Loading Control & Tag Antibody-Buy two get one for free

Flash sale: From now to October 31st, 2019

Promotion product list: Classical loading control and tag antibodies and the conjugated versions of these antibodies, including HRP, Biotin, FITC, Cy3, Cy5 and AbFlour ^TM dyes.

Details of activities: Buy 2 get 1 for free (if a single order is full of 3 antibodies, the antibody with the lowest price will be exempted).

Why choose Abbkine loading control and tag antibodies?

• Abbkine offers a wide range of
  loading control and tag antibodies, including the hot targets and unique
  species, covering the molecular weights from 15 kDa to 116 kDa.
• Abbkine conjugated loading
  control and tag antibodies have diverse conjugates, including enzyme and
  featured fluorescent dyes, with the application of WB, IHC, IF and IP
  experiments.
• Abbkine loading control and
  tag antibodies are verified through rigorous procedures, popular with customers
  for their good performance and high sensitivity.
• Different sizes for clients to
  choose, small size and bulk size are available, both with high
  cost-effectiveness.

Part of promotion product list:

Classical loading control and tag antibodies (Citation of over 100 SCI Documents)

Cat#	Product name	Application	size
A01010	Anti-β-Actin Mouse Monoclonal Antibody (1C7)	WB#IHC-P#IF	50/200/200x5ul
A01020	Anti-GAPDH Mouse Monoclonal Antibody (2B5)	WB#IHC-P#IF	50/200/200x5ul
A01030	Anti-β-Tubulin Mouse Monoclonal Antibody (3G6)	WB#IHC-P#IF	50/200/200x5ul
A01050	Anti-Plant Actin Mouse Monoclonal Antibody (3T3)	WB	50/200/200x5ul
A02010	Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10)	WB#IF#IP	50/200/200x5ul
A02050	Anti-His Tag Mouse Monoclonal Antibody (5C3)	WB#IF#IP	50/200/200x5ul
A02040	Anti-HA Tag Mouse Monoclonal Antibody (4F6)	WB#IF#IP	50/200/200x5ul
A02020	Anti-GFP Tag Mouse Monoclonal Antibody (3D3)	WB#IP	50/200/200x5ul
A02030	Anti-GST Tag Mouse Monoclonal Antibody (2A8)	WB	50/200/200x5ul
A02060	Anti-Myc Tag Mouse Monoclonal Antibody (2D5)	WB#IF#IP	50/200/200x5ul
A02080	Anti-mCherry Tag Mouse Monoclonal Antibody (9D3)	WB#IF	50/200/200x5ul

IP experiment partner-Agarose/Magnetic beads conjugated tag antibodies

Compared
with the traditional immunoprecipitation (IP) method using Protein A/G,
Abbkine's agarose/magnetic bead coupling labeled antibody eliminates the step
of Protein A/G binding with antigen-antibody complex. After the conjugated
antibody is directly combined with the target protein, the target protein is
separated from the cell lysate by centrifugation or using a magnetic rack. It
not only makes the experiment simple and fast, but also can avoid non-specific
binding and reduce the background.

Cat#	Product name	Application	size
A02010AGB	Anti-DDDDK Tag Mouse Mab (1B10), Agarose	IP	100ul/400ul/2ml
A02010MGB	Anti-DDDDK Tag Mouse Mab (1B10), Magnetic Beads	IP	100ul/400ul/2ml
A02040AGB	Anti-HA Tag Mouse Mab (4F6), Agarose	IP	100ul/400ul/2ml
A02040MGB	Anti-HA Tag Mouse Mab (4F6), Magnetic Beads	IP	100ul/400ul/2ml
A02050AGB	Anti-His Tag Mouse Mab (5C3), Agarose	IP	100ul/400ul/2ml
A02050MGB	Anti-His Tag Mouse Mab (5C3), Magnetic Beads	IP	100ul/400ul/2ml
A02060AGB	Anti-Myc Tag Mouse Mab (2D5), Agarose	IP	100ul/400ul/2ml
A02060MGB	Anti-Myc Tag Mouse Mab (2D5), Magnetic Beads	IP	100ul/400ul/2ml
A02170AGB	Anti-V5 Tag Mouse Mab (11D5), Agarose	IP	100ul/400ul/2ml
A02170MGB	Anti-V5 Tag Mouse Mab (11D5), Magnetic Beads	IP	100ul/400ul/2ml
A02180AGB	Anti-VSV-G-Tag Mouse Mab (14D2), Agarose	IP	100ul/400ul/2ml
A02180MGB	Anti-VSV-G-Tag Mouse Mab (14D2), Magnetic Beads	IP	100ul/400ul/2ml

For
the full product promotion list, please contact Abbkine's local distributor or
contact service@abbkine.com directly. Abbkine has the final right to interpret
this activity.

Loading Control & Tag Antibody-Buy two get one for free

Flash sale: From now to October 31st, 2019

Promotion product list: Classical loading control and tag antibodies and the conjugated versions of these antibodies, including HRP, Biotin, FITC, Cy3, Cy5 and AbFlour ^TM dyes.

Details of activities: Buy 2 get 1 for free (if a single order is full of 3 antibodies, the antibody with the lowest price will be exempted).

Why choose Abbkine loading control and tag antibodies?

