PurKine™ Anti-DDDDK Tag Resin 4FF is the latest addition to the Abbkine PurKine™ resin family

Wuhan, China. 430074, 11^th May 2017. Abbkine Scientific has announced the launch of its new product, the PurKine™ Anti-DDDDK Tag Resin 4FF, otherwise known as Flag resin. The resin has gained popularity for being used in the affinity purification of Flag fusion proteins.

Consisting of 90μm beads of cross-linked 4% agarose, coupled with mouse monoclonal antibody against DYKDDDDK tag, the DDDDK Resin allows for optimization of the process. This subsequently leads to maximum protein yield, solubility, and stability.

The durability of the product, with several tests confirming no decrease in, is performance after more than five repeated uses is another premium feature of the product. Containing high flow properties, the PurKine™ Anti-DDDDK Tag Resin 4FF is a perfect choice for scaling-up.

Other features and benefits of the resin include high capacity with over 1mg DYKDDDDK-tagged protein per mL of resin and cost-effectiveness due to the possibility of several usages. It also has flexibility from being available in multiple formats that include bulk resin, spin columns and complete kits, and its “robustness” as its highly crosslinked beads tolerate linear flow rates up to 300cm/hour.

As a Liquid solution with 50% slurry in TBS containing 0.02% sodium oxide, the resin is available in prepacked spin column and kit formats.

Made exclusively for research use only, the manufacturer strongly prohibits the use of the product in human or clinical diagnosis.

About Abbkine Scientific Co. Ltd.

Abbkine Scientific Co. Ltd is a scientific research institute headquartered in China. Founded by a team of scientists and marketing experts, the serves the field of sciences by perfectly combining cutting edge technology from the United States with China's manufacturing engineering and cost advantages, to provide state-of-the-art recombinant proteins, antibodies, and other scientific research tools.

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The company has subsequently established itself as a scientific research heavyweight with the provision of generic and customized solutions to clients across the globe.

NSE Monoclonal Antibody Review

NSE, or Enolase 2 (gamma, neuronal), is a human gene encoded by ENO2. It may also be known as: Gamma-enolase; Enolase 2; Neural enolase; Neuron-specific enolase; Gamma-enolase ; HEL-S-279. The encoded protein is an enzyme with an amino acid length of 434 and a mass of 47.3 kDa. Other isoforms may exist. NSE is a member of the Enolase family. Homologs of this gene exist in other organisms, including: Mouse, Rat.

Abbkine NSE Monoclonal Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody detects endogenous NSE proteins. It’s suitable to be applied in WB, IHC and IF. It’s verified to react with human, mouse and rat. The concentration of this monoclonal antibody is 1mg/ml.

Our group purchased Abbkine NSE monoclonal antibody for detecting NSE proteins in paraffin-embedded Human-colon tissue sections. Our sample were incubated with Abbkine NSE monoclonal antibody (diluted at 1:200) overnight at +4°C. The sensitivity of the antibody was perfect. Thanks to our research partner-Bob recommending Abbkine brand to us, we plan to use them again.

CD21 Monoclonal Antibody – Review

Abbkine Scientific Company Limited is one of the very popular scientific solutions providers, with numerous tools, antibodies, resins and other such products coming from them. Therefore, it did not come as a surprise to many when it recently announced the release of its new monoclonal antibody – CD21 Monoclonal Antibody, otherwise known as the CD21 antibody.

Like most of the other antibodies and resins, the Complement receptor type 2 antibody has come different reviews from experts and other such stakeholders in the industry.

What is CD21 Monoclonal Antibody?

CD21 Monoclonal Antibody also known as the C3DR antibody, the antibody is a somewhat unique antibody with amazing features that has endeared it to many researchers and investigators across the globe.

CR2 encodes a membrane protein known as complement C3d receptor 2. The membrane protein is reputed to function as a receptor for Epstein-Barr virus (EBV) binding on B and T lymphocytes. In addition to this feature, the genetic variations in CR2 are susceptible to systemic lupus erythematosus type 9 also known as SLEB9. Alternatively, spliced transcript variants encoding different isoforms have been found for CR2.

Features of CD21 Monoclonal Antibody

The antibody comes with different features and benefits, some of which are briefly highlighted below

• Immunogen – Synthetic Peptide


• Host – Mouse


• Reactivity – mouse, human, and rat


• Applications – IF and IHC-p


• Clonality – monoclonal


• Isotype – Mouse IgG1


• Formulation – Liquid solution


• Concentration – 1 mg/ml


• Gene ID – 1380


• Storage buffer – PBS  pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol

CD21 Monoclonal Antibody – Pros

The features of the monoclonal antibody mentioned above have clearly showed some of the perceived benefits of pros of the product, especially when compared to others in the market.

One of such pros is the formulation of the antibody. As a liquid formulation, the antibody can be easily applied by researchers and investigators.

The purification process of the antibody also distinguishes it from its peers. The antibody was affinity-purified from mouse ascites by affinity chromatography using specific immunogen.

The storage buffer of PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol is also one of the pros of the product.

The antibody also detects endogenous CD21 proteins, another of its relatively many desirable features.

The CD21 Monoclonal antibody by Abbkine Scientific can be stored for as long as one year at -20°C from the date of shipment. The ease of storage and durability ensure that users can hold onto it for a long period.

There is also flexibility in its use as investigators can determine the optimal working dilutions by experiments, even as the suggested starting dilutions are IHC-p: 1:200, IF: 1:200.

