Western Blot Product Portfolio

Based on Abbkine Proteomics Research Workstation, we provide Western Blot (WB) product portfolio for global scientific researchers.

Abbkine scientists have been devoted to immunology and cytology research, developing and providing high-quality products in order to accelerate life science research. We provide global life science researchers with the basic research tools at reasonable prices and excellent quality to advance human and animal health research.

Our most popular WB related products are listed in this manual, you can also visit www.abbkine.com to view our complete products. Abbkine will continue to develop more WB related products.[pdfjs-viewer url="https%3A%2F%2Fwww.abbkine.com%2Fwp-content%2Fuploads%2F2020%2F06%2FWestern-Blot-Product-Portfolio.pdf" viewer_width=100% viewer_height=1360px fullscreen=true download=true print=true]

Abbkine Newly Released Selected Primary Antibody

Abbkine focuses on Proteomics and Cytology, and is committed to the innovation and development of various scientific kits, proteins, antibodies and other research tools related to proteomics and cytology, expecting to accelerate the pace of life science research and drug discovery. Here, we present our newly launched selected primary antibodies, which involve hot target research areas, with high quality and bulk stock.

[pdfjs-viewer url="https%3A%2F%2Fwww.abbkine.com%2Ffile%2Fdistributor%2FNewly Released Selected Primary Antibody.pdf" viewer_width=100% viewer_height=950px fullscreen=true download=false print=true]

New product | Focusing on glutathione metabolism- Abbkine new product coming in May!

It's time to release new products every month, so it's exciting to think about it! After the hard work of our R & D personnel, Abbkine welcomed 7 new partners in May! The new product line of this issue-glutathione series biochemical kits.

The significance of glutathione research:

Glutathione is a
tripeptide containing glutamate, cysteine and glycine, which contains
sulfhydryl groups. It is widely present in animals and plants. It has
antioxidant and integrated detoxification effects. Its related coenzyme is
NADPH.

Too much free
radicals produced by the body's metabolism can damage the biofilm, invade
macromolecules of life, accelerate the body's aging, and induce the production
of tumors or atherosclerosis. Glutathione has the functions of enhancing the
antioxidant function of organisms, promoting growth, and enhancing the
influence of organisms on the adverse environment in the human body. Its main
physiological role is to remove free radicals in the body. It is also a good
antidote, which can weaken the toxicity of toxic substances and remove toxic
substances from the body. Glutathione also promotes growth and improves human
immunity.

Glutathione can
be used not only as a medicine but also as a base material for functional
foods, and is widely used in functional foods such as anti-aging, enhancing
immunity and anti-tumor.

Interpretation of
new products：

1) CheKine^TM Reduced Glutathione (GSH) Colorimetric Assay Kit: Application notes DTNB reacts with reduced glutathione to form a yellow product. The optical density measured at 412 nm, can directly reflect glutathione concentration in the sample.

2) CheKine^TM
Glutathione Oxidized (GSSG) Colorimetric Assay Kit: Reduced glutathione can
react with DTNB and generate 2-nitro-5-mercaptobenzoic acid, which has the
maximum light absorption at 412nm wavelength.

3) CheKine^TM
Glutathione Reductases (GR) Colorimetric Assay Kit: GR can catalyze the reduction
of NADPH to GSSG to regenerate GSH, and NADPH dehydrogenates to produce NADP +;
NADPH has a characteristic absorption peak at 340 nm, while NADP + has no
absorption peak at this wavelength; NADPH dehydrogenation rate is determined by
measuring the decrease rate of absorbance at 340 nm to calculate GR activity.

4) CheKine^TM
Glutathione S-Transferase (GST) Colorimetric Assay Kit: GST catalyzes the
binding of GSH to CDNB. The conjugation is accompanied by an increase in
absorbance at 340 nm. The rate of increase is directly proportional to the GST
activity in the sample.

5) CheKine^TM
Glutathione Peroxidase (GSH-Px) Colorimetric Assay Kit: GSH-Px catalyzes H₂O₂
to oxidize GSH to produce GSSG; glutathione reductase (GR) catalyzes NADPH to
reduce GSSG to regenerate GSH, while NADPH oxidizes to produce NADP+; NADPH has
a characteristic absorption peak at 340 nm, while NADP+ does not; NADPH
dehydrogenation rate is determined by measuring the decrease rate of absorbance
at 340 nm to calculate GSH-Px activity.

6) CheKine^TM
Thioredoxin Reductase (TrxR) Colorimetric Assay Kit: TrxR can catalyzes the
reduction of DTNB by NADPH to generate TNB and NADP+. TNB has a characteristic
absorption peak at 412 nm. TrxR activity can be calculated by measuring the
increase rate of TNB at 412 nm.

7) CheKine^TM
Thioredoxin Peroxidase (TPX) Colorimetric Assay Kit: TPX catalyzes H₂O₂
to oxidize dithiothreitol (DTT). The absorption wavelength of H₂O₂
is 240nm. TPX activity can be calculated by measuring the decrease rate of
absorbance at 240nm and subtracting H₂O₂ catalyzed by
catalase (CAT). Therefore, this kit can measure TPX and CAT activities of
samples simultaneously.

New product list:

CAT#	Product name	classification
KTB1600	CheKine™ Reduced Glutathione (GSH) Colorimetric Assay Kit	Cell metabolism
KTB1610	CheKine™ Glutathione Oxidized (GSSG) Colorimetric Assay Kit	Cell metabolism
KTB1620	CheKine™ Glutathione Reductases (GR) Colorimetric Assay Kit	Cell metabolism
KTB1630	CheKine™ Glutathione S-Transferase (GST) Colorimetric Assay Kit	Cell metabolism
KTB1640	CheKine™ Glutathione Peroxidase (GSH-Px) Colorimetric Assay Kit	Cell metabolism
KTB1650	CheKine™ Thioredoxin Reductase (TrxR) Colorimetric Assay Kit	Cell metabolism
KTB1660	CheKine™ Thioredoxin Peroxidase (TPX) Colorimetric Assay Kit	Cell metabolism

Abbkine focuses on the fields of immunology and cytology, and is committed to innovating and developing various types of antibodies, proteins, analytical reagents and kits, with a view to becoming a key promoter in the fields of life science research and development, and drug development. Here, we present to you the favorite products of protein and immune research users, from basic immunological products, such as protein extraction and quantification, to internal reference label antibodies, primary and secondary antibodies for immunological experiments; cell research users’ favorite Products ranging from dyes and kits for cell state detection, organelle extraction kits, cell substructure staining and cell metabolism detection products, to cytokine and protein detection kits for cell culture, just to help you Research career!

About Abbkine
Scientific Co., Ltd.

Abbkine
Scientific Co., Ltd. was founded in 2012. Our mission is to become a respected
and world-class supplier of biomedical products and services. Through a clear
core strategy and a corporate culture that promotes learning, innovation,
cooperation, and excellence, we will stimulate our inherent creativity, provide
competitive biomedical products and services, and continue to create maximum
value for customers and achieve our mission.

Hard work, Do not forget your initiative mind- Abbkine Added 210 Citations in April

In April 2020, Abbkine collected 210 English
articles published using Abbkine products, and some of them sent us good news
on their own initiative. The joy was beyond words. In this collection of
articles, the highest impact factor (IF) is 13.05, with articles with IF>7
accounting for 10.4% and articles with IF>4 accounting for 43%.

In the current evaluation of scientific
research, scientific research papers, as the systematic summary and theoretical
crystallization of scientific research carried out by scientific researchers,
are still the main objects for measuring innovative activities, especially
basic research activities. Science, Nature and Cell are internationally
recognized journals with the highest academic reputation. According to the
statistical results of Chinese scientific papers in 2018, the number of Chinese
papers published in the above three journals in 2017 was 309, an increase of 11
articles over the previous year, ranking fourth in the world. China's
scientific research strength should not be underestimated!

