2020年2月28日星期五

The Statement of Strengthening Epidemic Prevention for COVID-19

Dear Colleagues,





The situation for COVID-19’s outbreak in Wuhan is getting improved in the strong supports of our government and caring people all over the world now. We are very lucky that we all are safe at present, while it’s still very important for us to do well protections. We determine to do below provisional measures during the resumption of production until the end of the outbreak in order to ensure the safe of all colleagues and our clients strictly.





  1. The establishment of epidemic prevention group, their works contain procurement of protection materials, temperature detection, virus kill, daily patrol and consults ect;
  2. Purchasing a batch of substance such as medicine alcohol, chlorine disinfectant, medical surgical masks, protective clothing, temperature guns for epidemic prevention;
  3. The offices, production laboratories, warehouses must be disinfected fully after daily work, and all windows must be opened;
  4. The temperature detection is needed before and after daily work for everyone, any person has the temperature over 37.3℃ must be sent to the hospital immediately. Early detection, early isolation, early treatment;
  5. All colleagues must wear mask and the colleagues in production department also must wear protective clothing, gloves, wash and change them frequently;
  6. The new purchased materials must be kept in isolated room first, and transferred into warehouses or production rooms after being thoroughly sprayed and disinfected with 75% medicine alcohol;
  7. The high temperature resistant consumables, such as bottle, pipe, experimental spear, must be sterilized via high heat and pressure, and those consumables don’t resistant to high temperature, such as box, bag, must be thoroughly sprayed and disinfected with 75% medicine alcohol to ensure the safe of products during the production;
  8. During the process of packing box, the inner and outer surface of the box must be sprayed and disinfected with 75% medicine alcohol to ensure the packaging safety;
  9. Cargos should be transported via reliable express company such as S.F. Express, FedEx, DHL to ensure the transportation safety;

Abbkine Scientific Co.,Ltd.





20th, February 2020

2020年2月20日星期四

How to Select an Antibody&Protein Coupling Kit with Super Cost Performance

1, The common definition of coupling technology





Enzymes, biotin, fluorescent dye, agarose, magnetic beads and other substances which are easy to detect or separate are covalently labeled or conjugated to antibodies, proteins or other small molecular substances by a certain technology.





2, Background of Coupling Technology





Coupling of antibodies, proteins or other small molecules is a necessary step in many types of biological experiments.





For example, in order to detect specific proteins in immunoassays (such as WB/IHC/IF and other immunological experiments), we couple primary antibody or secondary antibody with enzyme, fluorescent dye, etc.





In cell staining (biological imaging) applications, reactive dyes (fluorescent dye) are directly combined with extracellular or intracellular proteins (polypeptides) to produce visible and measurable staining.





Coupling agarose or magnetic beads to proteins or antibodies provides a targeted purification method. For example, the agarose (magnetic beads) coupled antibody used by Abbkine in IP experiments directly saves the use of Protein A/G.





3, Major Disclosure of Coupling Kits of Various Brands (Taking HRP Coupling Kits and Green Fluorescence Coupling Kits as Examples)





  1. HRP Coupling Kits
BrandSample typesContents of the kitDuration of experimentSize/Price
AbbkineSamples contain free amino group1. Activated HRP conjugates solution
2. HRP labeling solution
3. HRP quencher
3.5-4h3*20ug/$100 100ug/$120 3*100ug/$140
1mg/$220
AbcamSamples contain free amino group1. Activated HRP conjugates solution
2. HRP labeling solution
3. HRP quencher
3.5-4h30ug/¥$270
100ug/$275
300ug/$330
1mg/$555
5mg/$1887
ThermoFisherSamples contain free amino group1. Activated HRP conjugates solution
2. HRP labeling solution
3. HRP quencher
4. Protein A/G Column
overnight+>2h300µg/$652

* How does Abbkine HRP coupling kit remove free HRP (molecular weight 40KD) from binding antibody?





