【Tips】If you are studying whether a certain drug can trigger apoptosis which relys on the caspase related pathway, and want to detect the activity of caspase, this article is suitable for you! This article is especially fit for researchers who are working hard to benefit the mankind.
【Background】
Caspases(cysteine-aspartic proteases) are a
family of protease enzymes playing essential roles in programmed cell death
(including apoptosis, pyroptosis and necroptosis) and inflammation. The cascade
reaction caused by caspases is a central part of the apoptotic process, and its
activation mainly includes the mitochondria-dependent pathway and the death
receptor-mediated signal transduction pathway. The downstream caspases are then
activated, leading to apoptosis by cleavage of specific substrates. Inhibition
of apoptotic function will lead to tumorigenesis and abnormal immune function.
However, hyperfunction of apoptotic function is closely related to the
pathogenesis of tissue damage and organ failure.
【Caspase Structure】
【Assay Principle】
Abbkine Caspase Assay Kits are based on different
caspases can catalyze specific substrates to generate pNA(p-nitroaniline), resulting
in the release of p-nitroaniline (pNA), which can be quantified using a
spectrophotometer or a microtiter plate reader at at 405 nm.
Product name | Cat# |
Caspase-1 Assay Kit (Colorimetric) | KTA3020 |
Caspase-2 Assay Kit (Colorimetric) | KTA3021 |
Caspase-3 Assay Kit (Colorimetric) | KTA3022 |
Caspase-4 Assay Kit (Colorimetric) | KTA3023 |
Caspase-6 Assay Kit (Colorimetric) | KTA3024 |
Caspase-8 Assay Kit (Colorimetric) | KTA3025 |
Caspase-9 Assay Kit (Colorimetric) | KTA3026 |
【Assay Procedure】
- Add samples, background control
and standard - Add Reaction Buffer and
substrate, incubate - Read samples at 405 nm
Simple
three steps, a big step in scientific research!
About Abbkine Scientific Co., Ltd.
Abbkine serves global scientists in the
field of proteomics and cytology and is committed to the innovation and
development of various scientific reagents related to proteomics and cytology,
expecting to accelerate the pace of life science research and drug discovery.
Proteomics products cover the preparation of samples (protein extraction,
purification, coupling), protein quantification, antibodies and kits for
protein detection. Cytology products involve cytokines (cell culture), cell
status detection, cell staining, organelle extraction, cell metabolism and
cytopathology reagents (kits). Abbkine relies on the product portfolio and
unique marketing support as the main market strategy and product innovation
mode, with ultimate aim to facilitate your research career.
Extensive research has indicated that intracellular organelles are integrated into cellular networks and collaborate on various cellular tasks rather than acting as isolated entities. Specialized membrane contact sites are formed between organelles, providing distinct spatial regions for executing and regulating their functions. Mitochondria is crucial dynamic organelles that participate extensively in many important cellular processes. Its dysfunction has been implicated in diverse diseases such as neurodegenerative disorders, cancer, and cardiovascular diseases.
