IFKine™ Orange Donkey Anti-Mouse IgG becomes the new addition to the Abbkine family

Abbkine Scientific Limited has recently announced the launch of its new product - IFKine™ Orange Donkey Anti-Mouse IgG. The Orange dye Donkey Anti-Mouse IgG conjugated antibodies are made to absorb light maximally at 555 nm and fluoresce with a peak around 570 nm. The antibody is brighter than its counterparties like TRITC, Cy3 antibody.

Hosted in donkeys and with mouse reactivity, the antibody’s immunogen is Mouse IgG whole molecule, with applications such as FCM, ICC, and IF. The antibody is polyclonal and is affinity purified using solid phase Mouse IgG (H&L) with finally > 95% purity based on SDS-PAGE.

Available in liquid solution, the antibody reacts with whole molecule mouse IgG. The antibody also reacts with light chains of all other mouse immunoglobulins. The minimization of the antibody’s cross-reaction with human, rabbit, goat, sheep and guinea pig serum proteins helps to reduce non-specific hybrization.

Abbkine IFKine™ is series of unique fluorence staining secondary antibodies with improved brightness, photostability and less nonspecific hybridization and background. The latest generation of IFKine fluorescent dyes ensure the best fluorescent performance, while it’s donkey host and other species of serum/IgG absorbed make IFKine™ secondary antibodies the ideal for use in fluorence staining, especially in fluorence multiple labeling.

About Abbkine Scientific Co. Ltd

Abbkine Scientific Co. Ltd is scientific research company founded in 2012 by number of scientists and marketing experts in the field of life science in California, USA. The company is headquartered in China and has effectively combined cutting edge technology from United States with China's manufacturing engineering and cost advantages to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research and drug discovery.

EliKine™ Human TSH ELISA Kit Review

Thyroid-stimulating hormone (also known as thyrotropin, thyrotropic hormone, TSH, or hTSH for human TSH) is a pituitary hormone that stimulates the thyroid gland to produce thyroxine (T4), and then triiodothyronine (T3) which stimulates the metabolism of almost every tissue in the body. T4 and T3 are indicated in controlling normal development of children, including brain development, and a variety of other functions in the body. People who have too much (hyperthyroidism) or too little (hypothyroidism) of these hormones can develop very serious medical problems. TSH is a glycoprotein hormone synthesized and secreted by thyrotrope cells in the anterior pituitary gland, which regulates the endocrine function of the thyroid. In 1916, Bennett M. Allen and Philip E. Smith found that the pituitary contained a thyrotropic substance.

EliKine™ Human TSH ELISA Kit adopts a two-site sandwich ELISA to quantitate TSH in samples. An antibody specific for TSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TSH present is bound by the immobilized antibody. After removing any unbound substances, a HRP conjugated antibody specific for TSH is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TSH bound in the initial step. The color development is stopped and the intensity of the color is measured.

As a scientific research worker, I have already known that Abbkine occupies a leading position in antibody research and development. When I heard that Abbkine will launch a series of brand-new EliKine™ ELISA kit, I ordered one without hesitation. To my surprise, EliKine™ Human TSH ELISA Kit has high sensitivity and excellent specificity for detection of Human TSH. No significant cross-reactivity or interference between Human TSH and analogues was observed. Everything goes smoothly within my project timelines. I believe that this product will also satisfy your concrete research needs perfectly. Abbkine enjoys a sound reputation for its advanced craftsmanship, high quality, attractive price and convenient delivery service. I hold a strong belief that Abbkine will be your primary choice in scientific research. If you trust me, you can order it right now!

ELISA Testing Service

Accelerate cytokines, chemokine, hormones and other proteins validation and detection with proprietary EliKine^TM ELISA Kits within 7 business days.

Why Choose Abbkine ELISA Testing Service for your Research?

•  High quality test tools- EliKine^TM ELISA Kits

EliKine™ series of ELISA kits exert high sensibility and specificity, with a comprehensive selection of ELISA kits available for the quantification of cytokines, chemokine, hormones and other proteins, to meet experiment demands. More features about EliKine^TM ELISA Kits, please visit https://www.abbkine.com/elikine-elisa-kits-new-addition-abbkines-scientific-research-kit-family/.

• Assays optimized for many sample types

We’ve tested our assays extensively in many different sample types. All analytes are validated for use with serum, plasma (EDTA, heparin, citrate) and cell culture supernatant. If you have a sample type we haven’t tried before, just let us know and we can help.

• Services from experienced and trustworthy scientist

Our dedicated ELISA Testing Service staff ensures your samples are treated in full accordance with the contracted study requirements. We provide rapid delivery of results and unsurpassed customer service. Our Testing Service laboratory successfully provides services for contract research organizations, academic groups, government organizations, and pharmaceutical companies.

• Quality assurance and timely delivery

Entrusting our expert personnel with your study samples ensures accurate results that are returned in a timely, efficient, and customized manner under our proven quality management systems. With our team of highly experienced scientists, Abbkine’s service department provides you with data of the highest accuracy and reproducibility.