Let's take a look at some of the research
workers in China who are studying some topics.

Title: MST4 kinase suppresses
gastric tumorigenesis by limiting YAP activation via a non-canonical pathway

Magazine: Journal of
Experimental Medicine

Impact
factor:
9.83

University:
Tongji University

Abstract: Hyperactivation of YAP has been commonly associated with tumorigenesis,
and emerging evidence hints at multilayered Hippo-independent regulations of
YAP. In this study, we identified a new MST4–YAP
axis, which acts as a noncanonical Hippo signaling pathway that limits
stress-induced YAP activation. MST4 kinase directly phosphorylated YAP at Thr83
to block its binding with importin α, therefore leading to YAP cytoplasmic
retention and inactivation. Due to a consequential interplay between
MST4-mediated YAP phospho-Thr83 signaling and the classical YAP phospho-Ser127
signaling, the phosphorylation level of YAP at Thr83 was correlated to that at
Ser127. Mutation of T83E mimicking MST4-mediated alternative signaling
restrained the activity of both wild-type YAP and its S127A mutant mimicking
loss of classical Hippo signal. Depletion of MST4 in mice promoted gastric
tumorigenesis with diminished Thr83 phosphorylation and hyperactivation of YAP.
Moreover, loss of MST4–YAP signaling was associated with poor
prognosis of human gastric cancer. Collectively, our study uncovered a
noncanonical MST4–YAP signaling axis essential for suppressing
gastric tumorigenesis.

Article link: https://rupress.org/jem/article/217/6/e20191817/151647

Products
using from Abbkine:

 IPKine™ HRP, Goat Anti-Mouse
IgG LCS (CAT#A25012)

IPKine™
HRP, Mouse Anti-Rabbit IgG LCS (CAT#A25022)

Title:
TRIM32/USP11
Balances ARID1A Stability and the Oncogenic/Tumor-Suppressive Status of
Squamous Cell Carcinoma

Magazine: Cell Reports

Impact
factor:
8.04

University:
Chinese
Academy of Medical Sciences and Peking Union Medical College

Abstract: Squamous cell carcinoma (SCC) is an
aggressive epithelial malignancy, yet the molecular mechanisms underlying SCC
development are elusive. ARID1A is frequently mutated in various cancer types,
but both mutation rates and expression levels of ARID1A are ubiquitously low in
SCCs. Here, we reveal that excessive protein degradation mediated by the
ubiquitin-proteasome system (UPS) contributes to the loss of ARID1A expression
in SCC. We identify that the E3 ligase TRIM32 and the deubiquitinase USP11 play
key roles in controlling ARID1A stability. TRIM32 depletion inhibits SCC cell
proliferation, metastasis, and chemoresistance by stabilizing ARID1A, while
USP11 depletion promotes SCC development by promoting ARID1A degradation. We
show that syndecan-2 (SDC2) is the downstream target of both ARID1A and USP11
and that SDC2 depletion abolishes the oncogenic function of ARID1A loss. In
summary, our data reveal UPS-mediated protein degradation as a mechanism
underlying ARID1A loss and propose an important role for the
TRIM32/USP11-ARID1A-SDC2 axis in SCC.

Article link: https://www.sciencedirect.com/science/article/pii/S2211124719316675

Products
using from Abbkine:

ExKine^TM
Nuclear and Cytoplasmic Protein Extraction Kit (CAT#KTP3001)

Title:
Microneedle
drug eluting balloon for enhanced drug delivery to vascular tissue

Magazine:
Journal
of Controlled Release

Impact
factor: 7.82

University:
Yonsei
University, Seoul

Abstract: High rates of restenosis and neointimal
formation have driven increasing interest in the application of drug eluting
balloons (DEB) as counteractive measures for intraluminal drug delivery. The
use of DEBs eliminates the need for stents so that serious side effects including
in-stent restenosis and stent thrombosis can be avoided and long-term
medication of antiplatelet agent is not needed. Despite their benefits, DEBs
have poor drug delivery efficiency due to short balloon inflation times (30~60
seconds) that limit the passive drug diffusion from the balloon surface to the
luminal lesion. To increase drug delivery efficiency, a microneedle DEB (MNDEB)
was developed by a conformal transfer molding process using a thin polydimethylsiloxane
mold bearing a negative array of MNs of 200 μm in height. A MN
array composed of UV curable resin was formed onto the surface of DEB, and
drugs were coated onto the structure. The mechanical properties of the MN array
were investigated and MN

penetration
into luminal vasculature was confirmed in vivo. An increase in drug delivery efficiency
compared to a standard DEB was demonstrated in an in vivo test in a rabbit
aorta. Finally, the superior therapeutic efficacy of MNDEBs was evaluated using
an atherosclerosis rabbit model.

Article link: https://www.sciencedirect.com/science/article/abs/pii/S0168365920300894

Products
using from Abbkine:

EliKine™
Mouse IL-1β
ELISA Kit (CAT#KET7005)

EliKine™
Mouse TNF-α
ELISA Kit (CAT#KET7015)

Title:
Polyphyllin
VI Induces Caspase-1-Mediated Pyroptosis via the Induction of ROS/NF-κB/NLRP3/GSDMD
Signal Axis in Non-Small Cell Lung Cancer

Magazine:
Cancers

Impact
factor: 5.87

University:
Southwest
Medical University

Abstract: Trillium tschonoskii Maxim
(TTM), a traditional Chinese medicine, has been demonstrated to have a potent
anti-tumor effect. Recently, polyphyllin VI (PPVI), a main saponin isolated
from TTM, was reported by us to significantly suppress the proliferation of
non-small cell lung cancer (NSCLC) via the induction of apoptosis and autophagy
in vitro and in vivo. In this study, we further found that the NLRP3
inflammasome was activated in PPVI administrated A549-bearing athymic nude
mice. As is known to us, pyroptosis is an inflammatory form of
caspase-1-dependent programmed cell death that plays an important role in
cancer. By using A549 and H1299 cells, the in vitro effect and action mechanism
by which PPVI induces activation of the NLRP3 inflammasome in NSCLC were
investigated. The anti-proliferative effect of PPVI in A549 and H1299 cells was
firstly measured and validated by MTT assay. The activation of the NLRP3
inflammasome was detected by using Hoechst33324/PI staining, flow cytometry
analysis and real-time live cell imaging methods. We found that PPVI
significantly increased the percentage of cells with PI signal in A549 and
H1299, and the dynamic change in cell morphology and the process of cell death
of A549 cells indicated that PPVI induced an apoptosis-to-pyroptosis switch,
and, ultimately, lytic cell death. In addition, belnacasan (VX-765), an
inhibitor of caspase-1, could remarkably decrease the pyroptotic cell death of
PPVI-treated A549 and H1299 cells. Moreover, by detecting the expression of
NLRP3, ASC, caspase-1, IL-1β, IL-18 and GSDMD in A549 and h1299 cells using Western
blotting, immunofluorescence imaging and flow cytometric analysis, measuring
the caspase-1 activity using colorimetric assay, and quantifying the cytokines
level of IL-1β and
IL-18 using ELISA, the NLRP3 inflammasome was found to be activated in a dose
manner, while VX-765 and necrosulfonamide (NSA), an inhibitor of GSDMD, could
inhibit PPVI-induced activation of the NLRP3 inflammasome. Furthermore, the
mechanism study found that PPVI could activate the NF-κB signaling pathway via increasing reactive
oxygen species (ROS) levels in A549 and H1299 cells, and N-acetyl-L-cysteine
(NAC), a scavenger of ROS, remarkably inhibited the cell death, and the
activation of NF-κB and the NLRP3 inflammasome in PPVI-treated A549 and H1299
cells. Taken together, these data suggested that PPVI-induced,
caspase-1-mediated pyroptosis via the induction of the ROS/NF-κB/NLRP3/GSDMD
signal axis in NSCLC, which further clarified the mechanism of PPVI in the
inhibition of NSCLC, and thereby provided a possibility for PPVI to serve as a
novel therapeutic agent for NSCLC in the future