• Abbkine offers a wide range of
  loading control and tag antibodies, including the hot targets and unique
  species, covering the molecular weights from 15 kDa to 116 kDa.
• Abbkine conjugated loading
  control and tag antibodies have diverse conjugates, including enzyme and
  featured fluorescent dyes, with the application of WB, IHC, IF and IP
  experiments.
• Abbkine loading control and
  tag antibodies are verified through rigorous procedures, popular with customers
  for their good performance and high sensitivity.
• Different sizes for clients to
  choose, small size and bulk size are available, both with high
  cost-effectiveness.

Part of promotion product list:

Classical loading control and tag antibodies (Citation of over 100 SCI Documents)

Cat#	Product name	Application	size
A01010	Anti-β-Actin Mouse Monoclonal Antibody (1C7)	WB#IHC-P#IF	50/200/200x5ul
A01020	Anti-GAPDH Mouse Monoclonal Antibody (2B5)	WB#IHC-P#IF	50/200/200x5ul
A01030	Anti-β-Tubulin Mouse Monoclonal Antibody (3G6)	WB#IHC-P#IF	50/200/200x5ul
A01050	Anti-Plant Actin Mouse Monoclonal Antibody (3T3)	WB	50/200/200x5ul
A02010	Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10)	WB#IF#IP	50/200/200x5ul
A02050	Anti-His Tag Mouse Monoclonal Antibody (5C3)	WB#IF#IP	50/200/200x5ul
A02040	Anti-HA Tag Mouse Monoclonal Antibody (4F6)	WB#IF#IP	50/200/200x5ul
A02020	Anti-GFP Tag Mouse Monoclonal Antibody (3D3)	WB#IP	50/200/200x5ul
A02030	Anti-GST Tag Mouse Monoclonal Antibody (2A8)	WB	50/200/200x5ul
A02060	Anti-Myc Tag Mouse Monoclonal Antibody (2D5)	WB#IF#IP	50/200/200x5ul
A02080	Anti-mCherry Tag Mouse Monoclonal Antibody (9D3)	WB#IF	50/200/200x5ul

IP experiment partner-Agarose/Magnetic beads conjugated tag antibodies

Compared
with the traditional immunoprecipitation (IP) method using Protein A/G,
Abbkine's agarose/magnetic bead coupling labeled antibody eliminates the step
of Protein A/G binding with antigen-antibody complex. After the conjugated
antibody is directly combined with the target protein, the target protein is
separated from the cell lysate by centrifugation or using a magnetic rack. It
not only makes the experiment simple and fast, but also can avoid non-specific
binding and reduce the background.

Cat#	Product name	Application	size
A02010AGB	Anti-DDDDK Tag Mouse Mab (1B10), Agarose	IP	100ul/400ul/2ml
A02010MGB	Anti-DDDDK Tag Mouse Mab (1B10), Magnetic Beads	IP	100ul/400ul/2ml
A02040AGB	Anti-HA Tag Mouse Mab (4F6), Agarose	IP	100ul/400ul/2ml
A02040MGB	Anti-HA Tag Mouse Mab (4F6), Magnetic Beads	IP	100ul/400ul/2ml
A02050AGB	Anti-His Tag Mouse Mab (5C3), Agarose	IP	100ul/400ul/2ml
A02050MGB	Anti-His Tag Mouse Mab (5C3), Magnetic Beads	IP	100ul/400ul/2ml
A02060AGB	Anti-Myc Tag Mouse Mab (2D5), Agarose	IP	100ul/400ul/2ml
A02060MGB	Anti-Myc Tag Mouse Mab (2D5), Magnetic Beads	IP	100ul/400ul/2ml
A02170AGB	Anti-V5 Tag Mouse Mab (11D5), Agarose	IP	100ul/400ul/2ml
A02170MGB	Anti-V5 Tag Mouse Mab (11D5), Magnetic Beads	IP	100ul/400ul/2ml
A02180AGB	Anti-VSV-G-Tag Mouse Mab (14D2), Agarose	IP	100ul/400ul/2ml
A02180MGB	Anti-VSV-G-Tag Mouse Mab (14D2), Magnetic Beads	IP	100ul/400ul/2ml

For
the full product promotion list, please contact Abbkine's local distributor or
contact service@abbkine.com directly. Abbkine has the final right to interpret
this activity.

Selection Strategy of Apoptosis Products- Late apoptosis

Apoptosis
is an orderly and programmed death followed by cells under the influence of
physiological or pathological factors in order to maintain internal environment
stability. Apoptosis occurs in cells. First, the cell volume shrinks and the
connection disappears. Then, the density of cytoplasm increases, mitochondrial
membrane potential disappears, permeability changes, cytochrome C is released
to cytoplasm, nuclear substance is concentrated, nuclear membrane nucleolus is broken,
DNA is degraded into fragments, and finally apoptotic bodies are formed and
engulfed by macrophages.

Late apoptosis

In cell apoptosis, especially in the late stage of apoptosis, chromosomal
DNA will be broken, resulting in a large number of sticky 3'-OH ends. Under the
action of deoxynucleotide terminal transferase (TdT), derivatives formed by
deoxyuridine triphosphate nucleotide (dUTP) and fluorescein can be labeled to
the 3'- end of DNA, namely deoxynucleotide terminal transferase mediated nick
end labeling (TUNEL). Normal or proliferating cells have few DNA breaks and can
rarely be stained. Therefore, TUNEL is the most commonly used method to detect
DNA fragmentation in late apoptosis.

