2020年4月16日星期四

New product: IL-6, TNF-alpha, bFGF, IFN-gamma recombinant protein upgrading

The new product line in this recombinant protein series:





Human
IL-6 protein
:Interleukin 6 (IL-6) is a pleiotropic
cytokine that plays an important role in host defense by regulating immune and
inflammatory responses. IL-6 is an important member of the cytokine network and
is central to the acute inflammatory response. After the production of IL-6, it
induces the production of C-reactive protein (CRP) and procalcitonin (PCT),
which are associated with inflammatory diseases and infections. The degree is
directly related. IL-6 can quickly diagnose early inflammation and warn of
sepsis.





Human
TNF-alpha protein
:Tumor necrosis factor-α (TNF-α) is a cytokine
involved in systemic inflammation, mainly secreted by macrophages. Its main function
is to regulate immune cells. As an endogenous pyrogen, it can cause fever,
cause apoptosis, prevent tumorigenesis and virus replication. Its dysfunction
is thought to be related to many diseases, such as Alzheimer's disease,
psoriasis, cancer, major depression, and enteritis. TNF-a is an important part
of the cell signaling pathway, so it has become the target of multiple drugs.





Human
bFGF protein
、Mouse bFGF protein:





Basic fibroblast growth factor (bFGF) is a trace
substance present in mammals and a multifunctional cell growth factor. bFGF has
obvious tendency activity to wound cells, induces inflammatory cells,
fibroblasts, vascular endothelial cells to move to the wound site, activates
the phagocytic function of macrophages, improves the body's immune activity,
and significantly reduces the chance of wound infection.





Human
IFN-gamma protein, His Tag
、Mouse IFN-gamma protein, His Tag:IFN-gamma belongs
to the type II interferon family, which is
widely involved in immune and inflammatory responses. Proper activation of IFN-γ signaling pathway can promote the activation
of macrophages and mediate the host's defense function against pathogens. At
the same time, IFN-γ signaling
pathway can also play an immunomodulatory role in anti-tumor immunity.





New product details:





Product supply form: freeze-dried powder
form, very stable at room temperature. Recommendation: Lyophilized protein
product should be stored desiccated below -20°C.. After dissolution, the
product can be stored at 4℃ for 2-7 days, please keep it below -20℃ for long-term
use. For long-term storage, it is recommended to add carrier protein (0.1% HSA
or BSA), and store it separately as appropriate to avoid repeated freezing and
thawing.





Application areas: antibody preparation or
detection, ELISA standards, cell or tissue culture and other functional
studies.





Price guarantee: better discounts, lower prices.






Product name

CAT#

Preparation method

Purity

Formulation

Size/ Price
 

Fig. SDS-PAGE

Human IL-6 protein
 

PRP1012
 

E. coli
 
> 95 %  
Lyophilized powder

5ug/$48
20ug/$120
100ug/$300
1mg/$800
 

Human TNF-alpha protein
 

PRP1013
 

E. coli
 
> 95 %   Lyophilized powder
10ug/$48
50ug/$120
100ug/$170
1mg/$800
 

Human bFGF protein
 

PRP1010
 

E. coli
 

> 95 %
 

Lyophilized powder

10ug/$48
50ug/$120
100ug/$170
1mg/$900

Mouse bFGF protein
 

PRP1011
 

E. coli
 

> 95 %
 

Lyophilized powder

10ug/$48
50ug/$120
100ug/$170
1mg/$900

Human IFN-gamma protein, His
Tag

PRP1014
 

E. coli
 

> 95 %
 

Lyophilized powder

20ug/$48
100ug/$120
500ug/$450
1mg/$800

Mouse IFN-gamma protein, His
Tag

PRP1015
 

E. coli
 

> 95 %
 

Lyophilized powder

20ug/$48
100ug/$120
500ug/$450
1mg/$800
 

Abbkine focuses on the fields of immunology
and cytology, and is committed to innovating and developing various types of
antibodies, proteins, analytical reagents and kits, with a view to becoming a
key promoter in the fields of life science research and development, and drug
development. Here, we present to you the favorite products of protein and
immune research users, from basic immunological products, such as protein
extraction and quantification, to internal reference label antibodies, primary
and secondary antibodies for immunological experiments; cell research users’
favorite Products ranging from dyes and kits for cell state detection,
organelle extraction kits, cell substructure staining and cell metabolism
detection products, to cytokine and protein detection kits for cell culture,
just to help you Research career!





About Abbkine Scientific Co., Ltd.





Abbkine Scientific Co., Ltd. was founded in
2012. Our mission is to become a respected and world-class supplier of
biomedical products and services. Through a clear core strategy and a corporate culture that
promotes learning, innovation, cooperation, and excellence, we will stimulate
our inherent creativity, provide competitive biomedical products and services,
and continue to create maximum value for customers and achieve our mission.

