Abbkine Scientific has officially announced the release of its EliKine™ Human β-NGF ELISA Kit. The product also is known as the Human Beta-NGF ELISA Kit which is unique for its high sensitivity and excellent specificity.
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Nerve growth factor (NGF) is a neurotrophic factor and neuropeptide primarily involved in the regulation of growth, maintenance, proliferation, and survival of certain target neurons. β-NGF gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of β-NGF gene's expression is associated with allergic rhinitis.
The Human Beta-NGF ELISA Kit comes with different features and benefits that stands it out from its contemporaries on the market. This ELISA kit employs a two-site sandwich ELISA to quantitate β-NGF in samples, using colorimetric detection method. With a Sandwich ELISA (quantitative) assay type and working duration of between 3 and 5 hours depending on the operator, the kit looks to be a game changer in the industry.
This featured EliKine™ Human β-NGF ELISA Kit components that include Human β-NGF microplate, Human β-NGF standard, Human β-NGF detect antibody, EliKine™ Streptavidin-HRP, Standard diluent , Assay buffer , HRP substrate, Wash buffer, Stop solution and Plate covers, allows for easy use and manipulation, further endearing it to several investigators and researchers across the world. Our complete, ready-to-use ELISA Kits reduce assay time and variability and are available in either 1 or 10 pre-coated plate options.
About Scientific Co., Ltd.
Abbkine Scientific Co., Ltd. was founded in 2012. Our mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. We're devoting to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, and drug discovery. Abbkine is delighted to offer reproducible and sensitive EliKine ELISA kits for quantification of cytokines, hormones and other proteins with sandwich or competitive ELISA methods, individually.
More details please click www.abbkine.com.
2018年2月9日星期五
EliKine™ Human β-NGF ELISA Kit is Officially Released by Abbkine
2018年2月7日星期三
Abbkine Scientific announces the launch of Normal Rabbit Serum
With the fast development of scientific research, more and more life science research tools can be manufactured by global suppliers, in which I believe Abbkine scientific is the most active one. In this year, in order to better satisfy the various needs of scientific research workers, Abbkine scientific injected a large amount of finance of human resources to develop a new generation of blocking serum. Recently Abbkine Scientific announces the new release of Normal Rabbit Serum which is a proper member of Abbkine serum family.
The Normal Rabbit Serum is adopted from Rabbit and can be applied in BL, ELISA, ICC, IF, IHC experiment. The ideal start dilution ratio is 1:100 in ELISA, 1:10-20 in IF, ICC and IHC-p. it may depend on the different situation you meet in the experiment. But before using, you should store the Normal Rabbit Serum at 2-8°C or below -20°C for a certain period of time. The method you store it may also affect your experiment result.
Normal Rabbit serums are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate incubation step before addition of the primary antibody.
Abbkine's Normal Rabbit Serum is only use research and is not intended for use in human or clinical diagnosis.
Abbkine Scientific will continuously develop more products which are both high in quality and competitive in price. Our product will be greatly enlarged during our rapid development pace. The brand awareness can be a key factor to some extent, so we expect more and more scientific researchers to try our products, you won’t regret.
About Abbkine Scientific Co., Ltd.
Abbkine Scientific Co., Ltd. was founded in 2012. Our mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. We're devoting to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, and drug discovery. Abbkine is delighted to offer reproducible and sensitive EliKine ELISA kits for quantification of cytokines, hormones and other proteins with sandwich or competitive ELISA methods, individually.
2018年2月2日星期五
Abbkine Scientific newly introduces LinKine™ AbFluor™ 647 Labeling Kit to Kit family
The LinKine™ AbFluor™ 647 Labeling Kit is the newest product among LinKine™ conjugation kits. With the new release of this product, Abbkine makes a revolutionary step in the supply of life science research tools. We genuinely hope the LinKine™ AbFluor™ 647 Labeling Kit will provide a better choice for global scientific works in the current market.
Now Alexa Fluor dyes are frequently used as cell and tissue labels in fluorescence microscopy and cell biology. The individual members of the family are numbered according roughly to their excitation maxima (in nm). AbFluor™ 647 is a far-red fluorescent dye optimally excitable by the 633 nm argon laser line, which is a superior alternative to Cy5, Alexa Fluor 647.
During the experiment, you are bound to know that the original concentration of the molecules labelled should be more than 2mg/ml. Since it is convenient for researchers to maintain a single-use vials of reagent. The featured benefits of LinKine™ AbFluor™ 647 Labeling Kit also include highly-efficient purification, because the kit contains purification columns, which will remove the non-reacted dye and get a excellent protein recovery.
