Abbkine Scientific’s new antibody aids easier and more effective IP and co-IP research

Wuhan, China. 430074. 13^th April 2017. IPKine™ HRP, Goat Anti-Mouse IgG LCS is the latest HRP Secondary Antibody from the scientific research giant, Abbkine Scientific. The product is released as part of the Abbkine's IPKine™ series designed to aid the process of research and enhance accuracy in results.

The Anti-Mouse Light Chain immunogen antibody is available in liquid solution, with mouse reactivity. The secondary antibody is made to be perfect for different applications which include Western Blot, IP and co-IP.

The antibody is made under strict quality control process, using a unique purification method. IPKine™ HRP, Goat Anti-Mouse IgG LCS, is affinity purified with over 95% based on SDS-PAGE using solid phase Mouse IgG light chain.

Sourced from goat, the antibody does not react with the heavy chain of mouse IgG, while reacting with kappa light chains on mouse IgG. Unlike other antibodies, the IPKine™ HRP, Goat Anti-Mouse IgG LCS does not suffer from heavy or light chain blotting contamination, thanks to less background noise and enhanced accuracy. Its light fragment specific optimization also helps to eliminate heavy chain interference as the case may be.

IPKine™ HRP, Goat Anti-Mouse IgG LCS is specifically made for research purpose only and should not be administered on human or used for clinical diagnosis.

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About Abbkine Scientific

Abbkine Scientific Company Limited is a life science research company headquartered in California. Founded in 2012, the establishment has been able to spread its tentacles across the globe with increasing presence and acceptance from Asia Pacific thanks to its continuous efforts to make the world a better place.

Abbkine combines cutting edge technology with manufacturing engineering and cost advantage to provide innovative, high-quality assay kits and other research and scientific products enhance life science fundamental research and drug discovery amongst others.

CK16 Monoclonal Antibody Review

Keratin 16 is a protein that in human is encoded by the KRT16 gene. Keratin 16 is a type I cytokeratin. It is paired with keratin 6 in a number of epithelial tissues, including nail bed, esophagus, tongue, and hair follicles. Mutations in the gene encoding this protein are associated with the genetic skin disorders pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus.

Abbkine CK16 Monoclonal Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody detects endogenous levels of total Cytokeratin 16 protein. It reacts with  Human, Mouse and Rat. Test confirmed this antibody was suitable for IF and IHC-p. Abbkine suggested starting dilutions are as follows: IF: 1:200, IHC-p: 1:200. The isotype is Mouse IgG1.

Abbkine CK16 Monoclonal Antibody stained Rat liver tissue sections. The tissue sections were then incubated with the antibody (ABM40056, diluted at 1:200) overnight at 4°C. IFKine™ Orange Donkey Anti-Mouse IgG was used as secondary antibody at a 1/1000 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (blue). The signal produced was very strong and clear. The sensitivity of the antibody was also perfect.

CK8 Monoclonal Antibody Review

Keratin, type II cytoskeletal 8 also known as cytokeratin-8 (CK-8) or keratin-8 (K8) is a keratin protein that is encoded in humans by the KRT8 gene. It is often paired with keratin 18. Keratin 8 is often used together with keratin 18 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood. Antibodies to CK8 can be used to differentiate lobular carcinoma of the breast from ductal carcinoma of the breast.In normal tissue, it reacts mainly with secretory epithelia, but not with squamous epithelium, such as that found in the skin, cervix, and esophagus.

Abbkine CK8 Monoclonal Antibody is a high quality mouse monoclonal recommended for detecting endogenous Cytokeratin 8 of mouse, rat and human origin by WB, IF and IHC-P. The isotype is mouse IgG1. Thesuggested starting dilutions by abbkine are: WB: 1:2000-5000, IF: 1:100-200 and IHC-p: 1:200. Optimal working dilutions should be determined experimentally by the investigator.

Abbkine CK8 Monoclonal Antibody staining Cytokeratin 8 in Human colon tissue by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formalin and subjected to heat-mediated antigen retrieval in citrate buffer (0.1M Sodium Citrate) prior to blocking with 5% serum for 1 hour at 4°C. The primary antibody was diluted 1/200 in 5% goat serum in PBS and incubated with the sample for 12 hours at 4°C. Goat anti-mouse was used as the secondary antibody. I have to say I was lucky. This product met my demands. It deserves to recommend.

Abbkine Scientific launches new product - IFKine™ Red Donkey Anti-Rabbit IgG

Wuhan, China. 430074. 8^th April 2017. The newly launched IFKine™ Red Donkey Anti-Rabbit IgG, is the latest discovery by renowned science research company, Abbkine Scientific. This antibody like the others from Abbkine, are designed to be used in several antibody-based applications.

IFKine™ Red conjugated antibodies absorb light maximally around 591 nm and fluoresce with a peak around 615 nm. They are brighter, more photostable, and more hydrophilic than other red-fluorescing conjugates.  IFKine™ Red antibodies are some of the secondary antibodies of high quality sourced from animals like goat and donkey, for professional scientific research purposes.

The IFKine™ Red Donkey Anti-Rabbit IgG is just one out of the series of Abbkine IFKine™ unique fluorence staining secondary antibodies that comes with features like photo stability, less nonspecific hybridization, and improved brightness.

The unique features of the product ensures that scientists get the best fluorescent performance, with its host – donkey, making it ideal for fluoresce staining, especially in fluoresce multiple labeling.

The Donkey is the host of the newly launched IFKine™ Red Donkey Anti-Rabbit IgG, with rabbit being the reactivity. The antibody is to be applied in FCM, IF, and ICC, with Rabbit IgG whole molecule immunogen.