2020年4月1日星期三

How to choose appropriate Catalase (CAT) Activity Assay Kit?

Catalase (CAT) is an antioxidant enzyme commonly found in almost all living organisms. Its main function is to catalyze the decomposition of hydrogen peroxide into water and oxygen and remove hydrogen peroxide in the body, thus preventing cells from being poisoned by H2O2. It is one of the key enzymes in the biological defense system and provides an antioxidant defense mechanism for the body.





In this article, for CAT Activity Assay Kit, the author chose different brands (Cayman Chemical, Abcam, Biovision) and compared them with Abbkine CheKine™ Catalase (CAT) Activity Assay Kit (Cat#: KTB1040) from several aspects such as assay principle, detection, sample type and size/price.





Summary: For CAT Activity Assay Kit, the brand Abbkine and Cayman Chemical are the similar in Assay Principle. Methanol is taken as a reaction substrate. While Abcam and Biovision take H2O2 as a reaction substrate.





But H2O2 is not only a single substrate of catalase (CAT), other peroxidases in the sample may interfere with the experimental results, and the standard curve error may be caused by instability of hydrogen peroxide. While methanol is the unique substrate of catalase, other peroxidases cannot use methanol as substrate, so interference signals are avoided.





Details of different brands of products are as follows,





BrandAssay PrincipleSample typeSize/Price
AbbkineThis assay kit utilizes the peroxidatic function of catalase for measuring catalase activity, based on the reaction of catalase with methanol, with the presence of an optimal concentration of H2O2. The formaldehyde produced can be measured colorimetrically at OD 540 nm. Therefore, the catalase activity present in the sample is proportional to the signal obtained.Tissue Homogenate, Cell Lysate, serum, plasma, erythrocyte lysate48T/$40
96T/$60
480T/$180
Cayman ChemicalThis assay kit utilizes the peroxidatic function of catalase for measuring catalase activity, based on the reaction of catalase with methanol, with the presence of an optimal concentration of H2O2. The formaldehyde produced can be measured colorimetrically at OD 540 nm. Therefore, the catalase activity present in the sample is proportional to the signal obtained.Tissue Homogenate, Cell Lysate, serum, plasma, erythrocyte lysate96T/$270

480T/$490
AbcamThe catalase present in the sample reacts with hydrogen peroxide (H2O2) to produce water and oxygen. The unconverted H2O2 reacts with probe to produce a product that can be measured colorimetrically at OD 570 nm.Serum, Plasma, Cell Lysate, Tissue Lysate, Urine100T/$415
BiovisionThe catalase present in the sample reacts with hydrogen peroxide (H2O2) to produce water and oxygen. The unconverted H2O2 reacts with probe to produce a product that can be measured colorimetrically at OD 570 nm.Tissue Homogenate, Cell Lysate, erythrocyte lysate100T/$340

About Abbkine Scientific Co., Ltd.





Abbkine serves global scientists in the field of proteomics and cytology and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, expecting to accelerate the pace of life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, with ultimate aim to facilitate your research career.

2020年3月13日星期五

How to choose appropriate NADP/NADPH Assay Kit?

NADP (coenzyme II) is the coenzyme of many redox reactions, including NADP+ (oxidation type) and NADPH (reduction type). NADP+ is also involved in biosynthesis reactions, such as lipid and nucleic acid synthesis. In animal cells, the oxidation stage of pentose phosphate pathway (PPP) is the main source of NADPH. 





In this article, for NADP/NADPH Assay Kit, the author chose different brands (BioAssay Systems, Abcam, Biovision) and compared them with Abbkine CheKine™NADP/NADPH Assay Kit (Cat#: KTB1010) from several aspects such as assay principle, detection, sample type and size/price.





Summary:





These brands are the similar in Assay Principle, Detection and Sample type. Prices are different. The price of Abbkine NADP/NADPH Assay Kit (Cat#: KTB1010) is the lowest.





Besides good price, Abbkine NADP/NADPH Assay Kit (Cat#: KTB1010) has other advantages. The detection limit of Abbkine NADP/NADPH Assay Kit is 0.1 µM and linearity up to 4 µM NADP+/NADPH in 96-well plate assay.





Fig. Standard Curve of NADPH in 96-well plate assay. The y-axis is ΔOD and the x-axis is NADPH concentration (uM).