Article link: https://www.mdpi.com/2072-6694/12/1/193

Products
using from Abbkine:

Caspase-1
Assay Kit (Colorimetric) (CAT#KTA3020)

Title:
Integrin
β3 promotes cardiomyocyte proliferation
and attenuates hypoxia-induced apoptosis via regulating the PTEN/Akt/mTOR and
ERK1/2 pathways

Magazine: International
Journal of Biological Sciences

Impact
factor:
4.04

University:
Shanghai
Jiaotong University

Article link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6990915/

Abstract: Integrin β3 is one
of the main integrin heterodimer receptors on the surface of cardiac myocytes.
Our previous studies showed that hypoxia induces apoptosis and increases
integrin β3
expression in cardiomyocytes. However, the exact mechanism by which integrin β3 protects
against apoptosis remains unclear. Hence, the present investigation aimed to
explore the mechanism of integrin β3 in cardiomyocyte proliferation and
hypoxia-induced cardiomyocyte apoptosis.

Products
using from Abbkine:

Annexin V-AbFluor™ 555 Apoptosis Detection kit (CAT#KTA0003)

Hard work, Do not forget your initiative mind- Abbkine Added 210 Citations in April

In April 2020, Abbkine collected 210 English
articles published using Abbkine products, and some of them sent us good news
on their own initiative. The joy was beyond words. In this collection of
articles, the highest impact factor (IF) is 13.05, with articles with IF>7
accounting for 10.4% and articles with IF>4 accounting for 43%.

In the current evaluation of scientific
research, scientific research papers, as the systematic summary and theoretical
crystallization of scientific research carried out by scientific researchers,
are still the main objects for measuring innovative activities, especially
basic research activities. Science, Nature and Cell are internationally
recognized journals with the highest academic reputation. According to the
statistical results of Chinese scientific papers in 2018, the number of Chinese
papers published in the above three journals in 2017 was 309, an increase of 11
articles over the previous year, ranking fourth in the world. China's
scientific research strength should not be underestimated!

Let's take a look at some of the research
workers in China who are studying some topics.

Title: MST4 kinase suppresses
gastric tumorigenesis by limiting YAP activation via a non-canonical pathway

Magazine: Journal of
Experimental Medicine

Impact
factor:
9.83

University:
Tongji University

Abstract: Hyperactivation of YAP has been commonly associated with tumorigenesis,
and emerging evidence hints at multilayered Hippo-independent regulations of
YAP. In this study, we identified a new MST4–YAP
axis, which acts as a noncanonical Hippo signaling pathway that limits
stress-induced YAP activation. MST4 kinase directly phosphorylated YAP at Thr83
to block its binding with importin α, therefore leading to YAP cytoplasmic
retention and inactivation. Due to a consequential interplay between
MST4-mediated YAP phospho-Thr83 signaling and the classical YAP phospho-Ser127
signaling, the phosphorylation level of YAP at Thr83 was correlated to that at
Ser127. Mutation of T83E mimicking MST4-mediated alternative signaling
restrained the activity of both wild-type YAP and its S127A mutant mimicking
loss of classical Hippo signal. Depletion of MST4 in mice promoted gastric
tumorigenesis with diminished Thr83 phosphorylation and hyperactivation of YAP.
Moreover, loss of MST4–YAP signaling was associated with poor
prognosis of human gastric cancer. Collectively, our study uncovered a
noncanonical MST4–YAP signaling axis essential for suppressing
gastric tumorigenesis.

Article link: https://rupress.org/jem/article/217/6/e20191817/151647

Products
using from Abbkine:

 IPKine™ HRP, Goat Anti-Mouse
IgG LCS (CAT#A25012)

IPKine™
HRP, Mouse Anti-Rabbit IgG LCS (CAT#A25022)

Title:
TRIM32/USP11
Balances ARID1A Stability and the Oncogenic/Tumor-Suppressive Status of
Squamous Cell Carcinoma

Magazine: Cell Reports

Impact
factor:
8.04

University:
Chinese
Academy of Medical Sciences and Peking Union Medical College

Abstract: Squamous cell carcinoma (SCC) is an
aggressive epithelial malignancy, yet the molecular mechanisms underlying SCC
development are elusive. ARID1A is frequently mutated in various cancer types,
but both mutation rates and expression levels of ARID1A are ubiquitously low in
SCCs. Here, we reveal that excessive protein degradation mediated by the
ubiquitin-proteasome system (UPS) contributes to the loss of ARID1A expression
in SCC. We identify that the E3 ligase TRIM32 and the deubiquitinase USP11 play
key roles in controlling ARID1A stability. TRIM32 depletion inhibits SCC cell
proliferation, metastasis, and chemoresistance by stabilizing ARID1A, while
USP11 depletion promotes SCC development by promoting ARID1A degradation. We
show that syndecan-2 (SDC2) is the downstream target of both ARID1A and USP11
and that SDC2 depletion abolishes the oncogenic function of ARID1A loss. In
summary, our data reveal UPS-mediated protein degradation as a mechanism
underlying ARID1A loss and propose an important role for the
TRIM32/USP11-ARID1A-SDC2 axis in SCC.

Article link: https://www.sciencedirect.com/science/article/pii/S2211124719316675

Products
using from Abbkine:

ExKine^TM
Nuclear and Cytoplasmic Protein Extraction Kit (CAT#KTP3001)

Title:
Microneedle
drug eluting balloon for enhanced drug delivery to vascular tissue

Magazine:
Journal
of Controlled Release

Impact
factor: 7.82

University:
Yonsei
University, Seoul

Abstract: High rates of restenosis and neointimal
formation have driven increasing interest in the application of drug eluting
balloons (DEB) as counteractive measures for intraluminal drug delivery. The
use of DEBs eliminates the need for stents so that serious side effects including
in-stent restenosis and stent thrombosis can be avoided and long-term
medication of antiplatelet agent is not needed. Despite their benefits, DEBs
have poor drug delivery efficiency due to short balloon inflation times (30~60
seconds) that limit the passive drug diffusion from the balloon surface to the
luminal lesion. To increase drug delivery efficiency, a microneedle DEB (MNDEB)
was developed by a conformal transfer molding process using a thin polydimethylsiloxane
mold bearing a negative array of MNs of 200 μm in height. A MN
array composed of UV curable resin was formed onto the surface of DEB, and
drugs were coated onto the structure. The mechanical properties of the MN array
were investigated and MN

penetration
into luminal vasculature was confirmed in vivo. An increase in drug delivery efficiency
compared to a standard DEB was demonstrated in an in vivo test in a rabbit
aorta. Finally, the superior therapeutic efficacy of MNDEBs was evaluated using
an atherosclerosis rabbit model.

