamic stem/progenitor cells that co-express Sox2 and Bmi1 are ablated, as they observed that ageing in mice started with a substantial loss of these hypothalamic cells. Each mouse model consistently displayed acceleration of ageing-like physiological changes or a shortened lifespan. Conversely, ageing retardation and lifespan extension were achieved in mid-aged mice that were locally implanted with healthy hypothalamic stem/progenitor cells that had been genetically engineered to survive in the ageing-related hypothalamic inflammatory microenvironment. Mechanistically, hypothalamic stem/progenitor cells contributed greatly to exosomal microRNAs (miRNAs) in the cerebrospinal fluid, and these exosomal miRNAs declined during ageing, whereas central treatment with healthy hypothalamic stem/progenitor cell-secreted exosomes led to the slowing of ageing. In conclusion, ageing speed is substantially controlled by hypothalamic stem cells, partially through the release of exosomal miRNAs.


Read more, please click http://www.nature.com/nature/journal/vaop/ncurrent/full/nature23282.html


2. Channel opening and gating mechanism in AMPA-subtype glutamate receptors


AMPA-subtype ionotropic glutamate receptors mediate fast excitatory neurotransmission throughout the central nervous system. Gated by the neurotransmitter glutamate, AMPA receptors are critical for synaptic strength and dysregulation of AMPA receptor-mediated signalling is linked to numerous neurological diseases. Here, Edward C. Twomey at Columbia University in New York, USA and her colleagues use cryo-electron microscopy to solve the structures of AMPA receptor-auxiliary subunit complexes in the apo, antagonist and agonist-bound states and elucidate the iris-like mechanism of ion channel opening. The ion channel selectivity filter is formed by the extended portions of the re-entrant M2 loops, while the helical portions of M2 contribute to extensive hydrophobic interfaces between AMPA receptor subunits in the ion channel. They show how the permeation pathway changes upon channel opening and identify conformational changes throughout the entire AMPA receptor that accompany activation and desensitization. Their findings provide a framework for understanding gating across the family of ionotropic glutamate receptors and the role of AMPA receptors in excitatory neurotransmission.


Read more, please click http://www.nature.com/nature/journal/vaap/ncurrent/full/nature23479.html


3. The whole-genome landscape of medulloblastoma subtypes


Current therapies for medulloblastoma, a highly malignant childhood brain tumour, impose debilitating effects on the developing child, and highlight the need for molecularly targeted treatments with reduced toxicity. Previous studies have been unable to identify the full spectrum of driver genes and molecular processes that operate in medulloblastoma subgroups. Here Paul A. Northcott at German Cancer Research Center in Heidelberg, Germany and his colleagues analyse the somatic landscape across 491 sequenced medulloblastoma samples and the molecular heterogeneity among 1,256 epigenetically analysed cases, and identify subgroup-specific driver alterations that include previously undiscovered actionable targets. Driver mutations were confidently assigned to most patients belonging to Group 3 and Group 4 medulloblastoma subgroups, greatly enhancing previous knowledge. New molecular subtypes were differentially enriched for specific driver events, including hotspot in-frame insertions that target KBTBD4 and ‘enhancer hijacking’ events that activate PRDM6. Thus, the application of integrative genomics to an extensive cohort of clinical samples derived from a single childhood cancer entity revealed a series of cancer genes and biologically relevant subtype diversity that represent attractive therapeutic targets for the treatment of patients with medulloblastoma.


Read more, please click http://www.nature.com/nature/journal/v547/n7663/full/nature22973.html


4. In vivo CRISPR screening identifies Ptpn2 as a cancer immunotherapy target


Immunotherapy with PD-1 checkpoint blockade is effective in only a minority of patients with cancer, suggesting that additional treatment strategies are needed. Here Robert T. Manguso at Dana-Farber Cancer Institute in Massachusetts, USA and his colleagues use a pooled in vivo genetic screening approach using CRISPR–Cas9 genome editing in transplantable tumours in mice treated with immunotherapy to discover previously undescribed immunotherapy targets. They tested 2,368 genes expressed by melanoma cells to identify those that synergize with or cause resistance to checkpoint blockade. They recovered the known immune evasion molecules PD-L1 and CD47, and confirmed that defects in interferon-γ signalling caused resistance to immunotherapy. Tumours were sensitized to immunotherapy by deletion of genes involved in several diverse pathways, including NF-κB signalling, antigen presentation and the unfolded protein response. In addition, deletion of the protein tyrosine phosphatase PTPN2 in tumour cells increased the efficacy of immunotherapy by enhancing interferon-γ-mediated effects on antigen presentation and growth suppression. In vivo genetic screens in tumour models can identify new immunotherapy targets in unanticipated pathways.