Product recommendation

Product name	Cat#
TUNEL Apoptosis Detection Kit (Green Fluorescence)	KTA2010
TUNEL Apoptosis Detection Kit (Orange Fluorescence)	KTA2011

Product character

Abbkine's TUNEL apoptosis detection kit provides a complete set of
reagent components and optimized experimental scheme required for the
experiment. It is suitable for a variety of instruments such as fluorescence
enzyme labeling instrument, fluorescence microscope, flow cytometer, etc. It
can be used to detect adherent cells, suspended cells, paraffin-embedded tissue
sections, frozen sections and other sample types.

Apoptosis cannot be stopped once it
starts, it is a highly regulated process.

Apoptosis can be
initiated by two initial pathways. The internal initial pathway is activated by
non-receptor stimulation, such as DNA damage, endoplasmic reticulum stress,
metabolic stress, mitochondrial outer membrane permeability changes, such as
cytochrome C release. The external pathway starts by binding death receptor to
ligand (such as FasL, tumor necrosis factor TNF). After that, the two
approaches converge in caspase cascade reaction, and cell death is induced by
activating caspase protease or protein degrading enzyme. Anti-apoptotic
ligands, such as cytokines and growth factors, promote cell survival,
proliferation and differentiation through different signaling molecules such as
AKT and p90RSK and anti-apoptotic proteins such as Bcl-2 and Bcl-x. The withdrawal
of these cytokines and growth factors leads to cell death.

Product recommendation

Product name	Cat#	Application
Bax Mouse Monoclonal Antibody (6F11)	ABM40273	IF, IHC-p, WB
Bcl-2 Monoclonal Antibody	ABM0010	IF, IHC-p, WB
Bad Polyclonal Antibody	ABP55880	ELISA, IF, IHC-p, WB
p53 Monoclonal Antibody	ABM0016	IF, IHC-p, WB
FAS Polyclonal Antibody	ABP51327	ELISA, IF, IHC-p, WB
Caspase-3 Polyclonal Antibody	ABP52935	ELISA, IF, IHC-p, WB
Caspase-8 Monoclonal Antibody	ABM0053	IF, IHC-p, WB
Caspase 9 Monoclonal Antibody	ABM0028	IF, IHC-p, IP, WB
NFkB p65 Monoclonal Antibody	ABM40111	IF, IHC-p, IP, WB

Product character

Abbkine has selected the antibody of apoptosis series. After multiple strict verifications, it is suitable for a variety of cell applications and meets the research needs of most customers. The excellent results are as follows:

IHC analysis of paraffin-embedded human uterine tissue was performed using Bad polyclonal antibody diluted at 1: 200.

IF analysis of rat lung tissue was performed using Caspase-3 polyclonal antibody diluted at 1: 200.

Selection Strategy of Apoptosis Products- Early apoptosis

Apoptosis
is a dynamic process, which involves a series of complex biochemical reactions,
expression regulation of multiple genes, signal transduction, cascade reactions
involving multiple enzymes and multiple signal pathways. The cell feels the
corresponding apoptosis signal stimulation, and a series of control switches in
the cell are turned on or off, and activation of various enzymes triggers a
series of cascade reactions. Different external factors initiate apoptosis in
different ways, resulting in different signal transduction.

Early apoptosis

Feature 1: eversion of lipid membrane
inside

In normal cells, phosphatidylserine (PS) is only distributed on the inner
side of the lipid bilayer of the cell membrane. In the early stage of cell
apoptosis, PS turns from the inner side of the lipid membrane to the outer
side. Annexin V is a calcium-dependent phospholipid binding protein with a
molecular weight of 35-36kD, and has high affinity with PS. It binds to the
cell membrane of early apoptotic cells through PS exposed on the outside of
cells. Therefore Annexin V is a sensitive indicator to detect early apoptosis
of cells. Usually, Annexin V is labeled with some fluorescent probes, which can
simply and directly detect PS eversion, an important feature of apoptosis.

Product recommendation

Product name	Cat#
Abbkine Annexin V-AbFluor™ 405 Apoptosis Detection Kit	KTA0001
Abbkine Annexin V-AbFluor™ 488 Apoptosis Detection Kit	KTA0002
Abbkine Annexin V-AbFluor™ 555 Apoptosis Detection Kit	KTA0003
Abbkine Annexin V-AbFluor™ 647 Apoptosis Detection Kit	KTA0004

Product character

AbFlour^TM
series of characteristic fluorescent dyes (blue, green and red) patented by
Abbkine label recombinant human Annexin V protein, and are matched with PI to
introduce different fluorescence apoptosis detection kits, which are suitable
for detection with fluorescence equipment such as flow cytometry and
fluorescence microscope.