Article link: https://www.sciencedirect.com/science/article/abs/pii/S0168365920300894

Products
using from Abbkine:

EliKine™
Mouse IL-1β
ELISA Kit (CAT#KET7005)

EliKine™
Mouse TNF-α
ELISA Kit (CAT#KET7015)

Title:
Polyphyllin
VI Induces Caspase-1-Mediated Pyroptosis via the Induction of ROS/NF-κB/NLRP3/GSDMD
Signal Axis in Non-Small Cell Lung Cancer

Magazine:
Cancers

Impact
factor: 5.87

University:
Southwest
Medical University

Abstract: Trillium tschonoskii Maxim
(TTM), a traditional Chinese medicine, has been demonstrated to have a potent
anti-tumor effect. Recently, polyphyllin VI (PPVI), a main saponin isolated
from TTM, was reported by us to significantly suppress the proliferation of
non-small cell lung cancer (NSCLC) via the induction of apoptosis and autophagy
in vitro and in vivo. In this study, we further found that the NLRP3
inflammasome was activated in PPVI administrated A549-bearing athymic nude
mice. As is known to us, pyroptosis is an inflammatory form of
caspase-1-dependent programmed cell death that plays an important role in
cancer. By using A549 and H1299 cells, the in vitro effect and action mechanism
by which PPVI induces activation of the NLRP3 inflammasome in NSCLC were
investigated. The anti-proliferative effect of PPVI in A549 and H1299 cells was
firstly measured and validated by MTT assay. The activation of the NLRP3
inflammasome was detected by using Hoechst33324/PI staining, flow cytometry
analysis and real-time live cell imaging methods. We found that PPVI
significantly increased the percentage of cells with PI signal in A549 and
H1299, and the dynamic change in cell morphology and the process of cell death
of A549 cells indicated that PPVI induced an apoptosis-to-pyroptosis switch,
and, ultimately, lytic cell death. In addition, belnacasan (VX-765), an
inhibitor of caspase-1, could remarkably decrease the pyroptotic cell death of
PPVI-treated A549 and H1299 cells. Moreover, by detecting the expression of
NLRP3, ASC, caspase-1, IL-1β, IL-18 and GSDMD in A549 and h1299 cells using Western
blotting, immunofluorescence imaging and flow cytometric analysis, measuring
the caspase-1 activity using colorimetric assay, and quantifying the cytokines
level of IL-1β and
IL-18 using ELISA, the NLRP3 inflammasome was found to be activated in a dose
manner, while VX-765 and necrosulfonamide (NSA), an inhibitor of GSDMD, could
inhibit PPVI-induced activation of the NLRP3 inflammasome. Furthermore, the
mechanism study found that PPVI could activate the NF-κB signaling pathway via increasing reactive
oxygen species (ROS) levels in A549 and H1299 cells, and N-acetyl-L-cysteine
(NAC), a scavenger of ROS, remarkably inhibited the cell death, and the
activation of NF-κB and the NLRP3 inflammasome in PPVI-treated A549 and H1299
cells. Taken together, these data suggested that PPVI-induced,
caspase-1-mediated pyroptosis via the induction of the ROS/NF-κB/NLRP3/GSDMD
signal axis in NSCLC, which further clarified the mechanism of PPVI in the
inhibition of NSCLC, and thereby provided a possibility for PPVI to serve as a
novel therapeutic agent for NSCLC in the future

Article link: https://www.mdpi.com/2072-6694/12/1/193

Products
using from Abbkine:

Caspase-1
Assay Kit (Colorimetric) (CAT#KTA3020)

Title:
Integrin
β3 promotes cardiomyocyte proliferation
and attenuates hypoxia-induced apoptosis via regulating the PTEN/Akt/mTOR and
ERK1/2 pathways

Magazine: International
Journal of Biological Sciences

Impact
factor:
4.04

University:
Shanghai
Jiaotong University

Article link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6990915/

Abstract: Integrin β3 is one
of the main integrin heterodimer receptors on the surface of cardiac myocytes.
Our previous studies showed that hypoxia induces apoptosis and increases
integrin β3
expression in cardiomyocytes. However, the exact mechanism by which integrin β3 protects
against apoptosis remains unclear. Hence, the present investigation aimed to
explore the mechanism of integrin β3 in cardiomyocyte proliferation and
hypoxia-induced cardiomyocyte apoptosis.

Products
using from Abbkine:

Annexin V-AbFluor™ 555 Apoptosis Detection kit (CAT#KTA0003)

New product： IL-6, TNF-alpha, bFGF, IFN-gamma recombinant protein upgrading!

The new product line in this recombinant protein series:

Human
IL-6 protein：Interleukin 6 (IL-6) is a pleiotropic
cytokine that plays an important role in host defense by regulating immune and
inflammatory responses. IL-6 is an important member of the cytokine network and
is central to the acute inflammatory response. After the production of IL-6, it
induces the production of C-reactive protein (CRP) and procalcitonin (PCT),
which are associated with inflammatory diseases and infections. The degree is
directly related. IL-6 can quickly diagnose early inflammation and warn of
sepsis.

Human
TNF-alpha protein：Tumor necrosis factor-α (TNF-α) is a cytokine
involved in systemic inflammation, mainly secreted by macrophages. Its main function
is to regulate immune cells. As an endogenous pyrogen, it can cause fever,
cause apoptosis, prevent tumorigenesis and virus replication. Its dysfunction
is thought to be related to many diseases, such as Alzheimer's disease,
psoriasis, cancer, major depression, and enteritis. TNF-a is an important part
of the cell signaling pathway, so it has become the target of multiple drugs.

Human
bFGF protein、Mouse bFGF protein：

Basic fibroblast growth factor (bFGF) is a trace
substance present in mammals and a multifunctional cell growth factor. bFGF has
obvious tendency activity to wound cells, induces inflammatory cells,
fibroblasts, vascular endothelial cells to move to the wound site, activates
the phagocytic function of macrophages, improves the body's immune activity,
and significantly reduces the chance of wound infection.

Human
IFN-gamma protein, His Tag、Mouse IFN-gamma protein, His Tag：IFN-gamma belongs
to the type II interferon family， which is
widely involved in immune and inflammatory responses. Proper activation of IFN-γ signaling pathway can promote the activation
of macrophages and mediate the host's defense function against pathogens. At
the same time, IFN-γ signaling
pathway can also play an immunomodulatory role in anti-tumor immunity.

New product details:

Product supply form: freeze-dried powder
form, very stable at room temperature. Recommendation: Lyophilized protein
product should be stored desiccated below -20°C.. After dissolution, the
product can be stored at 4℃ for 2-7 days, please keep it below -20℃ for long-term
use. For long-term storage, it is recommended to add carrier protein (0.1% HSA
or BSA), and store it separately as appropriate to avoid repeated freezing and
thawing.

Application areas: antibody preparation or
detection, ELISA standards, cell or tissue culture and other functional
studies.

Price guarantee: better discounts, lower prices.

Product name	CAT#	Preparation method	Purity	Formulation	Size/ Price	Fig. SDS-PAGE
Human IL-6 protein	PRP1012	E. coli	> 95 %	Lyophilized powder	5ug/$48 20ug/$120 100ug/$300 1mg/$800
Human TNF-alpha protein	PRP1013	E. coli	> 95 %	Lyophilized powder	10ug/$48 50ug/$120 100ug/$170 1mg/$800
Human bFGF protein	PRP1010	E. coli	> 95 %	Lyophilized powder	10ug/$48 50ug/$120 100ug/$170 1mg/$900
Mouse bFGF protein	PRP1011	E. coli	> 95 %	Lyophilized powder	10ug/$48 50ug/$120 100ug/$170 1mg/$900
Human IFN-gamma protein, His Tag	PRP1014	E. coli	> 95 %	Lyophilized powder	20ug/$48 100ug/$120 500ug/$450 1mg/$800
Mouse IFN-gamma protein, His Tag	PRP1015	E. coli	> 95 %	Lyophilized powder	20ug/$48 100ug/$120 500ug/$450 1mg/$800

Abbkine focuses on the fields of immunology
and cytology, and is committed to innovating and developing various types of
antibodies, proteins, analytical reagents and kits, with a view to becoming a
key promoter in the fields of life science research and development, and drug
development. Here, we present to you the favorite products of protein and
immune research users, from basic immunological products, such as protein
extraction and quantification, to internal reference label antibodies, primary
and secondary antibodies for immunological experiments; cell research users’
favorite Products ranging from dyes and kits for cell state detection,
organelle extraction kits, cell substructure staining and cell metabolism
detection products, to cytokine and protein detection kits for cell culture,
just to help you Research career!