Read more, please click http://www.nature.com/nature/journal/v547/n7664/full/nature23270.html


5. Stimulation of functional neuronal regeneration from Müller glia in adult mice.


Many retinal diseases lead to the loss of retinal neurons and cause visual impairment. The adult mammalian retina has little capacity for regeneration. By contrast, teleost fish functionally regenerate their retina following injury, and Müller glia (MG) are the source of regenerated neurons. The proneural transcription factor Ascl1 is upregulated in MG after retinal damage in zebrafish and is necessary for regeneration. Although Ascl1 is not expressed in mammalian MG after injury, forced expression of Ascl1 in mouse MG induces a neurogenic state in vitro and in vivo after NMDA (N-methyl-D-aspartate) damage in young mice. However, by postnatal day 16, mouse MG lose neurogenic capacity, despite Ascl1 overexpression. Loss of neurogenic capacity in mature MG is accompanied by reduced chromatin accessibility, suggesting that epigenetic factors limit regeneration. Here Nikolas L. Jorstad at University of Washington in Washington, USA and his colleagues show that MG-specific overexpression of Ascl1, together with a histone deacetylase inhibitor, enables adult mice to generate neurons from MG after retinal injury. The MG-derived neurons express markers of inner retinal neurons, synapse with host retinal neurons, and respond to light. Using an assay for transposase-accessible chromatin with high-throughput sequencing (ATAC–seq), they show that the histone deacetylase inhibitor promotes accessibility at key gene loci in the MG, and allows more effective reprogramming. Their results thus provide a new approach for the treatment of blinding retinal diseases.


Read more, please click http://www.nature.com/nature/journal/vaop/ncurrent/full/nature23283.html


 

2017年8月10日星期四

Abbkine launched Anti-Histone H3 Mouse Monoclonal Antibody (2D10)

Abbkine launched Anti-Histone H3 Mouse Monoclonal Antibody (2D10)The Anti-Histone H3 Mouse Monoclonal Antibody (2D10) is otherwise known as Hist1h3a antibody getting its name from Histone H3, one of the five major histone proteins. Histone H3 is involved in the structuring of chromatin in eukaryotic cells and a major component of nucleosome. Nucleosome wrap and compact DNA limit DNA accessibility to the cellular machineries requiring DNA as a template. Therefore, histones play a pivotal role in the transcription, regulation, DNA replication, repair and chromosomal stability.


The Histone H3 antibody has a human, mouse, rat and yeast reactivity with recombinant protein immunogen and the mouse as host. The monoclonal antibody has Mouse IgG1 isotype with IF, IP, WB applications. The team at Abbkine has suggested starting dilutions of WB 1:2000-5000, IF:1:100-500, IP 1:200, while reiterating that investigators should determine the optimal working dilutions experimentally.


The product is available in liquid solution and has been affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.


The product is made for research purpose only and should be used in human or clinical diagnosis. If stored under the right conditions, the antibody can last for as long as a year.


Abbkine Scientific


Abbkine Scientific has grown from being a relatively small startup that was established in California in 2012 to become a global provider of scientific research products and tools. The company moved it headquarters to China due to growing demands from Asia Pacific.


Established by a team of marketing experts and scientists, Abbkine Scientific Co. Ltd combines state-of-the-art technology from the United States of America with cost advantages and high quality manufacturing from China to provide innovative solutions and products that are designed to enhance research in life sciences.

2017年8月9日星期三

Dedicated IFKine™ antibodies for multiple immunofluorescence labelling

Abbkine IFKine™ are series of specially optimized secondary antibodies with improved brightness, photostability and less nonspecific hybridization and background. Donkey host and other species of serum/IgG absorbed make it ideal choice for fluorescence staining, especially in fluorescence multiple labeling.


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2017年8月7日星期一

ATG7 Mouse Monoclonal Antibody (3D6) Review

ATG7 Mouse Monoclonal Antibody (3D6) ReviewATG7 gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance.