Except the purification columns, the LinKine™ AbFluor™ 647 Conjugation Kit also has Activated AbFluor 647 solution and AbFluor 647 labeling solution, all of these will do great help to carry on the experiment. The kit is definitely excellent in quality, if you are interest in it, you got the point.
About Abbkine Scientific Co., Ltd.
Abbkine Scientific Co., Ltd. was founded in 2012. Our mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. We're devoting to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, and drug discovery. Abbkine is delighted to offer reproducible and sensitive EliKine ELISA kits for quantification of cytokines, hormones and other proteins with sandwich or competitive ELISA methods, individually.
2018年1月26日星期五
EliKine™ Mouse IL-5 ELISA Kit is the new breakthrough from Abbkine Scientific
EliKine™ Mouse IL-5 ELISA Kit is the latest addition to Abbkine EliKine™ ELISA Kits family, after the scientific research giant announced the release of the product. The release of the kit is part of Abbkine’s attempt to ease the process of scientific research and investigation, making it more effective and allowing researchers to get more from their effort.
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The Mouse IL5 ELISA Kit as it also known a two-site sandwich ELISA to quantitate IL-5 in samples. The Interleukin 5 Mouse ELISA Kit uses the colorimetric for detection, with sample types including cell culture supernatants, other biological fluids, plasma, and serum.
One of the outstanding features of the kit in addition to high sensitivity and excellent specificity for detection of Mouse IL-5 is its assay duration. The kit has multiple steps standard sandwich ELISA assay, with a working time of 3 to 5 hours depending on the experience of the person carrying out the operation.
The EliKine™ IL-5 Mouse ELISA Kit includes Mouse IL-5 microplate, Mouse IL-5 standard, Mouse IL-5 detect antibody, EliKine™ Streptavidin-HRP, Standard diluent, Assay buffer, HRP substrate, Stop solution, Wash buffer as well as Plate covers.
About Abbkine Inc.
Abbkine Scientific Co., Ltd. was founded in 2012. Our mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. We're devoting to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, and drug discovery. Abbkine is delighted to offer reproducible and sensitive EliKine ELISA kits for quantification of cytokines, hormones and other proteins with sandwich or competitive ELISA methods, individually.
A new method to inhibit cancer cells—a mixture of engineered probiotics with vegetables
Colorectal cancer is one of the most common cancers in the world, and its incidence is predicted to rise in the next few years. Also, scientists have been working hard at finding a cure for this problem.
Researchers at the National University of Singapore have identified a way to turn a humble cocktail of bacteria and vegetables into a targeted system that seeks out and kills colorectal cancer cells. Led by Dr Chun-Loong Ho, this study had been published in Nature Biomedical Engineering.
The most important part of this cancer-targeting system is an engineered form of E. coli Nissle, a harmless type of bacteria found in the gut. They engineered the bacteria into a probiotic that attached to the surface of colorectal cancer cells and secreted an enzyme to convert a substance found in cruciferous vegetables (such as broccoli) into a potent anticancer agent.
Normal cells cannot do this conversion, and are not affected by the toxin, thus the system can be targeted only to colorectal cancer cells. The research aimed to kill cancer cells in the gut by allowing them to take up this anticancer agent.
Orally administered engineered microbes bind to the surfaces of colorectal cancer cells (a), allowing microbes to secrete myrosinase, which converts dietary glucosinolate found in cruciferous vegetables (b). When the cancer cells are cleared, the microbes are released from the surface of the intestinal wall (c).
Results find that, the mixture of engineered probiotics with a broccoli extract or water containing the dietary substance can killed more than 95% of colorectal cancer cells tested. Moreover, the mixture had no effect on cells from other types of cancer such as breast and stomach cancer.
Observably, in mice with colorectal cancer, the probiotics-veggie combination decreased tumour numbers by 75%. In addition, compared with control mice which were not fed with the mixture, the tumors found in these mice were three times smaller.
Scientists imagine that these probiotics might serve dual purposes, as prevention or clean up the cancer cells remaining after surgical removal of tumours. In the future, colorectal cancer patients may could take the probiotics as a dietary supplement along with their broccoli to prevent colorectal cancer or to reduce recurrence after cancer surgery.
