Abbkine Scientific

2017年6月9日星期五

Structure of the human multidrug transporter ABCG2

Topics overview: Treatment with iExosomes suppressed cancer in multiple mouse models of pancreatic cancer and significantly increased overall survival, How oncogenes and tumour suppressors promote nutrient uptake and thereby support the survival and growth of cancer cells, Scientist uncover a new inducer of mammalian heart regeneration, highlighting fundamental roles of the ECM in cardiac repair, Cryptosporidium lipid kinase PI(4)K (phosphatidylinositol-4-OH kinase) is a target for pyrazolopyridines and Structure of the human multidrug transporter ABCG2.

1. Exosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer

The mutant form of the GTPase KRAS is a key driver of pancreatic cancer but remains a challenging therapeutic target. Exosomes are extracellular vesicles generated by all cells, and are naturally present in the blood. Here Sushrut Kamerkar at University of Texas MD Anderson Cancer Center in Texas, USA and his colleagues show that enhanced retention of exosomes, compared to liposomes, in the circulation of mice is likely due to CD47-mediated protection of exosomes from phagocytosis by monocytes and macrophages. Exosomes derived from normal fibroblast-like mesenchymal cells were engineered to carry short interfering RNA or short hairpin RNA specific to oncogenic Kras^G12D, a common mutation in pancreatic cancer. Compared to liposomes, the engineered exosomes (known as iExosomes) target oncogenic KRAS with an enhanced efficacy that is dependent on CD47, and is facilitated by macropinocytosis. Treatment with iExosomes suppressed cancer in multiple mouse models of pancreatic cancer and significantly increased overall survival. Their results demonstrate an approach for direct and specific targeting of oncogenic KRAS in tumours using iExosomes.

2. Nutrient acquisition strategies of mammalian cells

Mammalian cells are surrounded by diverse nutrients, such as glucose, amino acids, various macromolecules and micronutrients, which they can import through transmembrane transporters and endolysosomal pathways. By using different nutrient sources, cells gain metabolic flexibility to survive periods of starvation. Quiescent cells take up sufficient nutrients to sustain homeostasis. However, proliferating cells depend on growth-factor-induced increases in nutrient uptake to support biomass formation. Here, Wilhelm Palm at Memorial Sloan Kettering Cancer Center in New York, USA and his colleagues review cellular nutrient acquisition strategies and their regulation by growth factors and cell-intrinsic nutrient sensors. They also discuss how oncogenes and tumour suppressors promote nutrient uptake and thereby support the survival and growth of cancer cells.

3. The extracellular matrix protein Agrin promotes heart regeneration in mice

The adult mammalian heart is non-regenerative due to the post-mitotic nature of cardiomyocytes. The neonatal mouse heart can regenerate, but only for the first week of life. Here Elad Bassat at Weizmann Institute of Science in Rehovot, Israel and his colleagues show that changes in the composition of the extracellular matrix (ECM) during this week can affect cardiomyocyte growth and differentiation in mice. They identify Agrin, a component of neonatal ECM, as required for the full regenerative capacity of neonatal mouse hearts. In vitro, recombinant Agrin promotes the division of mouse and human iPSC-derived cardiomyocytes via a mechanism that involves the disassembly of the dystrophin glycoprotein complex and Yap and ERK-mediated signaling. In vivo, a single administration of Agrin promotes cardiac regeneration in adult mice after myocardial infarction, although the degree of cardiomyocyte proliferation observed in this model suggests additional therapeutic mechanisms. Collectively, they uncover a new inducer of mammalian heart regeneration, highlighting fundamental roles of the ECM in cardiac repair.

4. A Cryptosporidium PI(4)K inhibitor is a drug candidate for cryptosporidiosis

Diarrhoeal disease is responsible for 8.6% of global child mortality. Recent epidemiological studies found the protozoan parasite Cryptosporidium to be a leading cause of paediatric diarrhoea, with particularly grave impact on infants and immunocompromised individuals. There is neither a vaccine nor an effective treatment. Here Ujjini H. Manjunatha at Novartis Institute for Tropical Diseases in Chromos, Singapore and his colleagues establish a drug discovery process built on scalable phenotypic assays and mouse models that take advantage of transgenic parasites. Screening a library of compounds with anti-parasitic activity, they identify pyrazolopyridines as inhibitors of Cryptosporidium parvum and Cryptosporidium hominis. Oral treatment with the pyrazolopyridine KDU731 results in a potent reduction in intestinal infection of immunocompromised mice. Treatment also leads to rapid resolution of diarrhoea and dehydration in neonatal calves, a clinical model of cryptosporidiosis that closely resembles human infection. Their results suggest that the Cryptosporidium lipid kinase PI(4)K (phosphatidylinositol-4-OH kinase) is a target for pyrazolopyridines and that KDU731 warrants further preclinical evaluation as a drug candidate for the treatment of cryptosporidiosis.