Details of different brands for NADP/NADPH Assay Kit are as follows,





BrandAssay PrincipleDetectionSample typeSize/Price
AbbkineThe assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 565 nm. Detects NADP+, NADPH, or total NADP+/NADPH Tissue Extracts, Cell Lysate48T/$90
96T/$150
BioAssay SystemsThe assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 565 nm. Detects NADP+, NADPH, or total NADP+/NADPH Tissue Extracts, Cell Lysate100T/$379
AbcamThe assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 450 nm. Detects NADP+, NADPH, or total NADP+/NADPH Tissue Extracts, Cell Lysate100T/$615
BiovisionThe assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 450 nm. Detects NADP+, NADPH, or total NADP+/NADPH Tissue Extracts, Cell Lysate100T/$535




About Abbkine Scientific Co., Ltd.





Abbkine serves global scientists in the field of proteomics and cytology and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, expecting to accelerate the pace of life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, with ultimate aim to facilitate your research career.

How to choose appropriate NADP/NADPH Assay Kit?

NADP (coenzyme II) is the coenzyme of many redox reactions, including NADP+ (oxidation type) and NADPH (reduction type). NADP+ is also involved in biosynthesis reactions, such as lipid and nucleic acid synthesis. In animal cells, the oxidation stage of pentose phosphate pathway (PPP) is the main source of NADPH. 





In this article, for NADP/NADPH Assay Kit, the author chose different brands (BioAssay Systems, Abcam, Biovision) and compared them with Abbkine CheKine™NADP/NADPH Assay Kit (Cat#: KTB1010) from several aspects such as assay principle, detection, sample type and size/price.





Summary:





These brands are the similar in Assay Principle, Detection and Sample type. Prices are different. The price of Abbkine NADP/NADPH Assay Kit (Cat#: KTB1010) is the lowest.





Besides good price, Abbkine NADP/NADPH Assay Kit (Cat#: KTB1010) has other advantages. The detection limit of Abbkine NADP/NADPH Assay Kit is 0.1 µM and linearity up to 4 µM NADP+/NADPH in 96-well plate assay.





Fig. Standard Curve of NADPH in 96-well plate assay. The y-axis is ΔOD and the x-axis is NADPH concentration (uM).

Details of different brands for NADP/NADPH Assay Kit are as follows,





BrandAssay PrincipleDetectionSample typeSize/Price
AbbkineThe assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 565 nm. Detects NADP+, NADPH, or total NADP+/NADPH Tissue Extracts, Cell Lysate48T/$90
96T/$150
BioAssay SystemsThe assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 565 nm. Detects NADP+, NADPH, or total NADP+/NADPH Tissue Extracts, Cell Lysate100T/$379
AbcamThe assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 450 nm. Detects NADP+, NADPH, or total NADP+/NADPH Tissue Extracts, Cell Lysate100T/$615
BiovisionThe assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 450 nm. Detects NADP+, NADPH, or total NADP+/NADPH Tissue Extracts, Cell Lysate100T/$535




About Abbkine Scientific Co., Ltd.





Abbkine serves global scientists in the field of proteomics and cytology and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, expecting to accelerate the pace of life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, with ultimate aim to facilitate your research career.

How to choose appropriate NADP/NADPH Assay Kit?

NADP (coenzyme II) is the coenzyme of many redox reactions, including NADP+ (oxidation type) and NADPH (reduction type). NADP+ is also involved in biosynthesis reactions, such as lipid and nucleic acid synthesis. In animal cells, the oxidation stage of pentose phosphate pathway (PPP) is the main source of NADPH. 





In this article, for NADP/NADPH Assay Kit, the author chose different brands (BioAssay Systems, Abcam, Biovision) and compared them with Abbkine CheKine™NADP/NADPH Assay Kit (Cat#: KTB1010) from several aspects such as assay principle, detection, sample type and size/price.





Summary:





These brands are the similar in Assay Principle, Detection and Sample type. Prices are different. The price of Abbkine NADP/NADPH Assay Kit (Cat#: KTB1010) is the lowest.





Besides good price, Abbkine NADP/NADPH Assay Kit (Cat#: KTB1010) has other advantages. The detection limit of Abbkine NADP/NADPH Assay Kit is 0.1 µM and linearity up to 4 µM NADP+/NADPH in 96-well plate assay.





Fig. Standard Curve of NADPH in 96-well plate assay. The y-axis is ΔOD and the x-axis is NADPH concentration (uM).