"One exciting aspect of our strategy is that it just capitalizes on our lifestyle, potentially transforming our normal diet into a sustainable, low-cost therapeutic regimen. We hope that our strategy can be a useful complement to current cancer therapies." said Matthew Chang, associate professor at the National University of Singapore.
Read more, please click:
https://eurekalert.org/pub_releases/2018-01/nuos-wts011018.php
https://www.news-medical.net/news/20180111/Scientists-develop-genetically-engineered-probiotic-that-targets-and-kills-cancer-cells-in-the-gut.aspx
2018年1月24日星期三
LinKine™ AbFluor™ 488 Labeling Kit becomes a new member of Abbkine Kits family
With the acceleration of research and development, Abbkine Scientific spares no efforts to develop a new generation of LinKine™ Labeling Kits, The kits include AbFluor 350, 405,488, 555,594,647,680,770 and HRP, Biotin, FITC, Cy3 Labeling Kits. The LinKine™ AbFluor™ 488 Labeling Kit soon becomes one of the best-selling products due to its high quality and cost-performance. We all believe that the new launch of this product will be a revolutionary step for Abbkine Sci.
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AbFluor™ dyes are a series of highly water-soluble fluorescent dyes spanning full UV-visible and near-infrared spectrum for labeling biomolecules, especially proteins and nucleic acids. Their hydrophilic property makes the protein conjugation readily performed in aqueous media, minimizing the use of organic solvents. AbFluor™ dyes not only have much better labeling performance than other fluorescent labeling dyes such as FITC, TRITC, Cy® dyes, Alexa Fluor® and Dylight® labeling dyes, but also far more stable than the antibodies or other biomolecules they label.
The featured benefits of LinKine™ AbFluor™ 488 Labeling Kit include Single-use fluors and highly-efficient purification, which will guarantee the rapid and correct removal of non-reacted dye. Compared with different Kits on the current market, the LinKine™ AbFluor™ 488 Labeling Kit will absolutely be a better choice for researchers to conjugate AbFluor™ 488.
Abbkine is aimed at providing high quality research tools for global scientific workers. Our pursuit will never change, so Abbkine Sci will continuously develop now product at the scientist’s choice to accelerate the pace of biological research, which will also do great help to life science field.
About Abbkine Scientific Co., Ltd.
Abbkine Scientific Co., Ltd. was founded in 2012. Our mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. We're devoting to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, and drug discovery. Abbkine is delighted to offer reproducible and sensitive EliKine ELISA kits for quantification of cytokines, hormones and other proteins with sandwich or competitive ELISA methods, individually.
2018年1月18日星期四
RAS dimers play an important role in causing cancer
Ras is a family of related proteins which is expressed in all animal cell lineages and organs, which are involved in transmitting signals within cells. Mutations in ras genes can lead to the production of permanently activated Ras proteins, and this can cause unintended and overactive signaling inside the cell. Overactive Ras signaling can ultimately lead to cancer.
Mutated RAS genes are some of the most common genetic drivers of cancer, especially in aggressive cancers like pancreatic and lung cancer, so Ras inhibitors had been long studied for treating cancer. However, no medicines that target RAS are found despite decades of efforts.
Researchers at UT Southwestern's Simmons Cancer Center have shown that RAS molecules act in pairs, known as dimers, to cause cancer, findings that could help guide them to a treatment.
About the question of RAS dimerization, researchers have a heated discussion. Because no one can prove what RAS dimers look like, this limited the ability to design experiments that assess their importance in normal physiology and cancer. The UT Southwestern team led by Dr. Westover used X-ray crystallography data to predict what a RAS dimer might look like, then tested the model in cells using a method called fluorescence resonance energy transfer (FRET) to show when RAS forms dimers and when it does not.
The study provides a foundation for further studies that delve into RAS biology and could potentially pave the way to develop new cancer drugs that target RAS dimerization.
"The primary function of RAS is to transmit signals that tell a cell to grow and divide, a pathway commonly hijacked in cancer. What became clear in our studies is that RAS needs to dimerize to efficiently pass signals in cells. Moreover, RAS dimerization appears to be a crucial event for mutated forms of RAS to cause cancer," said Dr. Westover, part of the Simmons Lung Cancer Team.
Members of the Westover research lab teamed up with researchers from the Dana Farber Cancer Institute to show that RAS dimers are essential in a number of cancer cell systems and animal models of cancer.
Read more, please click http://www.utsouthwestern.edu/newsroom/articles/year-2018/ras-dimers.html