Abbkine Scientific announces the launch of Normal Goat Serum

With the fast development of scientific research, more and more life science research tools can be manufactured by global suppliers, in which I believe Abbkine scientific is the most active one. In this year, in order to better satisfy the various needs of scientific research workers, Abbkine scientific injected a large amount of finance of human resources to develop a new generation of blocking serum. Recently Abbkine Scientific announces the new release of Normal Goat Serum which is a proper member of Abbkine serum family.

The Normal Goat Serum is adopted from goat and can be applied in BL, ELISA, ICC, IF, IHC experiment. The ideal start dilution ratio is 1:100 in ELISA, 1:10-20 in IF, ICC and IHC-p. it may depend on the different situation you meet in the experiment. But before using, you should store the Normal Goat Serum at 2-8°C or below -20°C for a certain period of time. The method you store it may also affect your experiment result.

Normal goat serums are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate incubation step before addition of the primary antibody.

Abbkine Scientific will continuously develop more products which are both high in quality and competitive in price. Our product will be greatly enlarged during our rapid development pace. The brand awareness can be a key factor to some extent, so we expect more and more scientific researchers to try our products, you won’t regret.

About Abbkine Scientific Co., Ltd.

Abbkine Scientific Co., Ltd. was founded in 2012. Our mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. We're devoting to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, and drug discovery. Abbkine is delighted to offer reproducible and sensitive EliKine ELISA kits for quantification of cytokines, hormones and other proteins with sandwich or competitive ELISA methods, individually.

EliKine™ Rat IL-1β ELISA Kit joins Abbkine kits family

[caption id="attachment_82776" align="alignleft" width="308"] Rat IL-1β is detected by featured EliKine™ Rat IL-1β ELISA Kit[/caption]

With the rapid development of scientific research, Abbkine Scientific adds a new member to EliKine™ kits family. The new release of EliKine™ Rat IL-1β ELISA Kit will largely strengthen the core-competitiveness of Abbkine ELISA Kit group. Abbkine Scientific is aimed at making high quality life science research tools affordable, so the EliKine™ Rat IL-1β ELISA Kit is extremely competitive in price, which will give customer an easy entrance.

The high sensitivity and excellent specificity to detect Rat IL-1β will be a big advantage of this product, they are the main concern for scientist to choose research tools.

Interleukin 1 beta (IL1β) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a cytokine protein that in humans is encoded by the IL1B gene. There are two genes for interleukin-1 (IL-1): IL-1 alpha and IL-1 beta (this gene). IL-1β precursor is cleaved by cytosolic caspase 1 (interleukin 1 beta convertase) to form mature IL-1β. The molecular weight of the proteolytically processed IL-1β is 17.5 kDa. Increased production of IL-1β causes a number of different autoinflammatory syndromes, most notably the monogenic conditions referred to as CAPS, due to mutations in the inflammasome receptor NLRP3 which triggers processing of IL-1B.

EliKine™ Rat IL-1 beta ELISA Kit employs a two-site sandwich ELISA to quantitate IL-1β in samples. Three are no cross-reactivity or interference between Rat IL-1β and analogues. The kit is composed of Rat IL-1β microplate, Rat IL-1β standard, Rat IL-1β detect antibody, EliKine™ Streptavidin-HRP, Standard diluent, Assay buffer, HRP substrate, Stop solution, Wash buffer, Plate covers. You can use it with the most optimized procedures.

The EliKine™ series products are becoming more and more popular, Abbkine Scientific will also keep on developing more cost-effective products for end-users.

About Abbkine Scientific Co., Ltd.

Abbkine Scientific Co., Ltd. was founded in 2012. Our mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. We're devoting to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, and drug discovery. Abbkine is delighted to offer reproducible and sensitive EliKine ELISA kits for quantification of cytokines, hormones and other proteins with sandwich or competitive ELISA methods, individually.

A new research revealed that mutations in ADCY3 gene are related to obesity

Recently, an article entitled “Loss-of-function mutations in ADCY3 cause monogenic severe obesity” was published in Nature Genetics. This research highlighted ADCY3 as an important mediator of energy homeostasis and an attractive pharmacological target in the treatment of obesity. The research contents are as follows.

The scientists identify and functionally characterize homozygous mutations in the ADCY3 gene in children with severe obesity from consanguineous Pakistani families, as well as compound heterozygous mutations in a severely obese child of European-American descent.

When mutations occur in ADCY3, adenylate cyclase 3 it codes for forms abnormally and doesn't function properly. This leads to abnormalities relating to appetite control, diabetes, and even sense of smell.

“Early studies into ADCY3 tested mice that were bred to lack that gene, found that these animals were obese and also lacked the ability to smell, known as anosmia. When we tested our patients, we found that they also had anosmia, again showing a link to mutations in ADCY3.” said Professor Philippe Froguel, from the Department of Medicine at Imperial.



Based on the results, the researchers continue to study the possible mechanism. They entered their results into GeneMatcher, described by Professor Froguel as a “dating agency for genetics”. This led to another group of scientists in the Netherlands contacting the team with their own ADCY3 findings in one of their patients with obesity. This new European patient inherited different mutations on the same ADCY3 gene from both parents, so the ADCY3 gene of the offspring was not functioning properly, leading to obesity.

