MBP-Tag Protein Purification Kit – a new high performance product from Abbkine

Wuhan, China. 430074. 27^th February 2017. PurKine™ MBP-Tag Protein Purification Kit (Dextrin), otherwise known as MBP resin or Dextrin resin is another purification kit from world leading Chinese scientific company, Abbkine Scientific. PurKine™ MBP-Tag Purification system is a product of innovative high-capacity matrix made for the easy purification of recombinant maltose binding proteins - MBP-tagged protein, giving it its other name, MBP tag resin.

PurKine™ MBP-Tag Protein Purification Kit comprises of PurKine™ MBP-Tag Dextrin Resin 6FF, Binding/Wash buffer (10X) and Elution buffer (10x). The uniqueness of the kit comes from the ingenuity in its production. As opposed to the traditional method of producing resin, the MBP tag resin is made using the latest synthetics process and state-of-the-art technique to develop the resin and optimize the accessories.

The cost effective purification kit comes with other benefits such as high capacity of more than 20 mg of MBP-tagged protein per millilitre of resin, high performance with the preservation of target protein activity, cost-effectiveness that stems from retained level of performance after repeated use, ease of use, and flexibility as it is available in different formats like bulk resin, spin columns and complete kits.

Dextrin is to be shipped in blue ice and should be stored at a stable temperature of between 2°C and 8°C for one year from the date it was shipped.

 About Abbkine Scientific

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California, USA in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.

NFκB-p65 (Acetyl Lys310) Polyclonal Antibody Review

Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses . NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. Acetylation at Lys-310 is required for full transcriptional activity in the absence of effects on DNA binding and NFKBIA association. Acetylation can also lower DNA-binding and results in nuclear export.

Abbkine NFκB-p65 (Acetyl Lys310) Polyclonal Antibody detects endogenous levels of NFκB-p65 protein only when acetylated at Lys310 in Human and Mouse samples. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using synthesized peptide derived from human NFκB-p65 around the acetylation site of K310. at AA rangle: 250-330. It works for WB, IHC-p, IF and ELISA.

I chose Abbkine NFκB-p65 (Acetyl Lys310) Polyclonal Antibody to stain NF-κB p65 murine peritoneal tumour cells by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 60 minutes at room temperature. Samples were incubated with primary antibody (1/1000) for 2 hours. IFKine^TM Green Donkey Anti-Rabbit IgG was used as the secondary antibody. The target was stained and no background. In a word, everything goes well.

Histone H4 (Acetyl Lys16) Polyclonal Antibody Review

The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Histone acetylation occurs mainly on the amino-terminal tail domains of histones H2A (Lys5), H2B (Lys5, 12, 15, and 20), H3 (Lys9, 14, 18, 23, 27, 36 and 56), and H4 (Lys5, 8, 12, and 16) and is important for the regulation of histone deposition, transcriptional activation, DNA replication, recombination, and DNA repair.

Abbkine Histone H4 (Acetyl Lys16) Polyclonal Antibody recognizes endogenous levels of histone H4 protein only when acetylated at Lys16 in Human, Mouse and Rat samples. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using synthesized peptide derived from human Histone H4 around the acetylation site of K16. It works for WB, IHC-p, IF and ELISA.

Western Blot analysis of KB cells using Abbkine Histone H4 (Acetyl Lys16) Polyclonal Antibody at 1:1000 dilution. Secondary antibody (catalog#：A21020) was diluted at 1:20000. I observed the band size 11 kDa which is the same as predicted. Whether the price or the performance, this antibody is just what I want.

Histone H3 (Acetyl Lys9) Polyclonal Antibody Review

The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression. Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells.  In most species, Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms.

Abbkine Histone H3 (Acetyl Lys9) Polyclonal Antibody detects endogenous levels of Histone H3 only when acetylated at lysine 9 in Human, Mouse and Rat samples. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using synthesized peptide derived from human Histone H3 around the acetylation site of K9. at AA range: 1-80. It works for WB, IHC-p, IF and ELISA.

My sample is mouse embryonic fibroblasts and I purchased Abbkine Histone H3 (Acetyl Lys9) Polyclonal Antibody for WB. The antibody works well at the recommended concentration 1:2000.  The secondary antibody I chose was also from Abbkine(CAT#A21020). This is my first time using Abbkine’s products, and I think Abkkine will be a perfect supplier in my future research life.