ATG7 Mouse Monoclonal Antibody (3D6) was affinity-purified from mouse ascites by affinity-chromatography using the recombinant protein of ATG7 immunogen. The effects in IF and IHC-P experiments had been well validated. This antibody can be used to detect endogenous ATG7 protein in the sample of Human, Mouse and Rat. According to your own experimental conditions, optimal working dilutions should be explored. The suggested starting dilution for IHC is 1:50-200.


Choosing a good antibody always bring you nice experience. I purchased several products form Abbkine, and have to say this is a trustworthy brand. Abbkine primary antibodies choose synthetic peptides instead of full-length proteins as immunogen, which testifies good results in the research experiments, especially in IF and IHC immunological tests. I conducted immunohistochemical analysis in rat lung tissue by using this antibody, gaining perfect results. The antibody was diluted at 1:200. I really appreciate this experience, I will continue to support Abbkine products.

2017年8月4日星期五

Computational design of trimeric influenza-neutralizing proteins targeting the hemagglutinin receptor binding site

Topics overview: Related analytes associated with physiology and disease, Contiguity preserving transposition” sequencing on beads, Inference of peptidoforms (IPF), Computational design of trimeric influenza-neutralizing proteins targeting the hemagglutinin receptor binding site, A calcium- and light-gated switch to induce gene expression in activated neurons.


1. A wellness study of 108 individuals using personal, dense, dynamic data clouds


Personal data for 108 individuals were collected during a 9-month period, including whole genome sequences; clinical tests, metabolomes, proteomes, and microbiomes at three time points; and daily activity tracking. Using all of these data, Nathan D Price at Institute for Systems Biology in Washington, USA and his colleagues generated a correlation network that revealed communities of related analytes associated with physiology and disease. Connectivity within analyte communities enabled the identification of known and candidate biomarkers (e.g., gamma-glutamyltyrosine was densely interconnected with clinical analytes for cardiometabolic disease). They calculated polygenic scores from genome-wide association studies (GWAS) for 127 traits and diseases, and used these to discover molecular correlates of polygenic risk (e.g., genetic risk for inflammatory bowel disease was negatively correlated with plasma cystine). Finally, behavioral coaching informed by personal data helped participants to improve clinical biomarkers. Their results show that measurement of personal data clouds over time can improve our understanding of health and disease, including early transitions to disease states.


Read more, please click http://www.nature.com/nbt/journal/vaop/ncurrent/full/nbt.3870.html


2. Haplotype phasing of whole human genomes using bead-based barcode partitioning in a single tube


Haplotype-resolved genome sequencing promises to unlock a wealth of information in population and medical genetics. However, for the vast majority of genomes sequenced to date, haplotypes have not been determined because of cumbersome haplotyping workflows that require fractions of the genome to be sequenced in a large number of compartments. Here Fan Zhang at Advanced Research Department, Illumina in San Diego, California, USA and his colleagues demonstrate barcode partitioning of long DNA molecules in a single compartment using “on-bead” barcoded tagmentation. The key to the method that they call “contiguity preserving transposition” sequencing on beads (CPTv2-seq) is transposon-mediated transfer of homogenous populations of barcodes from beads to individual long DNA molecules that get fragmented at the same time (tagmentation). These are then processed to sequencing libraries wherein all sequencing reads originating from each long DNA molecule share a common barcode. Single-tube, bulk processing of long DNA molecules with ~150,000 different barcoded bead types provides a barcode-linked read structure that reveals long-range molecular contiguity. This technology provides a simple, rapid, plate-scalable and automatable route to accurate, haplotype-resolved sequencing, and phasing of structural variants of the genome.


Read more, please click http://www.nature.com/nbt/journal/vaop/ncurrent/full/nbt.3897.html


3. Inference and quantification of peptidoforms in large sample cohorts by SWATH-MS


Consistent detection and quantification of protein post-translational modifications (PTMs) across sample cohorts is a prerequisite for functional analysis of biological processes. Data-independent acquisition (DIA) is a bottom-up mass spectrometry approach that provides complete information on precursor and fragment ions. However, owing to the convoluted structure of DIA data sets, confident, systematic identification and quantification of peptidoforms has remained challenging. Here, George Rosenberger at Department of Biology, Institute of Molecular Systems Biology, ETH Zurich in Zurich, Switzerland and his colleagues present inference of peptidoforms (IPF), a fully automated algorithm that uses spectral libraries to query, validate and quantify peptidoforms in DIA data sets. The method was developed on data acquired by the DIA method SWATH-MS and benchmarked using a synthetic phosphopeptide reference data set and phosphopeptide-enriched samples. IPF reduced false site-localization by more than sevenfold compared with previous approaches, while recovering 85.4% of the true signals. Using IPF, they quantified peptidoforms in DIA data acquired from >200 samples of blood plasma of a human twin cohort and assessed the contribution of heritable, environmental and longitudinal effects on their PTMs.