CD21 Monoclonal Antibody – Cons

So far, there are no cons readily identified for the antibody. However, continuous of the use of the product by researchers and investigators alike would reveal possible drawbacks.

CD21 Monoclonal Antibody – Final Verdict

After a comprehensive assessment of the CD21 Monoclonal Antibody, one can confidently say that researchers and investigators have a reliable antibody to work with.

Ki 67 Monoclonal Antibody Review

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. It is strictly associated with cell proliferation. During interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. Ki-67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0).

Abbkine Ki 67 Monoclonal Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. It recognizes endogenous levels of murine Ki-67 protein. It will also detect endogenous levels of human Ki-67 protein. The antibody has been shown to work in IHC-p and IF. Abbkine suggested the starting dilutions are as follows: IHC-p: 1:200, IF: 1:200.

Ki-67 is an excellent marker to determine the growth fraction of a given cell population. The fraction of Ki-67-positive tumor cells (the Ki-67 labeling index) is often correlated with the clinical course of cancer. We studied mouse testis tissue for Immunofluorescence analysis using Abbkine Ki 67 Monoclonal Antibody. Fortunately, the performance of the antibody was excellent, an immunopositive signal was found.

Kif 7 Monoclonal Antibody Review

The KIF7 (kinesin family member 7) gene provides instructions for making a protein that is associated with structures called primary cilia. These microscopic, finger-like projections stick out from the surface of cells and are involved in signaling pathways that transmit information into cells. Studies suggest that the KIF7 protein helps to maintain the proper length and stability of cilia.

Abbkine Kif 7 Monoclonal Antibody  is a well characterized mouse monoclonal recommended for detecting KIF7 of human, mouse and rat origin by IF and IHC-p.  Supplied as three different sizes as liquid solution , the concentration of the purified antibody is 1mg/ml. The antibody is stable for one year at -20°C from date of shipment. Abbkine suggested aliquot to avoid repeated freezing and thawing.

Immunofluorescence analysis of Mouse-colon tissue using Abbkine Kif 7 Monoclonal Antibody (red), diluted at 1:200 (4°C,overnight). Dylight 549 labled anti-mouse was used as secondary antibody. The low background fluorescence yielded a high signal-to-noise ratio. I give them a high recommendation and plan to use them again.

Aquaporin 4 Monoclonal Antibody Review

Aquaporin-4, also known as AQP4, is a water channel protein encoded by the AQP4 gene in humans. AQP4 belongs to the aquaporin family of integral membrane proteins that conduct water through the cell membrane. Only limited aquaporins are found within the CNS: AQP1, 3, 4, 5, 8, 9, and 11, but only AQP1, 4, and 9 are exclusively found in the brain and spinal cord. Focusing on AQP4, which has the most water channels in the CNS, are specifically located at the perimicrovessel astrocyte foot processes, glia limitans, and ependyma. Aquaporin-4 was first discovered in 1986, it was the first evidence for the existence of water transport channels.

Abbkine Aquaporin 4 Monoclonal Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The isotype is Mouse IgG1. The antibody is suitable for WB, IF and staining Aquaporin 4 in IHC using paraffin embedded sections. It can react with Human, Mouse and Rat. Abbkine suggested optimal working dilutions should be determined experimentally by the investigator.

Immunohistochemical analysis of Human liver cancer tissue, labelling Aquaporin 4 with Abbkine Aquaporin 4 Monoclonal Antibody , diluted 1/200. Shows strong membranous staining of tubular epithelial cells. Finally, the customer service was superb. This was especially appreciated given that I am in Japan and they are in China.

Abbkine announces the release of its new antibody - IPKine™ HRP Goat Anti-Mouse IgG HCS

Wuhan, China. 430074. 26^th April 2017. Abbkine Scientific is no doubt one of the world’s leading scientific research companies and the institution recently announced the addition of another antibody into the IPKine family. The IPKine™ HRP Goat Anti-Mouse IgG HCS is an HRP Secondary Antibody designed to help researchers and scientists get the best out of their works without necessarily breaking the bank.

The Anti-Mouse Heavy Chain comes in a liquid solution with such features as heavy chain/Fc fragment specific optimization, helping to eliminate light chain interference. Its absorption of serum proteins also helps to minimize cross-reactivity with immunoglobulins from other species, which are some of its unique features.

The antibody reacts with Fc portion of mouse IgG heavy chain, while it doesn’t react with the Fab portion of mouse IgG. It's cross-reaction with human, bovine and horse serum proteins has been specialized minimized, while it may cross-react with immunoglobulins from other species. It has no reactivity on non-immunoglobulin serum proteins.

With its host being the goat, the antibody’s applications include WB, IP and others, hence a co-IP. For optimal working conditions, the dilutions should be determined by the investigator through experiment. However, the suggested solutions are WB 1:10,000-1:100,000, IHC 1:500-1:5,000 and ELISA 1:5,000-1:100,000.

The IPKine™ HRP Goat Anti-Mouse IgG HCS can be stored for as long as one year from the shipment date at a stable -20°C. However, the product is specifically designed for research use only and should not be used for clinical diagnosis or human use.

About Abbkine Scientific

Abbkine Scientific Company Limited is a life science research company headquartered in California. Founded in 2012, the establishment has been able to spread its tentacles across the globe with increasing presence and acceptance from Asia Pacific thanks to its continuous efforts to make the world a better place.

Abbkine combines cutting edge technology with manufacturing engineering and cost advantage to provide innovative, high-quality assay kits and other research and scientific products enhance life science fundamental research and drug discovery amongst others.