About Abbkine Scientific Co., Ltd.

Abbkine Scientific Co., Ltd. was founded in
2012. Our mission is to become a respected and world-class supplier of
biomedical products and services. Through a clear core strategy and a corporate culture that
promotes learning, innovation, cooperation, and excellence, we will stimulate
our inherent creativity, provide competitive biomedical products and services,
and continue to create maximum value for customers and achieve our mission.

New product： IL-6, TNF-alpha, bFGF, IFN-gamma recombinant protein upgrading!

The new product line in this recombinant protein series:

Human
IL-6 protein：Interleukin 6 (IL-6) is a pleiotropic
cytokine that plays an important role in host defense by regulating immune and
inflammatory responses. IL-6 is an important member of the cytokine network and
is central to the acute inflammatory response. After the production of IL-6, it
induces the production of C-reactive protein (CRP) and procalcitonin (PCT),
which are associated with inflammatory diseases and infections. The degree is
directly related. IL-6 can quickly diagnose early inflammation and warn of
sepsis.

Human
TNF-alpha protein：Tumor necrosis factor-α (TNF-α) is a cytokine
involved in systemic inflammation, mainly secreted by macrophages. Its main function
is to regulate immune cells. As an endogenous pyrogen, it can cause fever,
cause apoptosis, prevent tumorigenesis and virus replication. Its dysfunction
is thought to be related to many diseases, such as Alzheimer's disease,
psoriasis, cancer, major depression, and enteritis. TNF-a is an important part
of the cell signaling pathway, so it has become the target of multiple drugs.

Human
bFGF protein、Mouse bFGF protein：

Basic fibroblast growth factor (bFGF) is a trace
substance present in mammals and a multifunctional cell growth factor. bFGF has
obvious tendency activity to wound cells, induces inflammatory cells,
fibroblasts, vascular endothelial cells to move to the wound site, activates
the phagocytic function of macrophages, improves the body's immune activity,
and significantly reduces the chance of wound infection.

Human
IFN-gamma protein, His Tag、Mouse IFN-gamma protein, His Tag：IFN-gamma belongs
to the type II interferon family， which is
widely involved in immune and inflammatory responses. Proper activation of IFN-γ signaling pathway can promote the activation
of macrophages and mediate the host's defense function against pathogens. At
the same time, IFN-γ signaling
pathway can also play an immunomodulatory role in anti-tumor immunity.

New product details:

Product supply form: freeze-dried powder
form, very stable at room temperature. Recommendation: Lyophilized protein
product should be stored desiccated below -20°C.. After dissolution, the
product can be stored at 4℃ for 2-7 days, please keep it below -20℃ for long-term
use. For long-term storage, it is recommended to add carrier protein (0.1% HSA
or BSA), and store it separately as appropriate to avoid repeated freezing and
thawing.

Application areas: antibody preparation or
detection, ELISA standards, cell or tissue culture and other functional
studies.

Price guarantee: better discounts, lower prices.

Product name	CAT#	Preparation method	Purity	Formulation	Size/ Price	Fig. SDS-PAGE
Human IL-6 protein	PRP1012	E. coli	> 95 %	Lyophilized powder	5ug/$48 20ug/$120 100ug/$300 1mg/$800
Human TNF-alpha protein	PRP1013	E. coli	> 95 %	Lyophilized powder	10ug/$48 50ug/$120 100ug/$170 1mg/$800
Human bFGF protein	PRP1010	E. coli	> 95 %	Lyophilized powder	10ug/$48 50ug/$120 100ug/$170 1mg/$900
Mouse bFGF protein	PRP1011	E. coli	> 95 %	Lyophilized powder	10ug/$48 50ug/$120 100ug/$170 1mg/$900
Human IFN-gamma protein, His Tag	PRP1014	E. coli	> 95 %	Lyophilized powder	20ug/$48 100ug/$120 500ug/$450 1mg/$800
Mouse IFN-gamma protein, His Tag	PRP1015	E. coli	> 95 %	Lyophilized powder	20ug/$48 100ug/$120 500ug/$450 1mg/$800

Abbkine focuses on the fields of immunology
and cytology, and is committed to innovating and developing various types of
antibodies, proteins, analytical reagents and kits, with a view to becoming a
key promoter in the fields of life science research and development, and drug
development. Here, we present to you the favorite products of protein and
immune research users, from basic immunological products, such as protein
extraction and quantification, to internal reference label antibodies, primary
and secondary antibodies for immunological experiments; cell research users’
favorite Products ranging from dyes and kits for cell state detection,
organelle extraction kits, cell substructure staining and cell metabolism
detection products, to cytokine and protein detection kits for cell culture,
just to help you Research career!

About Abbkine Scientific Co., Ltd.

Abbkine Scientific Co., Ltd. was founded in
2012. Our mission is to become a respected and world-class supplier of
biomedical products and services. Through a clear core strategy and a corporate culture that
promotes learning, innovation, cooperation, and excellence, we will stimulate
our inherent creativity, provide competitive biomedical products and services,
and continue to create maximum value for customers and achieve our mission.

New product： IL-6, TNF-alpha, bFGF, IFN-gamma recombinant protein upgrading

The new product line in this recombinant protein series:

Human
IL-6 protein：Interleukin 6 (IL-6) is a pleiotropic
cytokine that plays an important role in host defense by regulating immune and
inflammatory responses. IL-6 is an important member of the cytokine network and
is central to the acute inflammatory response. After the production of IL-6, it
induces the production of C-reactive protein (CRP) and procalcitonin (PCT),
which are associated with inflammatory diseases and infections. The degree is
directly related. IL-6 can quickly diagnose early inflammation and warn of
sepsis.

Human
TNF-alpha protein：Tumor necrosis factor-α (TNF-α) is a cytokine
involved in systemic inflammation, mainly secreted by macrophages. Its main function
is to regulate immune cells. As an endogenous pyrogen, it can cause fever,
cause apoptosis, prevent tumorigenesis and virus replication. Its dysfunction
is thought to be related to many diseases, such as Alzheimer's disease,
psoriasis, cancer, major depression, and enteritis. TNF-a is an important part
of the cell signaling pathway, so it has become the target of multiple drugs.

Human
bFGF protein、Mouse bFGF protein：

Basic fibroblast growth factor (bFGF) is a trace
substance present in mammals and a multifunctional cell growth factor. bFGF has
obvious tendency activity to wound cells, induces inflammatory cells,
fibroblasts, vascular endothelial cells to move to the wound site, activates
the phagocytic function of macrophages, improves the body's immune activity,
and significantly reduces the chance of wound infection.

Human
IFN-gamma protein, His Tag、Mouse IFN-gamma protein, His Tag：IFN-gamma belongs
to the type II interferon family， which is
widely involved in immune and inflammatory responses. Proper activation of IFN-γ signaling pathway can promote the activation
of macrophages and mediate the host's defense function against pathogens. At
the same time, IFN-γ signaling
pathway can also play an immunomodulatory role in anti-tumor immunity.

New product details:

Product supply form: freeze-dried powder
form, very stable at room temperature. Recommendation: Lyophilized protein
product should be stored desiccated below -20°C.. After dissolution, the
product can be stored at 4℃ for 2-7 days, please keep it below -20℃ for long-term
use. For long-term storage, it is recommended to add carrier protein (0.1% HSA
or BSA), and store it separately as appropriate to avoid repeated freezing and
thawing.

Application areas: antibody preparation or
detection, ELISA standards, cell or tissue culture and other functional
studies.

Price guarantee: better discounts, lower prices.