Feature 2: Mitochondrial membrane
potential decreased

Normal mitochondrial membrane potential is the premise for maintaining
mitochondrial oxidative phosphorylation and ATP production, and is necessary
for maintaining mitochondrial function. The decline of mitochondrial membrane
potential is a landmark event in the early stage of cell apoptosis. JC-1 is an
ideal fluorescent probe widely used for detecting mitochondrial membrane
potential. When mitochondrial membrane potential is high, JC-1 aggregates in
the matrix of mitochondria to form polymers (J-aggregates). Red fluorescence
can be generated(Ex/Em =
585/590). when the mitochondrial membrane potential is low, JC-1 cannot
aggregate in the matrix of mitochondria. at this time, JC-1 is a monomer, which
can generate green fluorescence (Ex/Em = 510/527 nm).

Product recommendation

Product name	Cat#
Mitochondrial Membrane Potential Assay Kit (JC-1)	KTA4001

Product character

Abbkine Mitochondrial Membrane Potential Analysis Kit (JC-1) uses JC-1 as a fluorescent probe to rapidly and sensitively detect changes in mitochondrial membrane potential. This kit can very conveniently detect changes in mitochondrial membrane potential through changes in fluorescence color.

Abbkine Mitochondrial Membrane Potential Analysis Kit (JC-1)

Selection Strategy of Apoptosis Products- Early apoptosis

Apoptosis
is a dynamic process, which involves a series of complex biochemical reactions,
expression regulation of multiple genes, signal transduction, cascade reactions
involving multiple enzymes and multiple signal pathways. The cell feels the
corresponding apoptosis signal stimulation, and a series of control switches in
the cell are turned on or off, and activation of various enzymes triggers a
series of cascade reactions. Different external factors initiate apoptosis in
different ways, resulting in different signal transduction.

Early apoptosis

Feature 1: eversion of lipid membrane
inside

In normal cells, phosphatidylserine (PS) is only distributed on the inner
side of the lipid bilayer of the cell membrane. In the early stage of cell
apoptosis, PS turns from the inner side of the lipid membrane to the outer
side. Annexin V is a calcium-dependent phospholipid binding protein with a
molecular weight of 35-36kD, and has high affinity with PS. It binds to the
cell membrane of early apoptotic cells through PS exposed on the outside of
cells. Therefore Annexin V is a sensitive indicator to detect early apoptosis
of cells. Usually, Annexin V is labeled with some fluorescent probes, which can
simply and directly detect PS eversion, an important feature of apoptosis.

Product recommendation

Product name	Cat#
Abbkine Annexin V-AbFluor™ 405 Apoptosis Detection Kit	KTA0001
Abbkine Annexin V-AbFluor™ 488 Apoptosis Detection Kit	KTA0002
Abbkine Annexin V-AbFluor™ 555 Apoptosis Detection Kit	KTA0003
Abbkine Annexin V-AbFluor™ 647 Apoptosis Detection Kit	KTA0004

Product character

AbFlour^TM
series of characteristic fluorescent dyes (blue, green and red) patented by
Abbkine label recombinant human Annexin V protein, and are matched with PI to
introduce different fluorescence apoptosis detection kits, which are suitable
for detection with fluorescence equipment such as flow cytometry and
fluorescence microscope.

Feature 2: Mitochondrial membrane
potential decreased

Normal mitochondrial membrane potential is the premise for maintaining
mitochondrial oxidative phosphorylation and ATP production, and is necessary
for maintaining mitochondrial function. The decline of mitochondrial membrane
potential is a landmark event in the early stage of cell apoptosis. JC-1 is an
ideal fluorescent probe widely used for detecting mitochondrial membrane
potential. When mitochondrial membrane potential is high, JC-1 aggregates in
the matrix of mitochondria to form polymers (J-aggregates). Red fluorescence
can be generated(Ex/Em =
585/590). when the mitochondrial membrane potential is low, JC-1 cannot
aggregate in the matrix of mitochondria. at this time, JC-1 is a monomer, which
can generate green fluorescence (Ex/Em = 510/527 nm).

Product recommendation

Product name	Cat#
Mitochondrial Membrane Potential Assay Kit (JC-1)	KTA4001

Product character

Abbkine Mitochondrial Membrane Potential Analysis Kit (JC-1) uses JC-1 as a fluorescent probe to rapidly and sensitively detect changes in mitochondrial membrane potential. This kit can very conveniently detect changes in mitochondrial membrane potential through changes in fluorescence color.

Abbkine Mitochondrial Membrane Potential Analysis Kit (JC-1)

Selection Strategy of Apoptosis Products- Early apoptosis

Apoptosis
is a dynamic process, which involves a series of complex biochemical reactions,
expression regulation of multiple genes, signal transduction, cascade reactions
involving multiple enzymes and multiple signal pathways. The cell feels the
corresponding apoptosis signal stimulation, and a series of control switches in
the cell are turned on or off, and activation of various enzymes triggers a
series of cascade reactions. Different external factors initiate apoptosis in
different ways, resulting in different signal transduction.

Early apoptosis

Feature 1: eversion of lipid membrane
inside

In normal cells, phosphatidylserine (PS) is only distributed on the inner
side of the lipid bilayer of the cell membrane. In the early stage of cell
apoptosis, PS turns from the inner side of the lipid membrane to the outer
side. Annexin V is a calcium-dependent phospholipid binding protein with a
molecular weight of 35-36kD, and has high affinity with PS. It binds to the
cell membrane of early apoptotic cells through PS exposed on the outside of
cells. Therefore Annexin V is a sensitive indicator to detect early apoptosis
of cells. Usually, Annexin V is labeled with some fluorescent probes, which can
simply and directly detect PS eversion, an important feature of apoptosis.

