Happy Chinese Lunar New Year of Pig

Dear customers,

We would like to take this opportunity to thank you for your kind support all the time. Please kindly be advised that our company will be closed from January 30, 2019 to February 11, 2019, in observance of the Chinese traditional festival, Spring Festival.

Hence our office and warehouse are closed during the above period. Please feel free to contact us by sending E-mail to service@abbkine.com. Orders will be accepted but not be processed until February 12, 2019, the first business day after the Spring Festival. Sorry for any inconvenience caused.

Happy New Year to you and your family!

Abbkine Scientific Co., Ltd.

About Abbkine Scientific Co., Ltd.

Abbkine serves global scientists in the field of Proteomics and Cytology, and is committed to the innovation and development of various proteomics and cytology related research reagents, with a view to becoming a key promoter in the fields of life science research and drug research and development, etc. Proteomics products cover from sample preparation (protein extraction, purification and coupling), protein quantification to antibodies and kits for protein detection; Cytological products include cytokines for cell culture, cell state detection, staining, organelle extraction, cell metabolism and cell pathology. Abbkine relies on its product portfolio and unique marketing support as its main marketing strategy and product innovation model, only to help your research career.

Happy Chinese Lunar New Year of Pig

Dear customers,

We would like to take this opportunity to thank you for your kind support all the time. Please kindly be advised that our company will be closed from January 30, 2019 to February 11, 2019, in observance of the Chinese traditional festival, Spring Festival.

Hence our office and warehouse are closed during the above period. Please feel free to contact us by sending E-mail to service@abbkine.com. Orders will be accepted but not be processed until February 12, 2019, the first business day after the Spring Festival. Sorry for any inconvenience caused.

Happy New Year to you and your family!

Abbkine Scientific Co., Ltd.

About Abbkine Scientific Co., Ltd.

Abbkine serves global scientists in the field of Proteomics and Cytology, and is committed to the innovation and development of various proteomics and cytology related research reagents, with a view to becoming a key promoter in the fields of life science research and drug research and development, etc. Proteomics products cover from sample preparation (protein extraction, purification and coupling), protein quantification to antibodies and kits for protein detection; Cytological products include cytokines for cell culture, cell state detection, staining, organelle extraction, cell metabolism and cell pathology. Abbkine relies on its product portfolio and unique marketing support as its main marketing strategy and product innovation model, only to help your research career.

Happy Chinese Lunar New Year of Pig

Dear customers,

We would like to take this opportunity to thank you for your kind support all the time. Please kindly be advised that our company will be closed from January 30, 2019 to February 11, 2019, in observance of the Chinese traditional festival, Spring Festival.

Hence our office and warehouse are closed during the above period. Please feel free to contact us by sending E-mail to service@abbkine.com. Orders will be accepted but not be processed until February 12, 2019, the first business day after the Spring Festival. Sorry for any inconvenience caused.

Happy New Year to you and your family!

Abbkine Scientific Co., Ltd.

About Abbkine Scientific Co., Ltd.

Abbkine serves global scientists in the field of Proteomics and Cytology, and is committed to the innovation and development of various proteomics and cytology related research reagents, with a view to becoming a key promoter in the fields of life science research and drug research and development, etc. Proteomics products cover from sample preparation (protein extraction, purification and coupling), protein quantification to antibodies and kits for protein detection; Cytological products include cytokines for cell culture, cell state detection, staining, organelle extraction, cell metabolism and cell pathology. Abbkine relies on its product portfolio and unique marketing support as its main marketing strategy and product innovation model, only to help your research career.

Featured Cell Cycle Staining Kit from Abbkine scientific

Cell cycle analysis by quantitation of DNA content was one of the earliest applications of flow cytometry. The DNA of mammalian, yeast, plant or bacterial cells can be stained by a variety of DNA binding dyes. As cells progress through the cell cycle, cells in the G1 phase have one set of paired chromosomes and are uniform with respect to DNA content. On the other hand, the amount of DNA begins to double during S phase, so that the amount of DNA is between one and two times the amount in G1. Cells in G2/M phase have double the amount of DNA compared to cells in G1 and two sets of paired chromosomes.