Product name	CAT#	Preparation method	Purity	Formulation	Size/ Price	Fig. SDS-PAGE
Human IL-6 protein	PRP1012	E. coli	> 95 %	Lyophilized powder	5ug/$48 20ug/$120 100ug/$300 1mg/$800
Human TNF-alpha protein	PRP1013	E. coli	> 95 %	Lyophilized powder	10ug/$48 50ug/$120 100ug/$170 1mg/$800
Human bFGF protein	PRP1010	E. coli	> 95 %	Lyophilized powder	10ug/$48 50ug/$120 100ug/$170 1mg/$900
Mouse bFGF protein	PRP1011	E. coli	> 95 %	Lyophilized powder	10ug/$48 50ug/$120 100ug/$170 1mg/$900
Human IFN-gamma protein, His Tag	PRP1014	E. coli	> 95 %	Lyophilized powder	20ug/$48 100ug/$120 500ug/$450 1mg/$800
Mouse IFN-gamma protein, His Tag	PRP1015	E. coli	> 95 %	Lyophilized powder	20ug/$48 100ug/$120 500ug/$450 1mg/$800

Abbkine focuses on the fields of immunology
and cytology, and is committed to innovating and developing various types of
antibodies, proteins, analytical reagents and kits, with a view to becoming a
key promoter in the fields of life science research and development, and drug
development. Here, we present to you the favorite products of protein and
immune research users, from basic immunological products, such as protein
extraction and quantification, to internal reference label antibodies, primary
and secondary antibodies for immunological experiments; cell research users’
favorite Products ranging from dyes and kits for cell state detection,
organelle extraction kits, cell substructure staining and cell metabolism
detection products, to cytokine and protein detection kits for cell culture,
just to help you Research career!

About Abbkine Scientific Co., Ltd.

Abbkine Scientific Co., Ltd. was founded in
2012. Our mission is to become a respected and world-class supplier of
biomedical products and services. Through a clear core strategy and a corporate culture that
promotes learning, innovation, cooperation, and excellence, we will stimulate
our inherent creativity, provide competitive biomedical products and services,
and continue to create maximum value for customers and achieve our mission.

How to choose appropriate Catalase (CAT) Activity Assay Kit?

Catalase (CAT) is an antioxidant enzyme commonly found in almost all living organisms. Its main function is to catalyze the decomposition of hydrogen peroxide into water and oxygen and remove hydrogen peroxide in the body, thus preventing cells from being poisoned by H₂O₂. It is one of the key enzymes in the biological defense system and provides an antioxidant defense mechanism for the body.

In this article, for CAT Activity Assay Kit, the author chose different brands (Cayman Chemical, Abcam, Biovision) and compared them with Abbkine CheKine™ Catalase (CAT) Activity Assay Kit (Cat#: KTB1040) from several aspects such as assay principle, detection, sample type and size/price.

Summary: For CAT Activity Assay Kit, the brand Abbkine and Cayman Chemical are the similar in Assay Principle. Methanol is taken as a reaction substrate. While Abcam and Biovision take H₂O₂ as a reaction substrate.

But H₂O₂ is not only a single substrate of catalase (CAT), other peroxidases in the sample may interfere with the experimental results, and the standard curve error may be caused by instability of hydrogen peroxide. While methanol is the unique substrate of catalase, other peroxidases cannot use methanol as substrate, so interference signals are avoided.

Details of different brands of products are as follows,

Brand	Assay Principle	Sample type	Size/Price
Abbkine	This assay kit utilizes the peroxidatic function of catalase for measuring catalase activity, based on the reaction of catalase with methanol, with the presence of an optimal concentration of H2O2. The formaldehyde produced can be measured colorimetrically at OD 540 nm. Therefore, the catalase activity present in the sample is proportional to the signal obtained.	Tissue Homogenate, Cell Lysate, serum, plasma, erythrocyte lysate	48T/$40 96T/$60 480T/$180
Cayman Chemical	This assay kit utilizes the peroxidatic function of catalase for measuring catalase activity, based on the reaction of catalase with methanol, with the presence of an optimal concentration of H2O2. The formaldehyde produced can be measured colorimetrically at OD 540 nm. Therefore, the catalase activity present in the sample is proportional to the signal obtained.	Tissue Homogenate, Cell Lysate, serum, plasma, erythrocyte lysate	96T/$270 480T/$490
Abcam	The catalase present in the sample reacts with hydrogen peroxide (H2O2) to produce water and oxygen. The unconverted H2O2 reacts with probe to produce a product that can be measured colorimetrically at OD 570 nm.	Serum, Plasma, Cell Lysate, Tissue Lysate, Urine	100T/$415
Biovision	The catalase present in the sample reacts with hydrogen peroxide (H2O2) to produce water and oxygen. The unconverted H2O2 reacts with probe to produce a product that can be measured colorimetrically at OD 570 nm.	Tissue Homogenate, Cell Lysate, erythrocyte lysate	100T/$340

About Abbkine Scientific Co., Ltd.

Abbkine serves global scientists in the field of proteomics and cytology and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, expecting to accelerate the pace of life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, with ultimate aim to facilitate your research career.

How to choose appropriate NADP/NADPH Assay Kit?

NADP (coenzyme II) is the coenzyme of many redox reactions, including NADP+ (oxidation type) and NADPH (reduction type). NADP+ is also involved in biosynthesis reactions, such as lipid and nucleic acid synthesis. In animal cells, the oxidation stage of pentose phosphate pathway (PPP) is the main source of NADPH. 

In this article, for NADP/NADPH Assay Kit, the author chose different brands (BioAssay Systems, Abcam, Biovision) and compared them with Abbkine CheKine™NADP/NADPH Assay Kit (Cat#: KTB1010) from several aspects such as assay principle, detection, sample type and size/price.

Summary:

These brands are the similar in Assay Principle, Detection and Sample type. Prices are different. The price of Abbkine NADP/NADPH Assay Kit (Cat#: KTB1010) is the lowest.

Besides good price, Abbkine NADP/NADPH Assay Kit (Cat#: KTB1010) has other advantages. The detection limit of Abbkine NADP/NADPH Assay Kit is 0.1 µM and linearity up to 4 µM NADP+/NADPH in 96-well plate assay.

Fig. Standard Curve of NADPH in 96-well plate assay. The y-axis is ΔOD and the x-axis is NADPH concentration (uM).

Details of different brands for NADP/NADPH Assay Kit are as follows,

Brand	Assay Principle	Detection	Sample type	Size/Price
Abbkine	The assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 565 nm.	Detects NADP+, NADPH, or total NADP+/NADPH	Tissue Extracts, Cell Lysate	48T/$90 96T/$150
BioAssay Systems	The assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 565 nm.	Detects NADP+, NADPH, or total NADP+/NADPH	Tissue Extracts, Cell Lysate	100T/$379
Abcam	The assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 450 nm.	Detects NADP+, NADPH, or total NADP+/NADPH	Tissue Extracts, Cell Lysate	100T/$615
Biovision	The assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 450 nm.	Detects NADP+, NADPH, or total NADP+/NADPH	Tissue Extracts, Cell Lysate	100T/$535

About Abbkine Scientific Co., Ltd.

Abbkine serves global scientists in the field of proteomics and cytology and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, expecting to accelerate the pace of life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, with ultimate aim to facilitate your research career.

How to choose appropriate NADP/NADPH Assay Kit?

NADP (coenzyme II) is the coenzyme of many redox reactions, including NADP+ (oxidation type) and NADPH (reduction type). NADP+ is also involved in biosynthesis reactions, such as lipid and nucleic acid synthesis. In animal cells, the oxidation stage of pentose phosphate pathway (PPP) is the main source of NADPH. 

In this article, for NADP/NADPH Assay Kit, the author chose different brands (BioAssay Systems, Abcam, Biovision) and compared them with Abbkine CheKine™NADP/NADPH Assay Kit (Cat#: KTB1010) from several aspects such as assay principle, detection, sample type and size/price.