Product recommendation

Product name	Cat#
Abbkine Annexin V-AbFluor™ 405 Apoptosis Detection Kit	KTA0001
Abbkine Annexin V-AbFluor™ 488 Apoptosis Detection Kit	KTA0002
Abbkine Annexin V-AbFluor™ 555 Apoptosis Detection Kit	KTA0003
Abbkine Annexin V-AbFluor™ 647 Apoptosis Detection Kit	KTA0004

Product character

AbFlour^TM
series of characteristic fluorescent dyes (blue, green and red) patented by
Abbkine label recombinant human Annexin V protein, and are matched with PI to
introduce different fluorescence apoptosis detection kits, which are suitable
for detection with fluorescence equipment such as flow cytometry and
fluorescence microscope.

Feature 2: Mitochondrial membrane
potential decreased

Normal mitochondrial membrane potential is the premise for maintaining
mitochondrial oxidative phosphorylation and ATP production, and is necessary
for maintaining mitochondrial function. The decline of mitochondrial membrane
potential is a landmark event in the early stage of cell apoptosis. JC-1 is an
ideal fluorescent probe widely used for detecting mitochondrial membrane
potential. When mitochondrial membrane potential is high, JC-1 aggregates in
the matrix of mitochondria to form polymers (J-aggregates). Red fluorescence
can be generated(Ex/Em =
585/590). when the mitochondrial membrane potential is low, JC-1 cannot
aggregate in the matrix of mitochondria. at this time, JC-1 is a monomer, which
can generate green fluorescence (Ex/Em = 510/527 nm).

Product recommendation

Product name	Cat#
Mitochondrial Membrane Potential Assay Kit (JC-1)	KTA4001

Product character

Abbkine Mitochondrial Membrane Potential Analysis Kit (JC-1) uses JC-1 as a fluorescent probe to rapidly and sensitively detect changes in mitochondrial membrane potential. This kit can very conveniently detect changes in mitochondrial membrane potential through changes in fluorescence color.

Abbkine Mitochondrial Membrane Potential Analysis Kit (JC-1)

Selection Strategy of Apoptosis Products- Early apoptosis

Apoptosis
is a dynamic process, which involves a series of complex biochemical reactions,
expression regulation of multiple genes, signal transduction, cascade reactions
involving multiple enzymes and multiple signal pathways. The cell feels the
corresponding apoptosis signal stimulation, and a series of control switches in
the cell are turned on or off, and activation of various enzymes triggers a
series of cascade reactions. Different external factors initiate apoptosis in
different ways, resulting in different signal transduction.

Early apoptosis

Feature 1: eversion of lipid membrane
inside

In normal cells, phosphatidylserine (PS) is only distributed on the inner
side of the lipid bilayer of the cell membrane. In the early stage of cell
apoptosis, PS turns from the inner side of the lipid membrane to the outer
side. Annexin V is a calcium-dependent phospholipid binding protein with a
molecular weight of 35-36kD, and has high affinity with PS. It binds to the
cell membrane of early apoptotic cells through PS exposed on the outside of
cells. Therefore Annexin V is a sensitive indicator to detect early apoptosis
of cells. Usually, Annexin V is labeled with some fluorescent probes, which can
simply and directly detect PS eversion, an important feature of apoptosis.

Product recommendation

Product name	Cat#
Abbkine Annexin V-AbFluor™ 405 Apoptosis Detection Kit	KTA0001
Abbkine Annexin V-AbFluor™ 488 Apoptosis Detection Kit	KTA0002
Abbkine Annexin V-AbFluor™ 555 Apoptosis Detection Kit	KTA0003
Abbkine Annexin V-AbFluor™ 647 Apoptosis Detection Kit	KTA0004

Product character

AbFlour^TM
series of characteristic fluorescent dyes (blue, green and red) patented by
Abbkine label recombinant human Annexin V protein, and are matched with PI to
introduce different fluorescence apoptosis detection kits, which are suitable
for detection with fluorescence equipment such as flow cytometry and
fluorescence microscope.

Feature 2: Mitochondrial membrane
potential decreased

Normal mitochondrial membrane potential is the premise for maintaining
mitochondrial oxidative phosphorylation and ATP production, and is necessary
for maintaining mitochondrial function. The decline of mitochondrial membrane
potential is a landmark event in the early stage of cell apoptosis. JC-1 is an
ideal fluorescent probe widely used for detecting mitochondrial membrane
potential. When mitochondrial membrane potential is high, JC-1 aggregates in
the matrix of mitochondria to form polymers (J-aggregates). Red fluorescence
can be generated(Ex/Em =
585/590). when the mitochondrial membrane potential is low, JC-1 cannot
aggregate in the matrix of mitochondria. at this time, JC-1 is a monomer, which
can generate green fluorescence (Ex/Em = 510/527 nm).

Product recommendation

Product name	Cat#
Mitochondrial Membrane Potential Assay Kit (JC-1)	KTA4001

Product character

Abbkine Mitochondrial Membrane Potential Analysis Kit (JC-1) uses JC-1 as a fluorescent probe to rapidly and sensitively detect changes in mitochondrial membrane potential. This kit can very conveniently detect changes in mitochondrial membrane potential through changes in fluorescence color.