To obtain an ELISA service quotation, contact us at service@abbkine.com

EliKine™ Human Prolactin ELISA Kit review

Prolactin (gene name PRL) is a secreted neuroendocrine pituitary hormone that acts primarily on the mammary gland to promote lactation, but has pleiotropic effects in both males and females. Prolactin is predominantly found as 199 amino acid, 25 kDa glycosylated and 23 kDa non-glycosylated monomers. Human prolactin shares only 60% and 63% amino acid sequence identity with mouse and rat prolactin, respectively, although rat prolactin can activate the human prolactin receptor. Although better characterized in rodents than humans, post-translational modifcations such as polymerization, glycosylation, and proteolytic cleavage can alter the activities of prolactin.

EliKine™ Human Prolactin ELISA Kit employs a two-site sandwich ELISA to quantitate Prolactin in samples. An antibody specific for prolactin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any prolactin present is bound by the immobilized antibody. After removing any unbound substances, a HRP conjugated antibody specific for prolactin is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of prolactin bound in the initial step. The color development is stopped and the intensity of the color is measured.

EliKine™ Human Prolactin ELISA Kit yielded a robust data set that met current requirements as well as provided a new tool for future use, everything goes well within my project timelines. This was due in large part to the excellent communication and impressive initiative taken by the scientists and product manager who used Abbkine extensive knowledge to find the best solution to my requirements.

EliKine™ Human IL-1β ELISA Kit – the new addition to Abbkine’s scientific research kit family

The EliKine™ Human IL-1β ELISA Kit is one of the latest scientific research products from Abbkine Scientific Co. Ltd. The company recently announced the launch of the product, designed to enhance scientific research processes.

The IL-1β is a member of the interleukin 1 cytokine family, produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). Interleukin 1β is an important mediator of the inflammatory response, involved in several cellular activities, including cell proliferation, differentiation, and apoptosis.

Interleukin 1β Elisa kit has human reactivity, employing a two-site sandwich ELISA to quantitate IL-1β in samples. The IL1B Elisa kit uses colorimetric method of detection, with the suitable samples types being Cell culture supernatants, other biological fluids, Plasma, and Serum.

The components of the kit include:

• Human IL-1β microplate


• Human IL-1β standard


• Human IL-1β detect antibody


• EliKine™ Streptavidin-HRP


• Standard diluent


• Assay buffer


• HRP substrate


• Stop solution


• Wash buffer


• Plate covers.

One of the major features and benefits of the kit is its high sensitivity and excellent specificity for detection of Human IL-1β., with no significant cross-reactivity or interference between Human IL-1β and analogues.

About Abbkine Scientific Co. Ltd

Abbkine Scientific Co. Ltd was founded in 2012 by a team of scientists and marketing experts in the life science field in California, USA. Headquartered in China, Abbkine has effectively combined cutting edge technology from United States with China's manufacturing engineering and cost advantages to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research and drug discovery.

EliKine™ Human bFGF ELISA Kit Review

The protein encoded by FGF2 gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.

EliKine™ Human bFGF ELISA Kit employs a two-site sandwich ELISA to quantitate bFGF in samples. An antibody specific for bFGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any bFGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for bFGF is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of bFGF bound in the initial step. The color development is stopped and the intensity of the color is measured.

I ordered the Human bFGF ELISA Kit from Abbkine and want to detect endogenous bFGF level in human cell culture supernatant. What is surprising is that I received the product just three days later. The technical support is very professional and patient, and they help me search the references and analyze the questions met during the experiment. Finally, I got satisfying results. The kit has high sensitivity and the price is cheaper than other brands. I want to recommend it to my friends.

EliKine™ Human FSH ELISA Kit review

Follicle Stimulating Hormone (FSH) is a glygoprotein produced by the anterior pituitary gland. In the female, FSH stimulates follicular growth, prepares ovarian follicles for action by LH and enhances the LH induced release of estrogen. FSH levels are elevated after menopause, castration and in premature ovarian failure. Although there are significant exceptions ovarian failure is indicated when random FSH concentrations exceed 40 mIU/ml. In the male, FSH stimulates seminiferous tubule and testicular growth and is involved in the early stages of spermatogenesis. Oligospermic males usually have elevated FSH levels. Tumors of the testes generally depress serum FSH concentrations, but levels of LH are elevated. High levels of FSH in men may be found in primary testicular failure and Klinefelter syndrome. Elevated concentrations are also present in cases of starvation, renal failure, hyperthyroidism, and cirrhosis.

EliKine™ Human FSH ELISA Kit employs a two-site sandwich ELISA to quantitate FSH in samples. An antibody specific for FSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FSH present is bound by the immobilized antibody. After removing any unbound substances, a HRP conjugated antibody specific for FSH is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FSH bound in the initial step. The color development is stopped and the intensity of the color is measured.

EliKine™ Human FSH ELISA Kit was designed to detect Human FSH in different kinds of samples, such as Plasma, Serum and other biological fluids. The detect range of this kit is 2 IU/L-70 IU/L and the minimum detection value is 1.0 IU/L. Our test proved the kit has high sensitivity and excellent specificity for detection of Human FSH. No significant cross-reactivity or interference between Human FSH and analogues was observed. The whole working time of our assay is about 3 hours. Both purchase and test process go well.