CK16 Monoclonal Antibody Review

Keratin 16 is a protein that in human is encoded by the KRT16 gene. Keratin 16 is a type I cytokeratin. It is paired with keratin 6 in a number of epithelial tissues, including nail bed, esophagus, tongue, and hair follicles. Mutations in the gene encoding this protein are associated with the genetic skin disorders pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus.

Abbkine CK16 Monoclonal Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody detects endogenous levels of total Cytokeratin 16 protein. It reacts with  Human, Mouse and Rat. Test confirmed this antibody was suitable for IF and IHC-p. Abbkine suggested starting dilutions are as follows: IF: 1:200, IHC-p: 1:200. The isotype is Mouse IgG1.

Abbkine CK16 Monoclonal Antibody stained Rat liver tissue sections. The tissue sections were then incubated with the antibody (ABM40056, diluted at 1:200) overnight at 4°C. IFKine™ Orange Donkey Anti-Mouse IgG was used as secondary antibody at a 1/1000 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (blue). The signal produced was very strong and clear. The sensitivity of the antibody was also perfect.