Summary:

These brands are the similar in Assay Principle, Detection and Sample type. Prices are different. The price of Abbkine NADP/NADPH Assay Kit (Cat#: KTB1010) is the lowest.

Besides good price, Abbkine NADP/NADPH Assay Kit (Cat#: KTB1010) has other advantages. The detection limit of Abbkine NADP/NADPH Assay Kit is 0.1 µM and linearity up to 4 µM NADP+/NADPH in 96-well plate assay.

Fig. Standard Curve of NADPH in 96-well plate assay. The y-axis is ΔOD and the x-axis is NADPH concentration (uM).

Details of different brands for NADP/NADPH Assay Kit are as follows,

Brand	Assay Principle	Detection	Sample type	Size/Price
Abbkine	The assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 565 nm.	Detects NADP+, NADPH, or total NADP+/NADPH	Tissue Extracts, Cell Lysate	48T/$90 96T/$150
BioAssay Systems	The assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 565 nm.	Detects NADP+, NADPH, or total NADP+/NADPH	Tissue Extracts, Cell Lysate	100T/$379
Abcam	The assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 450 nm.	Detects NADP+, NADPH, or total NADP+/NADPH	Tissue Extracts, Cell Lysate	100T/$615
Biovision	The assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 450 nm.	Detects NADP+, NADPH, or total NADP+/NADPH	Tissue Extracts, Cell Lysate	100T/$535

About Abbkine Scientific Co., Ltd.

Abbkine serves global scientists in the field of proteomics and cytology and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, expecting to accelerate the pace of life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, with ultimate aim to facilitate your research career.

How to choose appropriate NADP/NADPH Assay Kit?

NADP (coenzyme II) is the coenzyme of many redox reactions, including NADP+ (oxidation type) and NADPH (reduction type). NADP+ is also involved in biosynthesis reactions, such as lipid and nucleic acid synthesis. In animal cells, the oxidation stage of pentose phosphate pathway (PPP) is the main source of NADPH. 

In this article, for NADP/NADPH Assay Kit, the author chose different brands (BioAssay Systems, Abcam, Biovision) and compared them with Abbkine CheKine™NADP/NADPH Assay Kit (Cat#: KTB1010) from several aspects such as assay principle, detection, sample type and size/price.

Summary:

These brands are the similar in Assay Principle, Detection and Sample type. Prices are different. The price of Abbkine NADP/NADPH Assay Kit (Cat#: KTB1010) is the lowest.

Besides good price, Abbkine NADP/NADPH Assay Kit (Cat#: KTB1010) has other advantages. The detection limit of Abbkine NADP/NADPH Assay Kit is 0.1 µM and linearity up to 4 µM NADP+/NADPH in 96-well plate assay.

Fig. Standard Curve of NADPH in 96-well plate assay. The y-axis is ΔOD and the x-axis is NADPH concentration (uM).

Details of different brands for NADP/NADPH Assay Kit are as follows,

Brand	Assay Principle	Detection	Sample type	Size/Price
Abbkine	The assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 565 nm.	Detects NADP+, NADPH, or total NADP+/NADPH	Tissue Extracts, Cell Lysate	48T/$90 96T/$150
BioAssay Systems	The assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 565 nm.	Detects NADP+, NADPH, or total NADP+/NADPH	Tissue Extracts, Cell Lysate	100T/$379
Abcam	The assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 450 nm.	Detects NADP+, NADPH, or total NADP+/NADPH	Tissue Extracts, Cell Lysate	100T/$615
Biovision	The assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 450 nm.	Detects NADP+, NADPH, or total NADP+/NADPH	Tissue Extracts, Cell Lysate	100T/$535

About Abbkine Scientific Co., Ltd.

Abbkine serves global scientists in the field of proteomics and cytology and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, expecting to accelerate the pace of life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, with ultimate aim to facilitate your research career.

How to choose appropriate NADP/NADPH Assay Kit?

NADP (coenzyme II) is the coenzyme of many redox reactions, including NADP+ (oxidation type) and NADPH (reduction type). NADP+ is also involved in biosynthesis reactions, such as lipid and nucleic acid synthesis. In animal cells, the oxidation stage of pentose phosphate pathway (PPP) is the main source of NADPH. 

In this article, for NADP/NADPH Assay Kit, the author chose different brands (BioAssay Systems, Abcam, Biovision) and compared them with Abbkine CheKine™NADP/NADPH Assay Kit (Cat#: KTB1010) from several aspects such as assay principle, detection, sample type and size/price.

Summary:

These brands are the similar in Assay Principle, Detection and Sample type. Prices are different. The price of Abbkine NADP/NADPH Assay Kit (Cat#: KTB1010) is the lowest.

Besides good price, Abbkine NADP/NADPH Assay Kit (Cat#: KTB1010) has other advantages. The detection limit of Abbkine NADP/NADPH Assay Kit is 0.1 µM and linearity up to 4 µM NADP+/NADPH in 96-well plate assay.

Fig. Standard Curve of NADPH in 96-well plate assay. The y-axis is ΔOD and the x-axis is NADPH concentration (uM).

Details of different brands for NADP/NADPH Assay Kit are as follows,

Brand	Assay Principle	Detection	Sample type	Size/Price
Abbkine	The assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 565 nm.	Detects NADP+, NADPH, or total NADP+/NADPH	Tissue Extracts, Cell Lysate	48T/$90 96T/$150
BioAssay Systems	The assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 565 nm.	Detects NADP+, NADPH, or total NADP+/NADPH	Tissue Extracts, Cell Lysate	100T/$379
Abcam	The assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 450 nm.	Detects NADP+, NADPH, or total NADP+/NADPH	Tissue Extracts, Cell Lysate	100T/$615
Biovision	The assay is based on an enzymatic cycling reaction in which NADP+ is reduced to NADPH. NADPH reacts with a colorimetric probe that produces a colored product which can be measured at 450 nm.	Detects NADP+, NADPH, or total NADP+/NADPH	Tissue Extracts, Cell Lysate	100T/$535

About Abbkine Scientific Co., Ltd.

Abbkine serves global scientists in the field of proteomics and cytology and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, expecting to accelerate the pace of life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, with ultimate aim to facilitate your research career.

The Statement of Strengthening Epidemic Prevention for COVID-19

Dear Colleagues,

The situation for COVID-19’s outbreak in Wuhan is getting improved in the strong supports of our government and caring people all over the world now. We are very lucky that we all are safe at present, while it’s still very important for us to do well protections. We determine to do below provisional measures during the resumption of production until the end of the outbreak in order to ensure the safe of all colleagues and our clients strictly.

1. The establishment of epidemic prevention group, their works contain procurement of protection materials, temperature detection, virus kill, daily patrol and consults ect;
2. Purchasing a batch of substance such as medicine alcohol, chlorine disinfectant, medical surgical masks, protective clothing, temperature guns for epidemic prevention;
3. The offices, production laboratories, warehouses must be disinfected fully after daily work, and all windows must be opened;
4. The temperature detection is needed before and after daily work for everyone, any person has the temperature over 37.3℃ must be sent to the hospital immediately. Early detection, early isolation, early treatment;
5. All colleagues must wear mask and the colleagues in production department also must wear protective clothing, gloves, wash and change them frequently;
6. The new purchased materials must be kept in isolated room first, and transferred into warehouses or production rooms after being thoroughly sprayed and disinfected with 75% medicine alcohol;
7. The high temperature resistant consumables, such as bottle, pipe, experimental spear, must be sterilized via high heat and pressure, and those consumables don’t resistant to high temperature, such as box, bag, must be thoroughly sprayed and disinfected with 75% medicine alcohol to ensure the safe of products during the production;
8. During the process of packing box, the inner and outer surface of the box must be sprayed and disinfected with 75% medicine alcohol to ensure the packaging safety;
9. Cargos should be transported via reliable express company such as S.F. Express, FedEx, DHL to ensure the transportation safety;

Abbkine Scientific Co.,Ltd.