Abbkine HRP coupling kit was designed with this in mind. The kit can provide a low level of free HRP at the end of the reaction. Therefore, no filtration step is required, and the termination solution (quencher) provided in the kit will block the reactive groups of free HRP.





* Protein A/G column in Thermofisher brand is used to remove free HRP.





Brand Sample types Contents of the kit Duration of experiment Size/Price
Abbkine Samples contain free amino group 1. Activated Green fluorescence conjugates solution
2. labeling solution
3. Purification Column
1.5h3*20ug/$120 100ug/$140 3*100ug/$160
1mg/$250
Abcam Samples contain free amino group 1. Activated Green fluorescence conjugates solution
2. labeling solution
3. quencher
0.5-1h30ug/$265
100ug/$266
300ug/$415
1mg/$645
ThermoFisher Samples contain free amino group 1. Activated Green fluorescence conjugates solution
2. labeling solution
3. Purification Column
1.5-2h100µg/$538

* The purpose of the purification column is to separate the unconjugated dye from the coupled product, because the dye is small molecules (molecular weight is about 1KD), and the purification column volume provided by Abbkine is 50KDa, 0.5 ml. The purification column provided by ThermoFisher is also aimed at the purification of antibody (the molecular weight of antibody is about 160KD). If the molecular weight of the protein purified by customers is small or large, they need to equip themselves with appropriate ultrafiltration tubes.





The following recommendations are made for the allocation of ultrafiltration tubes:





The molecular weight of the sample Ultrafiltration tubes
6<MW<20K3K
20<MW<60K10K
60<MW<100K30K
100<MW<200K50K
200K<MW100K

To sum up, which brand of coupling kit would you choose? As the fans of Abbkine, I'd like to summarize the characteristics and advantages of Abbkine LinKine™ products:





  • Optimized Scheme-Follow the standard experimental procedure and obtain the best activity or fluorescence with excellent pre-activated dye, protein ratio and patented solution formula.
  • The operation is simple and convenient-the operation can be realized in three steps, and the coupling group is bound to a primary amine site of an antibody or other protein.
  • Customization-changing molar ratio, reaction buffer, pH value and other parameters can realize different levels of coupling and activity.
  • High Efficiency Purification-The kit includes a purification column to ensure rapid and effective removal of unreacted dyes and excellent protein/antibody recovery.

2020年2月9日星期日

Buy Abbkine selected primary antibodies, get high quality secondary antibodies

Abbkine's selected primary antibodies are the first choice for reliable trust, featuring a selection of popular research targets, strict quality control and quality assurance. We are grateful for everyone's recognition of this product line. In order to allow more customers to use good products and send out high-score articles, we are now launching the activity of buying selected primary antibodies and get high quality secondary antibodies.





Promotion time: From now to December 31th, 2020





Details of activities:





As long as you buy selected primary antibodies, you can get designated high quality secondary antibodies (size: 100ul) for free or at a very preferential price. Designated secondary antibodies are as follows,





Product nameCat#CitationSizeList Price($)Preferential Price($)
HRP, Goat Anti-Mouse IgGA21010131100ul250
HRP, Goat Anti-Rabbit IgGA21020147100ul250
DyLight 488, Goat Anti-Mouse IgGA2321079100ul4015
Dylight 488, Goat Anti-Rabbit IgGA23220107100ul4015
Dylight 549, Goat Anti-Mouse IgGA2331024100ul4015
Dylight 549, Goat Anti-Rabbit IgGA2332059100ul4015
Dylight 594, Goat Anti-Mouse IgGA2341030100ul4015
Dylight 594, Goat Anti-Rabbit IgGA2342046100ul4015
Dylight 649, Goat Anti-Mouse IgGA2361013100ul4015
Dylight 649, Goat Anti-Rabbit IgGA2362018100ul4015
Dylight 800, Goat Anti-Mouse IgGA239104100ul4015
Dylight 800, Goat Anti-Rabbit IgGA2392012100ul4015
IFKine™ Green Donkey Anti-Mouse IgGA2421111100ul7020
IFKine™ Green Donkey Anti-Rabbit IgGA2422112100ul7020
IFKine™ Red Donkey Anti-Mouse IgGA244115100ul7020
IFKine™ Red Donkey Anti-Rabbit IgGA2442122100ul7020
IPKine™ HRP, Goat Anti-Mouse IgG LCSA2501213100ul7020
IPKine™ HRP, Mouse Anti-Rabbit IgG LCSA250229100ul7020
IPKine™ HRP, Goat Anti-Mouse IgG HCSA251128100ul7020
IPKine™ HRP, Goat Anti-Rabbit IgG HCSA252227100ul7020