The anti-rabbit IgG comes in a liquid solution and is Affinity purified using solid phase Rabbit IgG (H&L) with finally > 95% purity based on SDS-PAGE. The product is specifically designed for research purposes, with optimal dilutions determined by the investigator after series of experiments. However, the suggested dilution for most fluorescent applications starts from 1:50 to 1:1,000.

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About Abbkine Scientific

Abbkine Scientific Co. Ltd is a scientific research company founded in 2012 in the United States. Established by a team of scientists and marketing experts, the company moved its headquarters to China to meet up with the growing demand from the Asia Pacific.

The company has been able to combine cutting edge technology from United States and manufacturing engineering and cost advantages from China to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, amongst others.

CD68 Monoclonal Antibody Review

The CD68 Monoclonal Antibody is an antibody belonging to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. It is one of the newest products and discoveries from the stable of Abbkine Scientific, a leading scientific research company.

Otherwise referred to as CD68 Antibody, the CD68 also belongs to the scavenger receptor family. The features of the antibody and its unique functionalities have made it the preferred amongst scientific researchers.

CD68 Monoclonal Antibody – Unique features of the antibody

Unlike its peers, the CD68 antibody allows for easier and more effective research due to its unique attributes. Some of the attributes of the Gp110 antibody are briefly highlighted below.

• It allows for easy detection of endogenous CD68 proteins, ensuring more effective and goal-oriented scientific research works


• The CD68 belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family


• The antibody is also a member of the scavenger receptor family


• The product comes in a liquid solution


• It is a Mouse IgG1 isotype


• Immunogen – synthetic peptide


• Host – mouse


• Reactivity – rat, mouse and human


• Multiple applications – IF, IHC-p


• Clonality – monoclonal


• Concentration – 1 mg/ml


• Storage buffer – PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol


• Purification – The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.


• Storage instructions – Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Users should aliquot to avoid repeated freezing and thawing.

CD68 Monoclonal Antibody – Pros

Also referred to as Macrosialin, the antibody has been attributed to have its cons especially compared with other antibodies. Below are some of the features that stand the CD68 Monoclonal Antibody from the competition.

• It is highly expressed by human monocytes and tissue macrophages


• The antibody primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface


• It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins


• It belongs to the scavenger receptors family making it excellent for clearing cellular debris


• It is also great for promoting phagocytosis, and mediating the recruitment and activation of macrophages


• It provides alternative splicing results in multiple transcripts encoding different isoforms


• It can be easily stored in gel pack with blue ice


• Flexibility in determining the optimal working dilutions

CD68 Monoclonal Antibody – Cons

So far, the CD68 Monoclonal Antibody has not been attributed with a particular drawback or con as long as users follow the instructions given by the maker – Abbkine Scientific.

CD68 Monoclonal Antibody – Summary

The CD68 Monoclonal Antibody is one of the most effective antibodies in the market in terms of performance and cost-effectiveness. Reviews have continued to pour in from users across the globe. The antibody is however designed for research use only. The company takes no liability for violations in the use of the products and infringements that may occur with the use of the product.

Abbkine Scientific announces the launch of Cell Counting Kit-8 (CCK-8)

Wuhan, China. 430074. 4^th April 2017. Abbkine Scientific recently announced the launch of its cell proliferation and cell toxicity kit called the Cell Counting Kit-8, otherwise known as CCK-8. The kit is made to allow for the easy detection of cell toxicity and proliferation based on WST-8. The WST-8 approach adopted for the manufacturing of the kit gives an orange colored product due to the reduction by dehydrogenases in cells.

The formulation is in a liquid solution, with the kit containing CCK-8 solution. The CCK-8 uses highly water-soluble tetrazolium salt WST-8:[2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt] that changes to a water-soluble orange formazan dye by reduction.

The low toxicity of the Cell Counting Kit and the determination of the number of viable cells in cytotoxicity assays and cell proliferation due to its sensitive colorimetric assays are distinguishing features of the CCK-8.

The kit is a one-bottle ready to use solution, helping to reduce the time and stress involved in the process. Due to the water solubility of the product, no organic solvents or isotopes is required. The stability and safety of the formazan is another major benefit of the CCK-8 kit.

One of the most distinguishing factors of the kit when compared to other cell counting kit is its detection sensitivity feature that is higher than assays when other tetrazolium salts like XTT and MTT are used. The effectiveness of the kit surpasses that of its peers, with the ability to use the same cells for other cell assays thanks to the low toxicity of the Cell Counting Kit by Abbkine Scientific.

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About Abbkine Scientific

Abbkine Scientific Co. Ltd is a scientific research company founded in 2012 in the United States. Established by a team of scientists and marketing experts, the company moved its headquarters to China to meet up with the growing demand from the Asia Pacific.

The company has been able to combine cutting edge technology from United States and manufacturing engineering and cost advantages from China to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, amongst others.

CK7 Monoclonal Antibody Review

Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments. Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers . Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases. Keratin 7 and keratin 19 are present in hepatic and pancreatic progenitor/stem cells.

Abbkine CK7 Monoclonal Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. Supplied in PBS (pH 7.4) containing 0.02% sodium azide as preservative and 50% Glycerol, the antibody detects endogenous levels of total keratin 7 protein of  Human, Mouse and Rat samples. Tested applications for this antibody are IF, IHC-p, IP and WB.

Immunofluorescence analysis of human-liver tissue. Abbkine CK7 Monoclonal Antibody was diluted at 1:200 (4°C,overnight). Dylight 549 labled goat anti-mouse was used as secondary antibody. The test result showed a positive fluorescent reaction using the antibody, which provides a basis for our later research. Apart from the antibody’s performance, their customer service was superb. This was especially appreciated.