20^th, February 2020

How to Select an Antibody&Protein Coupling Kit with Super Cost Performance

1, The common definition of coupling technology

Enzymes, biotin, fluorescent dye, agarose, magnetic beads and other substances which are easy to detect or separate are covalently labeled or conjugated to antibodies, proteins or other small molecular substances by a certain technology.

2, Background of Coupling Technology

Coupling of antibodies, proteins or other small molecules is a necessary step in many types of biological experiments.

For example, in order to detect specific proteins in immunoassays (such as WB/IHC/IF and other immunological experiments), we couple primary antibody or secondary antibody with enzyme, fluorescent dye, etc.

In cell staining (biological imaging) applications, reactive dyes (fluorescent dye) are directly combined with extracellular or intracellular proteins (polypeptides) to produce visible and measurable staining.

Coupling agarose or magnetic beads to proteins or antibodies provides a targeted purification method. For example, the agarose (magnetic beads) coupled antibody used by Abbkine in IP experiments directly saves the use of Protein A/G.

3, Major Disclosure of Coupling Kits of Various Brands (Taking HRP Coupling Kits and Green Fluorescence Coupling Kits as Examples)

1. HRP Coupling Kits

Brand	Sample types	Contents of the kit	Duration of experiment	Size/Price
Abbkine	Samples contain free amino group	1. Activated HRP conjugates solution 2. HRP labeling solution 3. HRP quencher	3.5-4h	3*20ug/$100 100ug/$120 3*100ug/$140 1mg/$220
Abcam	Samples contain free amino group	1. Activated HRP conjugates solution 2. HRP labeling solution 3. HRP quencher	3.5-4h	30ug/￥$270 100ug/$275 300ug/$330 1mg/$555 5mg/$1887
ThermoFisher	Samples contain free amino group	1. Activated HRP conjugates solution 2. HRP labeling solution 3. HRP quencher 4. Protein A/G Column	overnight+＞2h	300µg/$652

* How does Abbkine HRP coupling kit remove free HRP (molecular weight 40KD) from binding antibody?

Abbkine HRP coupling kit was designed with this in mind. The kit can provide a low level of free HRP at the end of the reaction. Therefore, no filtration step is required, and the termination solution (quencher) provided in the kit will block the reactive groups of free HRP.

* Protein A/G column in Thermofisher brand is used to remove free HRP.

• Green fluorescence Coupling Kits 

Brand	Sample types	Contents of the kit	Duration of experiment	Size/Price
Abbkine	Samples contain free amino group	1. Activated Green fluorescence conjugates solution 2. labeling solution 3. Purification Column	1.5h	3*20ug/$120 100ug/$140 3*100ug/$160 1mg/$250
Abcam	Samples contain free amino group	1. Activated Green fluorescence conjugates solution 2. labeling solution 3. quencher	0.5-1h	30ug/$265 100ug/$266 300ug/$415 1mg/$645
ThermoFisher	Samples contain free amino group	1. Activated Green fluorescence conjugates solution 2. labeling solution 3. Purification Column	1.5-2h	100µg/$538

* The purpose of the purification column is to separate the unconjugated dye from the coupled product, because the dye is small molecules (molecular weight is about 1KD), and the purification column volume provided by Abbkine is 50KDa, 0.5 ml. The purification column provided by ThermoFisher is also aimed at the purification of antibody (the molecular weight of antibody is about 160KD). If the molecular weight of the protein purified by customers is small or large, they need to equip themselves with appropriate ultrafiltration tubes.

The following recommendations are made for the allocation of ultrafiltration tubes:

The molecular weight of the sample	Ultrafiltration tubes
6<MW<20K	3K
20<MW<60K	10K
60<MW<100K	30K
100<MW<200K	50K
200K<MW	100K

To sum up, which brand of coupling kit would you choose? As the fans of Abbkine, I'd like to summarize the characteristics and advantages of Abbkine LinKine™ products:

• Optimized Scheme-Follow the standard experimental procedure and obtain the best activity or fluorescence with excellent pre-activated dye, protein ratio and patented solution formula.
• The operation is simple and convenient-the operation can be realized in three steps, and the coupling group is bound to a primary amine site of an antibody or other protein.
• Customization-changing molar ratio, reaction buffer, pH value and other parameters can realize different levels of coupling and activity.
• High Efficiency Purification-The kit includes a purification column to ensure rapid and effective removal of unreacted dyes and excellent protein/antibody recovery.

Buy Abbkine selected primary antibodies, get high quality secondary antibodies

Abbkine's selected primary antibodies are the first choice for reliable trust, featuring a selection of popular research targets, strict quality control and quality assurance. We are grateful for everyone's recognition of this product line. In order to allow more customers to use good products and send out high-score articles, we are now launching the activity of buying selected primary antibodies and get high quality secondary antibodies.

Promotion time: From now to December 31^th, 2020

Details of activities:

As long as you buy selected primary antibodies, you can get designated high quality secondary antibodies (size: 100ul) for free or at a very preferential price. Designated secondary antibodies are as follows,

Product name	Cat#	Citation	Size	List Price($)	Preferential Price($)
HRP, Goat Anti-Mouse IgG	A21010	131	100ul	25	0
HRP, Goat Anti-Rabbit IgG	A21020	147	100ul	25	0
DyLight 488, Goat Anti-Mouse IgG	A23210	79	100ul	40	15
Dylight 488, Goat Anti-Rabbit IgG	A23220	107	100ul	40	15
Dylight 549, Goat Anti-Mouse IgG	A23310	24	100ul	40	15
Dylight 549, Goat Anti-Rabbit IgG	A23320	59	100ul	40	15
Dylight 594, Goat Anti-Mouse IgG	A23410	30	100ul	40	15
Dylight 594, Goat Anti-Rabbit IgG	A23420	46	100ul	40	15
Dylight 649, Goat Anti-Mouse IgG	A23610	13	100ul	40	15
Dylight 649, Goat Anti-Rabbit IgG	A23620	18	100ul	40	15
Dylight 800, Goat Anti-Mouse IgG	A23910	4	100ul	40	15
Dylight 800, Goat Anti-Rabbit IgG	A23920	12	100ul	40	15
IFKine™ Green Donkey Anti-Mouse IgG	A24211	11	100ul	70	20
IFKine™ Green Donkey Anti-Rabbit IgG	A24221	12	100ul	70	20
IFKine™ Red Donkey Anti-Mouse IgG	A24411	5	100ul	70	20
IFKine™ Red Donkey Anti-Rabbit IgG	A24421	22	100ul	70	20
IPKine™ HRP, Goat Anti-Mouse IgG LCS	A25012	13	100ul	70	20
IPKine™ HRP, Mouse Anti-Rabbit IgG LCS	A25022	9	100ul	70	20
IPKine™ HRP, Goat Anti-Mouse IgG HCS	A25112	8	100ul	70	20
IPKine™ HRP, Goat Anti-Rabbit IgG HCS	A25222	7	100ul	70	20

* IFKine™antibodies: IFKine™ are series of specially optimized secondary antibodies with improved brightness, photostability and less nonspecific hybridization and background. Donkey host and other species of serum/IgG absorbed make it ideal choice for fluorescence staining, especially in fluorescence multiple labelling.

*IPKine™ antibodies: 25 kD or 50 kD protein detection on Western blots after Immunoprecipitation is often suffered from heavy or light chain blotting contamination. Abbkine’s  IPKine™ products could solve these problems and bring you satisfying results with good performance.