* IFKine™antibodies: IFKine™ are series of specially optimized secondary antibodies with improved brightness, photostability and less nonspecific hybridization and background. Donkey host and other species of serum/IgG absorbed make it ideal choice for fluorescence staining, especially in fluorescence multiple labelling.





*IPKine™ antibodies: 25 kD or 50 kD protein detection on Western blots after Immunoprecipitation is often suffered from heavy or light chain blotting contamination. Abbkine’s  IPKine™ products could solve these problems and bring you satisfying results with good performance.

Buy Abbkine selected primary antibodies, get high quality secondary antibodies

Abbkine's selected primary antibodies are the first choice for reliable trust, featuring a selection of popular research targets, strict quality control and quality assurance. We are grateful for everyone's recognition of this product line. In order to allow more customers to use good products and send out high-score articles, we are now launching the activity of buying selected primary antibodies and get high quality secondary antibodies.





Promotion time: From now to December 31th, 2020





Details of activities:





As long as you buy selected primary antibodies, you can get designated high quality secondary antibodies (size: 100ul) for free or at a very preferential price. Designated secondary antibodies are as follows,





Product nameCat#CitationSizeList Price($)Preferential Price($)
HRP, Goat Anti-Mouse IgGA21010131100ul250
HRP, Goat Anti-Rabbit IgGA21020147100ul250
DyLight 488, Goat Anti-Mouse IgGA2321079100ul4015
Dylight 488, Goat Anti-Rabbit IgGA23220107100ul4015
Dylight 549, Goat Anti-Mouse IgGA2331024100ul4015
Dylight 549, Goat Anti-Rabbit IgGA2332059100ul4015
Dylight 594, Goat Anti-Mouse IgGA2341030100ul4015
Dylight 594, Goat Anti-Rabbit IgGA2342046100ul4015
Dylight 649, Goat Anti-Mouse IgGA2361013100ul4015
Dylight 649, Goat Anti-Rabbit IgGA2362018100ul4015
Dylight 800, Goat Anti-Mouse IgGA239104100ul4015
Dylight 800, Goat Anti-Rabbit IgGA2392012100ul4015
IFKine™ Green Donkey Anti-Mouse IgGA2421111100ul7020
IFKine™ Green Donkey Anti-Rabbit IgGA2422112100ul7020
IFKine™ Red Donkey Anti-Mouse IgGA244115100ul7020
IFKine™ Red Donkey Anti-Rabbit IgGA2442122100ul7020
IPKine™ HRP, Goat Anti-Mouse IgG LCSA2501213100ul7020
IPKine™ HRP, Mouse Anti-Rabbit IgG LCSA250229100ul7020
IPKine™ HRP, Goat Anti-Mouse IgG HCSA251128100ul7020
IPKine™ HRP, Goat Anti-Rabbit IgG HCSA252227100ul7020

* IFKine™antibodies: IFKine™ are series of specially optimized secondary antibodies with improved brightness, photostability and less nonspecific hybridization and background. Donkey host and other species of serum/IgG absorbed make it ideal choice for fluorescence staining, especially in fluorescence multiple labelling.