5. Structure of the human multidrug transporter ABCG2

ABCG2 is a constitutively expressed ATP-binding cassette (ABC) transporter that protects many tissues against xenobiotic molecules. Its activity affects the pharmacokinetics of commonly used drugs and limits the delivery of therapeutics into tumour cells, thus contributing to multidrug resistance. Here Nicholas M. I. Taylor at University of Basel in Basel, Switzerland and his colleagues present the structure of human ABCG2 determined by cryo-electron microscopy, providing the first high-resolution insight into a human multidrug transporter. They visualize ABCG2 in complex with two antigen-binding fragments of the human-specific, inhibitory antibody 5D3 that recognizes extracellular loops of the transporter. They observe two cholesterol molecules bound in the multidrug-binding pocket that is located in a central, hydrophobic, inward-facing translocation pathway between the transmembrane domains. Combined with functional in vitro analyses, their results suggest a multidrug recognition and transport mechanism of ABCG2, rationalize disease-causing single nucleotide polymorphisms and the allosteric inhibition by the 5D3 antibody, and provide the structural basis of cholesterol recognition by other G-subfamily ABC transporters.

2017年6月8日星期四

PurKine™ Protein SulfyBind Resin is the latest addition to the Abbkine resin family

Wuhan, China. 430074, 8^th June 2017. Announcing the launch of its PurKine™ Protein SulfyBind Resin, Abbkine Scientific Company Limited stated the features and benefits of the PurKine™ Protein SulfyBind Resin, especially when compared with its peers in the market.

Formulated in a liquid solution with 50% slurry in 20% ethanol, PurKine™ Protein SulfyBind Resin is a simple and efficient system of affinity chromatography. Allowing covalent immobilization of sulfhydryl-containing peptides, protein, and other ligands to the medium agarose support for use in affinity purification procedures, the portfolio ensures optimization of the process. This subsequently leads to maximum protein yield, stability, and solubility.

Consisting of 90μm beads of highly cross-linked 4% agarose coupled with Iodoacetic acid, the performance of the resin has been reported to equal or even surpass popular SulfoLink resins from other suppliers.

Other features and benefits of the kit include Reduced Cysteine Residues and effective Antibody Purification. Its high capacity, cost-effectiveness, flexibility, and high performance are features that further distinguish it from its peers.

The PurKine™ Protein SulfyBind Resin is available in two basic formats - prepacked spin column and kit and has been tested to retain its effectiveness even after five repeated uses. This establishes the cost-effectiveness of the product.

About Abbkine Scientific Co. Ltd.

Abbkine Scientific Co. Ltd is a scientific research institute headquartered in China. Founded by a team of scientists and marketing experts, the serves the field of sciences by perfectly combining cutting edge technology from the United States with China's manufacturing engineering and cost advantages, to provide state-of-the-art recombinant proteins, antibodies, and other scientific research tools.

2017年6月7日星期三

HP-1γ Mouse Monoclonal Antibody (2F5) review

The family of Heterochromatin Protein 1 (HP1) (“Chromobox Homolog”, CBX) consists of highly conserved proteins, which have important functions in the cell nucleus. All three HP1 family members (α, β, and γ) are primarily associated with centromeric heterochromatin; however, HP1β and γ also localize to euchromatic sites in the genome. Members of the HP1 family are characterized by an N-terminal chromodomain and a C-terminal chromoshadow domain, separated by a Hinge region. HP1 is also found at euchromatic sites, where its binding correlates with gene repression. The protein encoded by CBX3（chromobox 3) binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5′ UTR, have been found for CBX3.

Abbkine HP-1γ Mouse Monoclonal Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody has been verified in IF, IHC-p, WB experiments and shows good effects. You can detect endogenous HP-1γ protein in the samples of Human, Mouse and Rat. About the dilutions, you need to design pre-experiment to determine the optimal concentration, which suits for your specimen.

As researchers, we all know that choosing an appropriate antibody is very important step for the success of an experiment. And I believe Abbkine antibody is a good choice for you. Two months ago, I purchased a HP-1γ Mouse Monoclonal Antibody from Abbkine to finish my graduation thesis. It’s very convenient to order items and I received the product very soon. Compared with other famous companies, the price is quite cost-effective with the same quality. During the process of my experiments, I met some problems. The technical staff offered me very professional guidance and help me analysis the reasons with great patience. Finally, I obtained satisfied results and graduated smoothly. Through the story of my experience, I strongly recommend AbbKine products for you.

Getting more research benefts from innovative Cell Counting Kit-8 with less cost!

Abbkine Cell counting kit-8 is a colorimetric assay kit for cell number counting, cell prolifration and cytotoxicity assays. By adding CCK-8 directly to the cell media, CCK-8 kit shows fast, high-throughput screening without a solubilization process, obtaining highly reproducible and accurate results.