Read more, please click http://www.nature.com/nbt/journal/vaop/ncurrent/full/nbt.3908.html


4. Computational design of trimeric influenza-neutralizing proteins targeting the hemagglutinin receptor binding site


Many viral surface glycoproteins and cell surface receptors are homo-oligomers and thus can potentially be targeted by geometrically matched homo-oligomers that engage all subunits simultaneously to attain high avidity and/or lock subunits together. The adaptive immune system cannot generally employ this strategy since the individual antibody binding sites are not arranged with appropriate geometry to simultaneously engage multiple sites in a single target homo-oligomer. Eva-Maria Strauch at Department of Biochemistry, University of Washington in Washington, USA and her colleagues describe a general strategy for the computational design of homo-oligomeric protein assemblies with binding functionality precisely matched to homo-oligomeric target sites. In the first step, a small protein is designed that binds a single site on the target. In the second step, the designed protein is assembled into a homo-oligomer such that the designed binding sites are aligned with the target sites. They use this approach to design high-avidity trimeric proteins that bind influenza A hemagglutinin (HA) at its conserved receptor binding site. The designed trimers can both capture and detect HA in a paper-based diagnostic format, neutralizes influenza in cell culture, and completely protects mice when given as a single dose 24 h before or after challenge with influenza.


Read more, please click http://www.nature.com/nbt/journal/v35/n7/full/nbt.3907.html


5. A calcium- and light-gated switch to induce gene expression in activated neurons.


Despite recent advances in optogenetics, it remains challenging to manipulate gene expression in specific populations of neurons. Dongmin Lee at Max Planck Florida Institute for Neuroscience in Florida, USA and her colleagues present a dual-protein switch system, Cal-Light, that translates neuronal-activity-mediated calcium signaling into gene expression in a light-dependent manner. In cultured neurons and brain slices, they show that Cal-Light drives expression of the reporter EGFP with high spatiotemporal resolution only in the presence of both blue light and calcium. Delivery of the Cal-Light components to the motor cortex of mice by viral vectors labels a subset of excitatory and inhibitory neurons related to learned lever-pressing behavior. By using Cal-Light to drive expression of the inhibitory receptor halorhodopsin (eNpHR), which responds to yellow light, they temporarily inhibit the lever-pressing behavior, confirming that the labeled neurons mediate the behavior. Thus, Cal-Light enables dissection of neural circuits underlying complex mammalian behaviors with high spatiotemporal precision.


Read more, please click http://www.nature.com/nbt/journal/vaop/ncurrent/full/nbt.3902.html

2017年8月3日星期四

Abbkine Scientific launches new resin - PurKine™ Protein A/G Resin 4FF

PurKine™ Protein AG Resin 4FFPurKine™ Protein A/G Resin 4FF is the latest addition to the Abbkine resin family. The product otherwise known as Protein A/G Resin is designed to allow for effective and easy purification of IgG from serum, ascites fluid, cell culture supernantant and other such antibody samples.


The chemically modified Recombinant Protein A/G helps to reduce nonspecific binding of proteins, allowing researchers and investigators to get their desired results easily. It also binds perfectly well to all human IgG subclasses as well as all mouse IgG subclasses.


The product has reported to be different from its peers due to the flexbility of use, as it is available in multiple formats including bulk resin, spin columns and complete kits. The Protein A/G Agarose is also available as pre-packed spin column and kit formats. Studies have revealed the cost-effectiveness of the product, with no decrease in performance after up to five repeated uses.


The product has been deliberately made to be used in IgG purification applications in gravity column procedures at a variety of scales. Available in liquid solution with 50% slurry in 20% ethanol, the resin is robust as its highly cross-linked beads tolerate linear flow rates up to 300cm/hour.


About Abbkine Scientific Co. Ltd


Abbkine Scientific Company Limited is a research company poised with making scientific research easy and more effective by ensuring the supply of high quality and innovative research tools for the advancement of human and animal health.


Originally established in California by a group of scientists and marketing experts, the company moved its headquarters to China in a bid to meet the growing demands from Asia Pacific. It has subsequently manufactured innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to aid the acceleration of life science fundamental research, drug discovery.