Further connections were made with a group of Danish scientists, studying the Inuit population of Greenland. Whilst not traditionally consanguineous (as in close family marriages), this population is small, so inbreeding is likely to have occurred.

Research into the genetic causes of obesity, and related conditions, could be incredibly valuable in finding ways to treat them. Currently, there are some drugs available or being tested, but knowing what specific mutations cause obesity allows scientists to create drugs that target them specifically.

‘’Obesity is not always gluttony, as is often suggested, and I think we should have a positive outlook considering the new treatments that are becoming possible.’’ said Professor Philippe Froguel, Chair in Genomic Medicine.

Read more, please click:

https://www.news-medical.net/news/20180108/Researchers-discover-mutations-in-a-gene-related-to-obesity.aspx

http://www3.imperial.ac.uk/newsandeventspggrp/imperialcollege/newssummary/news_8-1-2018-13-55-59

 

LinKine™ Labeling Kits

Total solutions for proteins and antibodies labeling with enzymes, fluorescence and featured AbFluor dyes.

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Abbkine announces the release of EliKine™ Human β-hCG ELISA Kit

[caption id="attachment_82713" align="alignleft" width="373"] Human β-hCG is detected by Abbkine's EliKine™ Human β-hCG ELISA Kit[/caption]

The EliKine™ Human β-hCG ELISA Kit, otherwise referred to as the Human β-hCG ELISA Kit is the new product from the stables of Abbkine Scientific, a life science solutions provider based in China. As part of the company’s strive to ensure a smoother and more effective research process in the field of life sciences, the company announced the release of the product.

Over the years, investigators and researchers have not particularly found it easy with the process of getting results in many of their endeavors. While advancements in science and technology have helped in creating innovative science tools, many of these tools are inaccessible to researchers, especially the relatively small ones. This is largely due to the cost of acquiring these tools.

However, Abbkine has continued to show support for investigators in the field of life science by release quality yet affordable EliKine™ ELISA Kits products, ensuring that everyone and anyone can easily access the products to get their desired results.

The EliKine™ Human Chorionic Gonadotropin beta ELISA Kit is another of such solutions. Employing a two-site sandwich ELISA to quantitate β-hCG in samples, and a colorimetric detection method, even with an assay duration that shows multiple steps standard sandwich ELISA assay with a working time of 2-3 hours, the kit is described as the desired life science research solution.

About Abbkine Scientific Co., Ltd.

Abbkine Scientific Co., Ltd. was founded in 2012. Our mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. We're devoting to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, and drug discovery. Abbkine is delighted to offer reproducible and sensitive EliKine ELISA kits for quantification of cytokines, hormones and other proteins with sandwich or competitive ELISA methods, individually.

Abbkine annouces the release of EliKine™ Mouse TGF-β1 ELISA Kit

[caption id="attachment_82708" align="alignleft" width="309"]              Standard Curve for Mouse TGF-β1[/caption]

The EliKine™ Mouse TGF-β1 ELISA Kit is the latest addition to the Abbkine Scientific family after the research company announced the official release of the product. Also referred to as the Mouse TGFB1 ELISA Kit, the product’s high sensitivity and excellent specificity for detection of Mouse TGF-β1 stand it out from its contemporaries on the market.

Abbkine is popular for its innovative solutions for researchers and investigators in the field of life science. Over the years, the company has come up with a wide range of antibodies and other such solutions designed to make researches and investigations easier and more effective, consequently creating better life science solutions.

The release of the Mouse LAP ELISA Kit follows the same direction of the other products from Abbkine, signaling a new dawn in the field of life science. Besides the products high sensitivity and excellent specificity for detecting Mouse TGF-β1, it employs a two-site sandwich ELISA to quantitate TGF-β1 in samples, with a working time of 3 to 5 hours depending on the experience of the person operating.

This unique features and benefits of the kit have ensured that it stays ahead of the competition and maintains Abbkine’s stand as one of the leading life science companies in the world.

About Abbkine Inc.

Abbkine Scientific Co., Ltd. was founded in 2012. Our mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. We're devoting to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, and drug discovery. Abbkine is delighted to offer reproducible and sensitive EliKine ELISA kits for quantification of cytokines, hormones and other proteins with sandwich or competitive ELISA methods, individually.

Lamin B1 Polyclonal Antibody Review

Lamin-B1 is a protein that in humans is encoded by the LMNB1 gene. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin

[caption id="attachment_1242" align="alignleft" width="154"] Lamin B1 Polyclonal Antibody in Western Blot analysis.[/caption]

structure, and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Lamin B, along with heterochromatin, is anchored to the inner surface of the nuclear membrane by the lamin B receptor.

Lamin B1 Polyclonal Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been tested with ELISA, IF, IHC-p, WB. And Abbkine suggested starting dilutions are as follows: WB: 1:500-1:2000, IHC-p: 1:100-1:300, ELISA: 1:40000.

LMNB1 encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of LMNB1 is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants. We make experiments with this Lamin B1 Polyclonal Antibody. The results of the experiment is satisfactory.