Abbkine Mitochondrial Membrane Potential Analysis Kit (JC-1)

Complete solution of immunoprecipitation (IP)-break down the key steps of IP step by step!

Step 1: Understand what immunoprecipitation is? Why should I do
immunoprecipitation?

Immunoprecipitation (IP) is a method for purifying and enriching target proteins from specific mixtures (usually cell lysates or expression supernatants) by using antigen-antibody specific reactions. The traditional IP experiment is that after the antibody is combined with the target protein, it is incubated with agarose or magnetic beads coupled with Protein A /G (Fc fragment of binding antibody), and the bead-protein A/G- antibody-target protein complex is obtained by centrifugation. The complex is washed and eluted for subsequent downstream experiments. IP is an important step in many protein-related researches. It is used to study the existence, relative abundance, up-down regulation of protein expression, protein-to-stability and interaction of proteins.

Traditional IP protocol

Summary: The key tools I need to use in the whole
process of IP are,

1) primary antibody: select the primary antibody that can
react with the sample and do IP experiment;

2) Potein A，Potein G or Protein
A /G：Potein A, Potein
G and Protein A /G have different binding abilities to immunoglobulins from
different sources and subtypes. how to choose? Please refer to https://www.abbkine.com/affinity-of-protein-a-protein-g-and-protein-ag/

3) Selection Guide:

Cat#	Product name
BMR20500	PurKine™ Protein A Resin
BMR20504	PurKine™ Protein A Resin 4FF
BMR20600	PurKine™ Protein G Resin
BMR20604	PurKine™ Protein G Resin 4FF
BMR20704	PurKine™ Protein A/G Resin 4FF

Step 2: Finding that there is no suitable primary antibody to meet the immunoprecipitation I need to do?

Some target proteins cannot be immunoprecipitated because there is no
corresponding specific antibody. For example, the antigen epitope recognized by
the antibody is shielded by the interaction protein, unable to interact with
the target protein or the antibody selection is inappropriate, the selected
antibody cannot recognize the protein in natural conformation, etc. After an
epitope tag protein is used, the tag antibody against this epitope can be
selected for immunoprecipitation. Manufacturers will introduce some common
label antibodies for IP experiments, involving labels such as flag, HA, His and
Myc to meet the needs of most customers.

In particular, the agarose/magnetic bead coupled labeled antibody can
optimize the immunoprecipitation experiment to the greatest extent and save
time and cost.

1) Principle: The step of Protein A/G binding with antigen-antibody complex is omitted, and the problem of weak binding ability between Protein A/G and antibody is solved. After the conjugated antibody is directly combined with the target protein, the target protein is separated from the cell lysate by centrifugation or using a magnetic rack. The high-specificity monoclonal label antibody can realize high yield and high purity, and the stable and pre-sealed filler and the specific antibody reduce non-specific binding in the immunoprecipitation process.

Agarose/magnetic bead conjugated label antibody is applied to IP

2) Selection Guide:

Cat#	Product name
A02010AGB	Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10), Agarose
A02010MGB	Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10), Magnetic Beads
A02040AGB	Anti-HA Tag Mouse Monoclonal Antibody (4F6), Agarose
A02040MGB	Anti-HA Tag Mouse Monoclonal Antibody (4F6), Magnetic Beads
A02050AGB	Anti-His Tag Mouse Monoclonal Antibody (5C3), Agarose
A02050MGB	Anti-His Tag Mouse Monoclonal Antibody (5C3), Magnetic Beads
A02060AGB	Anti-Myc Tag Mouse Monoclonal Antibody (2D5), Agarose
A02060MGB	Anti-Myc Tag Mouse Monoclonal Antibody (2D5), Magnetic Beads

Step 3: How to deal with heavy chain and light chain interference in WB (IP-WB) after immunoprecipitation?

In WB verification after immunoprecipitation experiment, when
conventional Anti-IgG (H+L) enzyme labeled secondary antibody is used, two
bands corresponding to heavy chain (50kDa) and light chain (25kDa) generated
after denaturation of immunoprecipitation primary antibody will usually appear.
If the molecular weight of the detected target protein is near here, it will be
interfered by the two bands. How to avoid interference,

Method 1: Select the secondary antibody that only
reacts with heavy chain or light chain. If the molecular weight of the target
protein is less than 30KD, in order to avoid interference of antibody light
chain, heavy chain specific secondary antibody is selected; If the molecular
weight of the target protein is more than 30KD, in order to avoid interference
of antibody heavy chain, light chain specific secondary antibody is selected;

Selection Guide:

Cat#	Product name	Description
A25012	IPKine™ HRP, Goat Anti-Mouse IgG LCS	light chain specific
A25022	IPKine™ HRP, Mouse Anti-Rabbit IgG LCS	light chain specific
A25112	IPKine™ HRP, Goat Anti-Mouse IgG HCS	Heavy chain specific
A25222	IPKine™ HRP, Goat Anti-Rabbit IgG HCS	Heavy chain specific

Method 2: When selecting WB primary antibody, select different species from IP primary antibody to avoid interference from IP primary antibody;

Method 3: Select the WB primary antibody directly coupled with HRP, and omit the
step of secondary antibody.  Immune
precipitation is broken in three steps.