Details of different brands for NADP/NADPH Assay Kit are as follows,





BrandAssay PrincipleDetectionSample typeSize/Price
AbbkineThe assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 565 nm. Detects NADP+, NADPH, or total NADP+/NADPH Tissue Extracts, Cell Lysate48T/$90
96T/$150
BioAssay SystemsThe assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 565 nm. Detects NADP+, NADPH, or total NADP+/NADPH Tissue Extracts, Cell Lysate100T/$379
AbcamThe assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 450 nm. Detects NADP+, NADPH, or total NADP+/NADPH Tissue Extracts, Cell Lysate100T/$615
BiovisionThe assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 450 nm. Detects NADP+, NADPH, or total NADP+/NADPH Tissue Extracts, Cell Lysate100T/$535




About Abbkine Scientific Co., Ltd.





Abbkine serves global scientists in the field of proteomics and cytology and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, expecting to accelerate the pace of life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, with ultimate aim to facilitate your research career.

How to choose appropriate NADP/NADPH Assay Kit?

NADP (coenzyme II) is the coenzyme of many redox reactions, including NADP+ (oxidation type) and NADPH (reduction type). NADP+ is also involved in biosynthesis reactions, such as lipid and nucleic acid synthesis. In animal cells, the oxidation stage of pentose phosphate pathway (PPP) is the main source of NADPH. 





In this article, for NADP/NADPH Assay Kit, the author chose different brands (BioAssay Systems, Abcam, Biovision) and compared them with Abbkine CheKine™NADP/NADPH Assay Kit (Cat#: KTB1010) from several aspects such as assay principle, detection, sample type and size/price.





Summary:





These brands are the similar in Assay Principle, Detection and Sample type. Prices are different. The price of Abbkine NADP/NADPH Assay Kit (Cat#: KTB1010) is the lowest.





Besides good price, Abbkine NADP/NADPH Assay Kit (Cat#: KTB1010) has other advantages. The detection limit of Abbkine NADP/NADPH Assay Kit is 0.1 µM and linearity up to 4 µM NADP+/NADPH in 96-well plate assay.





Fig. Standard Curve of NADPH in 96-well plate assay. The y-axis is ΔOD and the x-axis is NADPH concentration (uM).

Details of different brands for NADP/NADPH Assay Kit are as follows,





BrandAssay PrincipleDetectionSample typeSize/Price
AbbkineThe assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 565 nm. Detects NADP+, NADPH, or total NADP+/NADPH Tissue Extracts, Cell Lysate48T/$90
96T/$150
BioAssay SystemsThe assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 565 nm. Detects NADP+, NADPH, or total NADP+/NADPH Tissue Extracts, Cell Lysate100T/$379
AbcamThe assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 450 nm. Detects NADP+, NADPH, or total NADP+/NADPH Tissue Extracts, Cell Lysate100T/$615
BiovisionThe assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 450 nm. Detects NADP+, NADPH, or total NADP+/NADPH Tissue Extracts, Cell Lysate100T/$535




About Abbkine Scientific Co., Ltd.





Abbkine serves global scientists in the field of proteomics and cytology and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, expecting to accelerate the pace of life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, with ultimate aim to facilitate your research career.

2020年2月28日星期五

The Statement of Strengthening Epidemic Prevention for COVID-19

Dear Colleagues,





The situation for COVID-19’s outbreak in Wuhan is getting improved in the strong supports of our government and caring people all over the world now. We are very lucky that we all are safe at present, while it’s still very important for us to do well protections. We determine to do below provisional measures during the resumption of production until the end of the outbreak in order to ensure the safe of all colleagues and our clients strictly.





  1. The establishment of epidemic prevention group, their works contain procurement of protection materials, temperature detection, virus kill, daily patrol and consults ect;
  2. Purchasing a batch of substance such as medicine alcohol, chlorine disinfectant, medical surgical masks, protective clothing, temperature guns for epidemic prevention;
  3. The offices, production laboratories, warehouses must be disinfected fully after daily work, and all windows must be opened;
  4. The temperature detection is needed before and after daily work for everyone, any person has the temperature over 37.3℃ must be sent to the hospital immediately. Early detection, early isolation, early treatment;
  5. All colleagues must wear mask and the colleagues in production department also must wear protective clothing, gloves, wash and change them frequently;
  6. The new purchased materials must be kept in isolated room first, and transferred into warehouses or production rooms after being thoroughly sprayed and disinfected with 75% medicine alcohol;
  7. The high temperature resistant consumables, such as bottle, pipe, experimental spear, must be sterilized via high heat and pressure, and those consumables don’t resistant to high temperature, such as box, bag, must be thoroughly sprayed and disinfected with 75% medicine alcohol to ensure the safe of products during the production;
  8. During the process of packing box, the inner and outer surface of the box must be sprayed and disinfected with 75% medicine alcohol to ensure the packaging safety;
  9. Cargos should be transported via reliable express company such as S.F. Express, FedEx, DHL to ensure the transportation safety;

Abbkine Scientific Co.,Ltd.





20th, February 2020