Abbkine Cell Cycle Staining Kit utilizes a nuclear dye, the binding of which to nucleic acids in the cell results in fluorescence signal, which is proportional to cellular DNA content, providing a convenient and accurate determination of the percentage of cells in each phase of the cell cycle. The appropriate observe channel is Ex/Em=535/615 nm.

Tips

1. It is very important that the cells are fixed into a single cell suspension. Cells can tend to clump during fixation. Very slow, drop-wise addition of the initial volume of 70% ethanol while gently vortexing helps prevent cells from clumping.

2. 70% ethanol should not be made with PBS as this causes protein precipitation on fixation. Use 70 parts absolute ethanol to 30 parts distilled water.

Product Name	Cat No.	Details
Cell Cycle Staining Kit	KTA2020	Click here

 

Abbkine serves global scientists in the field of Proteomics and Cytology, and is committed to the innovation and development of various proteomics and cytology related research reagents, with a view to becoming a key promoter in the fields of life science research and drug research and development, etc. Proteomics products cover from sample preparation (protein extraction, purification and coupling), protein quantification to antibodies and kits for protein detection; Cytological products include cytokines for cell culture, cell state detection, staining, organelle extraction, cell metabolism and cell pathology. Abbkine relies on its product portfolio and unique marketing support as its main marketing strategy and product innovation model, only to help your research career.

About Abbkine Scientific Co., Ltd.

Abbkine originated from the United States, made in China and served the world. Combining the great advantages of American technology and Chinese manufacturing, Abbkine is committed to providing innovative, high-quality and affordable experimental kits, proteins, antibodies and other research tools for scientists in the field of Proteomics and Cytology worldwide, with a view to becoming a key promoter in the fields of life science research development and drug research and development.

Abbkine newly launched TUNEL Apoptosis Detection Kit (Orange Fluorescence)

Apoptosis is a form of programmed cell death that occurs in multicellular organisms. Biochemical events lead to characteristic cell changes (morphology) and death. These changes include blebbing, cell shrinkage, nuclear fragmentation, chromatin condensation, chromosomal DNA fragmentation, and global mRNA decay. A failure of cells to undergo apoptosis is a common feature of many cancers thus investigation of apoptosis inducing mechanisms is of particular importance for cancer research and requires suitable detection methods.

A hallmark of late apoptosis is extensive genomic DNA fragmentation that generates a multitude of DNA double-strand breaks with accessible 3'-hydroxyl (3'-OH) groups. Terminal deoxynucleotidyl transferase dUTP Nick End Labeling (TUNEL) assay is a method for detecting DNA fragmentation by labeling the 3’-OH termini in the double-strand DNA breaks generated during apoptosis. The TUNEL assay relies on the presence of nicks in the DNA which can be identified by TdT, an enzyme that catalyzes the addition of dUTPs that are labeled with fluorescein.

Abbkine TUNEL Apoptosis Detection Kit (Orange Fluorescence) provides all the essential components with an optimized assay protocol, suitable for fluorescence microplate reader, fluorescence microscope, or flow cytometer. Its signal can be easily detected (Ex/Em = 555 nm/565 nm).



 

Below are details of Abbkine TUNEL Apoptosis Detection Kits :

Product Name	Cat No.	Ex/Em	Details
TUNEL Apoptosis Detection Kit (Green Fluorescence)	KTA2010	490/520nm	Click here
TUNEL Apoptosis Detection Kit (Orange Fluorescence)	KTA2011	555/565 nm	Click here

 

Abbkine serves global scientists in the field of Proteomics and Cytology, and is committed to the innovation and development of various proteomics and cytology related research reagents, with a view to becoming a key promoter in the fields of life science research and drug research and development, etc. Proteomics products cover from sample preparation (protein extraction, purification and coupling), protein quantification to antibodies and kits for protein detection; Cytological products include cytokines for cell culture, cell state detection, staining, organelle extraction, cell metabolism and cell pathology. Abbkine relies on its product portfolio and unique marketing support as its main marketing strategy and product innovation model, only to help your research career!

About Abbkine Scientific Co., Ltd.

Abbkine originated from the United States, made in China and served the world. Combining the great advantages of American technology and Chinese manufacturing, Abbkine is committed to providing innovative, high-quality and affordable experimental kits, proteins, antibodies and other research tools for scientists in the field of Proteomics and Cytology worldwide, with a view to becoming a key promoter in the fields of life science research development and drug research and development.