If you have any questions, welcome to contact
us.

Complete solution of immunoprecipitation (IP)-break down the key steps of IP step by step!

Step 1: Understand what immunoprecipitation is? Why should I do
immunoprecipitation?

Immunoprecipitation (IP) is a method for purifying and enriching target proteins from specific mixtures (usually cell lysates or expression supernatants) by using antigen-antibody specific reactions. The traditional IP experiment is that after the antibody is combined with the target protein, it is incubated with agarose or magnetic beads coupled with Protein A /G (Fc fragment of binding antibody), and the bead-protein A/G- antibody-target protein complex is obtained by centrifugation. The complex is washed and eluted for subsequent downstream experiments. IP is an important step in many protein-related researches. It is used to study the existence, relative abundance, up-down regulation of protein expression, protein-to-stability and interaction of proteins.

Traditional IP protocol

Summary: The key tools I need to use in the whole
process of IP are,

1) primary antibody: select the primary antibody that can
react with the sample and do IP experiment;

2) Potein A，Potein G or Protein
A /G：Potein A, Potein
G and Protein A /G have different binding abilities to immunoglobulins from
different sources and subtypes. how to choose? Please refer to https://www.abbkine.com/affinity-of-protein-a-protein-g-and-protein-ag/

3) Selection Guide:

Cat#	Product name
BMR20500	PurKine™ Protein A Resin
BMR20504	PurKine™ Protein A Resin 4FF
BMR20600	PurKine™ Protein G Resin
BMR20604	PurKine™ Protein G Resin 4FF
BMR20704	PurKine™ Protein A/G Resin 4FF

Step 2: Finding that there is no suitable primary antibody to meet the immunoprecipitation I need to do?

Some target proteins cannot be immunoprecipitated because there is no
corresponding specific antibody. For example, the antigen epitope recognized by
the antibody is shielded by the interaction protein, unable to interact with
the target protein or the antibody selection is inappropriate, the selected
antibody cannot recognize the protein in natural conformation, etc. After an
epitope tag protein is used, the tag antibody against this epitope can be
selected for immunoprecipitation. Manufacturers will introduce some common
label antibodies for IP experiments, involving labels such as flag, HA, His and
Myc to meet the needs of most customers.

In particular, the agarose/magnetic bead coupled labeled antibody can
optimize the immunoprecipitation experiment to the greatest extent and save
time and cost.

1) Principle: The step of Protein A/G binding with antigen-antibody complex is omitted, and the problem of weak binding ability between Protein A/G and antibody is solved. After the conjugated antibody is directly combined with the target protein, the target protein is separated from the cell lysate by centrifugation or using a magnetic rack. The high-specificity monoclonal label antibody can realize high yield and high purity, and the stable and pre-sealed filler and the specific antibody reduce non-specific binding in the immunoprecipitation process.

Agarose/magnetic bead conjugated label antibody is applied to IP

2) Selection Guide:

Cat#	Product name
A02010AGB	Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10), Agarose
A02010MGB	Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10), Magnetic Beads
A02040AGB	Anti-HA Tag Mouse Monoclonal Antibody (4F6), Agarose
A02040MGB	Anti-HA Tag Mouse Monoclonal Antibody (4F6), Magnetic Beads
A02050AGB	Anti-His Tag Mouse Monoclonal Antibody (5C3), Agarose
A02050MGB	Anti-His Tag Mouse Monoclonal Antibody (5C3), Magnetic Beads
A02060AGB	Anti-Myc Tag Mouse Monoclonal Antibody (2D5), Agarose
A02060MGB	Anti-Myc Tag Mouse Monoclonal Antibody (2D5), Magnetic Beads

Step 3: How to deal with heavy chain and light chain interference in WB (IP-WB) after immunoprecipitation?

In WB verification after immunoprecipitation experiment, when
conventional Anti-IgG (H+L) enzyme labeled secondary antibody is used, two
bands corresponding to heavy chain (50kDa) and light chain (25kDa) generated
after denaturation of immunoprecipitation primary antibody will usually appear.
If the molecular weight of the detected target protein is near here, it will be
interfered by the two bands. How to avoid interference,

Method 1: Select the secondary antibody that only
reacts with heavy chain or light chain. If the molecular weight of the target
protein is less than 30KD, in order to avoid interference of antibody light
chain, heavy chain specific secondary antibody is selected; If the molecular
weight of the target protein is more than 30KD, in order to avoid interference
of antibody heavy chain, light chain specific secondary antibody is selected;

Selection Guide:

Cat#	Product name	Description
A25012	IPKine™ HRP, Goat Anti-Mouse IgG LCS	light chain specific
A25022	IPKine™ HRP, Mouse Anti-Rabbit IgG LCS	light chain specific
A25112	IPKine™ HRP, Goat Anti-Mouse IgG HCS	Heavy chain specific
A25222	IPKine™ HRP, Goat Anti-Rabbit IgG HCS	Heavy chain specific

Method 2: When selecting WB primary antibody, select different species from IP primary antibody to avoid interference from IP primary antibody;

Method 3: Select the WB primary antibody directly coupled with HRP, and omit the
step of secondary antibody.  Immune
precipitation is broken in three steps.