Abbkine Scientific announces the launch of PurKine™ GST-Tag Protein Purification Kit (Glutathione)

Abbkine Scientific Company Limited recently announced the launch of PurKine™ GST-Tag Protein Purification Kit (Glutathione), otherwise known as Glutathione or GST resin, a new product from the renowned research company.

Glutathione resin kit as the product is also known is a purification kit that is made specifically to purify high levels of overexpressed GST-tagged fusion proteins in gravity column procedure at different scales.

Consisting of 90μm beads of cross-linked 4% agarose, GST tag resin has gone under examination with other famous GST-Tag purification resins, with the former performing at par and sometimes exceeding its peers. The product is even more unique as the performance of the PurKine™ GST-Tag Protein Purification Kit remained untampered even after repeated uses.

The kit is designed to make the workflow easier, more affordable and more effective. The product is a series of unique purification package of settled resin, prepacked spin columns, and full kits for any sample preparation and purification.

Abbkine in the production of the kit deviated from the tradition, using the latest synthetics process in combination with the latest techniques for the development of resin and optimization of accessories. The kit comprises of PurKine™ GST-Tag Glutathione Packed Column, Binding/Wash Buffer (10X) and Elution buffer (10x).

The kit comes with unique features and benefits, which include high performance, high capacity, cost-effectiveness, flexibility and ease of use. The kit can be stored at a stable temperature of 2-8°C for a year from the shipment date, with blue ice shipping.

About Abbkine Scientific

Abbkine Scientific Company Limited is a life science research company. Founded in 2012, the establishment has been able to spread its tentacles across the globe with increasing presence and acceptance from Asia Pacific thanks to its continuous efforts to make the world a better place.

Abbkine combines cutting edge technology with manufacturing engineering and cost advantage to provide innovative, high-quality assay kits and other research and scientific products enhance life science fundamental research and drug discovery amongst others.

RANKL Polyclonal Antibody Review

RANKL is known as a type II membrane protein and is a member of the tumor necrosis factor (TNF) superfamily. RANKL has been identified to affect the immune system and control bone regeneration and remodeling. RANKL is an apoptosis regulator gene, a binding partner of osteoprotegerin (OPG), a ligand for the receptor RANK and controls cell proliferation by modifying protein levels of Id4, Id2 and cyclin D1. RANKL is expressed in several tissues and organs including: skeletal muscle, thymus, liver, colon, small intestine, adrenal gland, osteoblast, mammary gland epithelial cells, prostate and pancreas. Variation in concentration levels of RANKL throughout several organs, reconfirm the uses and importance of growth (particularly bone growth) and immune functions within the body.

RANKL Polyclonal Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been tested with WB, IHC-p and ELISA. And Abbkine suggested starting dilutions are as follows:WB: 1:500-1:2000, IHC-p: 1:100-1:300, ELISA: 1:20000.

RANKL is a member of the tumor necrosis factor (TNF) cytokine family, it binds to RANK on cells of the myeloid lineage and functions as a key factor for osteoclast differentiation and activation. To my knowledge, Abbkine is a great option when you want to find a cost-effective product. It is worth recommending.

Cleaved-PARP-1 (D214) Polyclonal Antibody Review

Poly [ADP-ribose] polymerase 1 (PARP-1) also known as NAD+ ADP-ribosyltransferase 1 or poly[ADP-ribose] synthase 1 is an enzyme that in humans is encoded by the PARP1 gene. It is one of the PARP family of enzymes. PARP-1 involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks Mediates the poly(ADP-ribosyl)ation of APLF and CHFR .

Abbkine Cleaved-PARP-1 (D214) Polyclonal Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using synthesized peptide derived from the Internal region of human PARP-1. at AA rangle: 140-220. The antibody  was tested to apply in ELISA and WB with Human and Mouse samples. The suggested starting dilutions are: WB: 1:500-1:2000, ELISA: 1:20000.

The Cleaved-PARP-1(D214) Polyclonal antibodie is performing very well for our research clinical trials. We used it on mouse osteosarcoma cells for clinical trials to verify results that we had gotten from an antibody from another manufacturer. The PARP-1 (D214) worked even better. It was able to detect PARP-1 . We first tried the recommended WB 1:500 concentration and it worked great. The signal produced was very strong and clear.  The sensitivity of the PARP-1 (D214) was also perfect. Apart from the performance of the antibody, it was also great to be able to order the antibody in trial-sized quantities.