*IPKine™ antibodies: 25 kD or 50 kD protein detection on Western blots after Immunoprecipitation is often suffered from heavy or light chain blotting contamination. Abbkine’s  IPKine™ products could solve these problems and bring you satisfying results with good performance.

2020年2月5日星期三

Wuhan Institute of Virology Reveals New Coronavirus (2019-nCoV) may Bat Origin

On February 3, the Wuhan virus research institute of the Chinese academy of sciences/biosafety research center, Wuhan jinyintan hospital and Hubei provincial center for disease prevention and control cooperated in the identification and research of 2019 new coronavirus (2019-nCoV), which caused the Wuhan pneumonia epidemic, and published it online under the topic "a pneumonia outbreak associated with a new coronavirus of disease origin" (the link of the paper is https://www.nature.com/articles/s41586-020-2012-7). Relevant research teams revealed the basic biological characteristics of the coronavirus from nucleic acid detection, serological diagnosis, virus isolation and receptor utilization, providing important clues for epidemic control and drug research and development.





The research team obtained the whole genome sequence of the virus from the early samples of 5 patients. The similarity of the virus sequence from 5 patients reached 99.9%, and the sequence consistency with SARS-CoV was 79.5%. The amino acid analysis of virus conserved protein shows that 2019-nCoV and SARS-CoV belong to SARS-related coronavirus (SARSR-COV). The author further compared 2019-nCoV genome with some gene sequences of coronavirus detected early in the laboratory, and found that the virus is similar to the gene of a coronavirus (TG13 for short) derived from a bat sample. After sequencing the bat sample, the whole genome sequence of bat virus TG13 was obtained, and the sequence consistency of the two viruses was found as high as 96% (Figure 1).





Fig. 1. high-throughput sequencing obtains the whole genome sequence (A) of the virus from a patient sample; Sketch map of 2019-nCoV genome (b); Homology analysis with human SARS virus and bat SARSr-CoV (c); Evolutionary analysis using 2019-nCoV replicase gene (d)





The research team carried out nucleic acid detection on samples of bronchoalveolar lavage fluid (BALF), throat swab (OS) and other samples of patients in different periods, and found that the viral nucleic acid of early samples was about 1000 times higher than that of late samples. By using SARSr-CoV antigen stored in the laboratory at an early stage, they tested the patient's serum samples and found that the patient produced corresponding IgM and IgG antibodies (fig. 2). Subsequently, 2019-nCoV virus was isolated from bronchoalveolar lavage fluid of a critically ill patient, and its intracellularly clear coronavirus particle morphology was observed by electron microscopy (fig. 3).





Fig. 2. virus nucleic acid detection in seven patients (a); Serological changes in a patient (B); Detection of Antibody Level in Seven Patients (C)





Fig. 3. electron microscopic observation of 2019-ncov in cells





The research team found that 2019-nCoV can infect non-sensitive cells that express human ACE2 (angiotensin converting enzyme, cell receptor of SARS-CoV), indicating that 2019-nCoV can invade cells using receptor of SARS-CoV (fig. 4).





Fig. 4. 2019-nCoV infection in HeLa cells expressing ACE2 from human, bat, pig, civet and mouse





In the race against time and epidemic situation, the study provided more detailed basic biological characteristics of 2019-nCoV virus in a short period of time, and revealed that the natural host of the virus may be bats. The study also showed that 2019-nCoV can infect cells with the receptor of SARS-CoV, laying a working foundation for subsequent epidemic prevention and drug research.





Product Recommendation:





  1. ACE2 Polyclonal Antibody(ABP56008)
  2. DyLight 488, Goat Anti-Mouse IgG (A23210)
  3. Dylight 488, Goat Anti-Rabbit IgG (A23220)
  4. Dylight 549, Goat Anti-Mouse IgG (A23310)
  5. Dylight 549, Goat Anti-Rabbit IgG (A23320)
  6. Dylight 594, Goat Anti-Mouse IgG (A23410)
  7. Dylight 594, Goat Anti-Rabbit IgG (A23420)