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SuperLumia HRP ECL & ECL Plus Substrate Kits

Abbkine SuperLumia ECL HRP Substrate Kits are value-priced, classical peroxidase substrates for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

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2017年6月5日星期一

Review of CD5 Monoclonal Antibody

Abbkine Scientific is one of the most trusted names in the field of life science research, providing innovative research tools and products since it was founded some years back. The Chinese based scientific research company recently announced the official launch of its new product – the CD5 Monoclonal antibody.

The CD5 antibody as it is also referred to as is another product from the stables of the research giant. The product like many other tools from Abbkine Scientific is designed to help simplify and enhance research processes, leading to more effective results.

The T-cell surface glycoprotein CD5 antibody has come under scrutiny from different quarters, especially researchers and investigators alike. Below is a review of the product, detailing the features, benefits, pros and cons of the newly-launched antibody.

Overview of CD Monoclonal Antibody

CD5 also designated as Lyt-1 is a popular substance famous for being a trans-membrane glycoprotein, which is expressed on 70% of normal peripheral blood lymphocytes. It has also been identified on practically all T lymphocytes in thymus and peripheral blood.

In addition, the activation of T cells via the T cell receptor also known as TCR leads to tyrosine phosphorylation of CD5 and the absence of CD5 renders T cells hyper-responsive to TCR-mediated activation.

CD5 has been discovered to associate with TCR/CD3-z chain, and the Src family kinase Lck p56. Vitro studies have revealed a significant increase in the kinase activity of Lck bound to CD5, by as much as ten to fifteen folds.

CD72, which is the B cell antigen, serves as a receptor for CD5. The effect of CDG binding with its cognate receptor is not readily known at the moment. However, report has it that it plays a role in the selection of thymic.

Features of the CD Monoclonal Antibody

The CD5 Monoclonal antibody comes with a number of features or properties. While it shares some of the features with its counterparts, it possesses some unique attributes that distinguishes it from other such products.

Some of the features are mentioned below

Formulation – liquid solution

Concentration – 1 mg/ml

Immunogen – synthetic peptide

Host – Mouse

Reactivity – Human, Mouse, Rat

Applications – IF, IHC-p

Isotype – Mouse IgG1

Clonality – Monoclonal

Storage buffer – PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol

One of the unique features of the CD5 monoclonal antibody is the method of purification employed while producing it, as it is affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Another relatively unique feature of the antibody is the ability of investigators to determine the best working dilutions that suits the purpose of the experiment. While the manufacturer suggests starting dilutions of IHC-p: 1:200, IF: 1:200, investigators are advised to determine the optimal working dilutions experimentally.

The Pros of CD5 Monoclonal Antibody

Also known as LEU1 antibody, the product has been identified to have a number of pros especially when compared with other antibodies in its category. One of the pros of the product is its durability as users can store it for as long as one year at -20°C from date of shipment.

Another advantage of the product is its ability to detect endogenous CD5 proteins.

Its availability in a liquid solution and flexible dilutions also allows for flexibility for investigators and researchers alike.

The Cons of CD5 Monoclonal Antibody

Currently, there are no known negative effects or cons of the CD5 Monoclonal Antibody. However, the manufacturers have warned against using it for human or clinical diagnosis, as it is strictly for research purposes only.

Conclusion

The CD5 Monoclonal Antibody is another great product from Abbkine Scientific Company Limited and tends to satisfy the desire of most scientific investigators and researchers.

2017年6月2日星期五

Abbkine Scientific launches the PurKine™ Protein AminoBind Resin 4FF for researchers and investigators

Wuhan, China. 430074, 2^nd June 2017. The PurKine™ Protein AminoBind Resin 4FF is a new product from the stables of the famous international scientific research company, Abbkine Scientific. The product is designed to aid researchers and investigators alike, by ensuring optimum performance.

PurKine™ Protein AminoBind Resin 4FF is crosslinked having 4% beaded agarose resin, which contains N-hydroxysuccinimide (NHS) functional groups. Otherwise called the NHS-Activated Agarose Resin, the product can be used for the preparation of affinity purification procedures, which can isolate specific substances from complex mixtures, helping to achieve very high purity in a single step.

The resin is a pre-activated agarose matrix that increases the choice of coupling chemistries available. It is suitable for amino-containing smaller proteins and peptides coupling. It is also great for scaling-up due to its high flow properties.

The resin is unlike any other NHS-Activated Sepharose, with distinguishing features and benefits. Some of them include flexibility, high capacity, and cost-effectiveness. Formulated in liquid solution with 50% slurry in 100% isopropanol, the product can be easily used by researchers and investigators.

Abbkine Scientific has clearly stated the prohibition of the use of the product for clinical or human diagnosis as it is exclusively manufactured for research purpose.

About Abbkine Scientific Co. Ltd.

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The company has subsequently established itself as a scientific research heavyweight with the provision of generic and customized solutions to clients across the globe.

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