If you have any questions, welcome to contact
us.

Complete solution of immunoprecipitation (IP)-break down the key steps of IP step by step!

Step 1: Understand what immunoprecipitation is? Why should I do
immunoprecipitation?

Immunoprecipitation (IP) is a method for purifying and enriching target proteins from specific mixtures (usually cell lysates or expression supernatants) by using antigen-antibody specific reactions. The traditional IP experiment is that after the antibody is combined with the target protein, it is incubated with agarose or magnetic beads coupled with Protein A /G (Fc fragment of binding antibody), and the bead-protein A/G- antibody-target protein complex is obtained by centrifugation. The complex is washed and eluted for subsequent downstream experiments. IP is an important step in many protein-related researches. It is used to study the existence, relative abundance, up-down regulation of protein expression, protein-to-stability and interaction of proteins.

Traditional IP protocol

Summary: The key tools I need to use in the whole
process of IP are,

1) primary antibody: select the primary antibody that can
react with the sample and do IP experiment;

2) Potein A，Potein G or Protein
A /G：Potein A, Potein
G and Protein A /G have different binding abilities to immunoglobulins from
different sources and subtypes. how to choose? Please refer to https://www.abbkine.com/affinity-of-protein-a-protein-g-and-protein-ag/

3) Selection Guide:

Cat#	Product name
BMR20500	PurKine™ Protein A Resin
BMR20504	PurKine™ Protein A Resin 4FF
BMR20600	PurKine™ Protein G Resin
BMR20604	PurKine™ Protein G Resin 4FF
BMR20704	PurKine™ Protein A/G Resin 4FF

Step 2: Finding that there is no suitable primary antibody to meet the immunoprecipitation I need to do?

Some target proteins cannot be immunoprecipitated because there is no
corresponding specific antibody. For example, the antigen epitope recognized by
the antibody is shielded by the interaction protein, unable to interact with
the target protein or the antibody selection is inappropriate, the selected
antibody cannot recognize the protein in natural conformation, etc. After an
epitope tag protein is used, the tag antibody against this epitope can be
selected for immunoprecipitation. Manufacturers will introduce some common
label antibodies for IP experiments, involving labels such as flag, HA, His and
Myc to meet the needs of most customers.

In particular, the agarose/magnetic bead coupled labeled antibody can
optimize the immunoprecipitation experiment to the greatest extent and save
time and cost.

1) Principle: The step of Protein A/G binding with antigen-antibody complex is omitted, and the problem of weak binding ability between Protein A/G and antibody is solved. After the conjugated antibody is directly combined with the target protein, the target protein is separated from the cell lysate by centrifugation or using a magnetic rack. The high-specificity monoclonal label antibody can realize high yield and high purity, and the stable and pre-sealed filler and the specific antibody reduce non-specific binding in the immunoprecipitation process.

Agarose/magnetic bead conjugated label antibody is applied to IP

2) Selection Guide:

Cat#	Product name
A02010AGB	Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10), Agarose
A02010MGB	Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10), Magnetic Beads
A02040AGB	Anti-HA Tag Mouse Monoclonal Antibody (4F6), Agarose
A02040MGB	Anti-HA Tag Mouse Monoclonal Antibody (4F6), Magnetic Beads
A02050AGB	Anti-His Tag Mouse Monoclonal Antibody (5C3), Agarose
A02050MGB	Anti-His Tag Mouse Monoclonal Antibody (5C3), Magnetic Beads
A02060AGB	Anti-Myc Tag Mouse Monoclonal Antibody (2D5), Agarose
A02060MGB	Anti-Myc Tag Mouse Monoclonal Antibody (2D5), Magnetic Beads

Step 3: How to deal with heavy chain and light chain interference in WB (IP-WB) after immunoprecipitation?

In WB verification after immunoprecipitation experiment, when
conventional Anti-IgG (H+L) enzyme labeled secondary antibody is used, two
bands corresponding to heavy chain (50kDa) and light chain (25kDa) generated
after denaturation of immunoprecipitation primary antibody will usually appear.
If the molecular weight of the detected target protein is near here, it will be
interfered by the two bands. How to avoid interference,

Method 1: Select the secondary antibody that only
reacts with heavy chain or light chain. If the molecular weight of the target
protein is less than 30KD, in order to avoid interference of antibody light
chain, heavy chain specific secondary antibody is selected; If the molecular
weight of the target protein is more than 30KD, in order to avoid interference
of antibody heavy chain, light chain specific secondary antibody is selected;

Selection Guide:

Cat#	Product name	Description
A25012	IPKine™ HRP, Goat Anti-Mouse IgG LCS	light chain specific
A25022	IPKine™ HRP, Mouse Anti-Rabbit IgG LCS	light chain specific
A25112	IPKine™ HRP, Goat Anti-Mouse IgG HCS	Heavy chain specific
A25222	IPKine™ HRP, Goat Anti-Rabbit IgG HCS	Heavy chain specific

Method 2: When selecting WB primary antibody, select different species from IP primary antibody to avoid interference from IP primary antibody;

Method 3: Select the WB primary antibody directly coupled with HRP, and omit the
step of secondary antibody.  Immune
precipitation is broken in three steps.

If you have any questions, welcome to contact
us.