Abbkine announces the release of EliKine™ Human TGF-β1 ELISA Kit

Abbkine Scientific has recently announced the official release of its new product, the EliKine™ Human TGF-β1 ELISA Kit. This is part of the company’s commitment to ensuring an easier and more effective research process. Otherwise known as the Human TGFB1 ELISA Kit, the product is coming as an addition to the long list of research products and solutions from the scientific research giant.

Gene TGF-β is also known as LAP, so the EliKine™ Human TGF-β1 ELISA Kit is often called EliKine™ Human LAP ELISA Kit, the product is one of EliKine™ ELISA Kits, the featured Kit is probably the first of its kind in the industry with features and benefits that distinguish it from other such products on the market. Over the years, scientists, researchers and investigators alike have had to deal with products that are either exorbitantly priced or those that fail to deliver on their claims.

[caption id="attachment_82703" align="alignleft" width="303"] Human TGF-β1 is detected by featured EliKine™ Human TGF-β1 ELISA Kit (KET6030)[/caption]

The recent launch of the research kit by Abbkine Scientific therefore signals a new beginning in the scientific research world, with the major benefit being its high sensitivity and excellent specificity for detection of Human TGF-β1.

In addition to the benefit mentioned above, no significant cross-reactivity or interference between Human TGF-β1 and analogues was observed after using the EliKine™ Human TGF-β1 ELISA Kit.

Some of the components of the kit include Human TGF-β1 microplate, Human TGF-β1 standard, Human TGF-β1 detect antibody, EliKine™ Streptavidin-HRP, and Standard diluent. The kit also consists of Assay buffer, HRP substrate, Stop solution, Wash buffer, and Plate covers.

About Abbkine Scientific Co., Ltd.

Abbkine Scientific Co., Ltd. was founded in 2012. Our mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. We're devoting to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, and drug discovery. Abbkine is delighted to offer reproducible and sensitive EliKine ELISA kits for quantification of cytokines, hormones and other proteins with sandwich or competitive ELISA methods, individually.

MIF Polyclonal Antibody Review

MIF encodes a lymphokine, Macrophage migration inhibitory factor, involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin

[caption id="attachment_1232" align="alignleft" width="144"] MIF Polyclonal Antibody in Western Blot analysis.[/caption]

signaling pathways.

MIF Polyclonal Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been tested with ELISA, IF, IHC-p, WB. And Abbkine suggested starting dilutions are as follows: WB: 1:500-1:2000, ELISA: 1:10000.

Bacterial antigens stimulate white blood cells to release MIF into the blood stream. The circulating MIF binds to CD74 on other immune cells to trigger an acute immune response. Responsible to say, MIF Polyclonal Antibody is a very good product. It works well. And this product is what I want.

MMP-2 Polyclonal Antibody Review

MMP2 is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. 72 kDa type IV collagenase encoded by MMP2 is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family

[caption id="attachment_1220" align="alignleft" width="300"] MMP-2 Polyclonal Antibody in immunofluorescence analysis.[/caption]

members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.

MMP-2 Polyclonal Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been tested with ELISA, IF, IHC-p, WB. And Abbkine suggested starting dilutions are as follows: WB: 1:500-1:2000, IHC-p: 1:100-1:300, IF: 1:200-1:1000, ELISA: 1:20000.

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix (ECM) in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. We always have faith in the quality of Abbkine's products.

Pearl millet genome sequence provides a resource to improve agronomic traits in arid environments.

Topics overview: Error-correction code (ECC) sequencing can improve sequencing accuracy,  Higher affinity T cell receptors, Molecular afterglow imaging, Human fecal sample processing in metagenomic studies, Pearl millet genome sequence improves agronomic traits in arid environments.

1、Highly accurate fluorogenic DNA sequencing with information theory–based error correction.

Eliminating errors in next-generation DNA sequencing has proved challenging. Here Zitian Chen at Beijing Advanced Innovation Center for Genomics (ICG) in Beijing, China and his colleagues present error-correction code (ECC) sequencing, a method to greatly improve sequencing accuracy by combining fluorogenic sequencing-by-synthesis (SBS) with an information theory–based error-correction algorithm. ECC embeds redundancy in sequencing reads by creating three orthogonal degenerate sequences, generated by alternate dual-base reactions. This is similar to encoding and decoding strategies that have proved effective in detecting and correcting errors in information communication and storage. They show that, when combined with a fluorogenic SBS chemistry with raw accuracy of 98.1%, ECC sequencing provides single-end, error-free sequences up to 200 bp. ECC approaches should enable accurate identification of extremely rare genomic variations in various applications in biology and medicine.

Read more, please click http://www.nature.com/articles/nbt.3982

2、Generation of higher affinity T cell receptors by antigen-driven differentiation of progenitor T cells in vitro.

Many promising targets for T-cell-based cancer immunotherapies are self-antigens. During thymic selection, T cells bearing T cell receptors (TCRs) with high affinity for self-antigen are eliminated. The affinity of the remaining low-avidity TCRs can be improved to increase their antitumor efficacy, but conventional saturation mutagenesis approaches are labor intensive, and the resulting TCRs may be cross-reactive. Here Thomas M Schmitt at Fred Hutchinson Cancer Research Center in Washington, USA and his colleagues describe the in vitro maturation and selection of mouse and human T cells on antigen-expressing feeder cells to develop higher-affinity TCRs. The approach takes advantage of natural Tcrb gene rearrangement to generate diversity in the length and composition of CDR3β. In vitro differentiation of progenitors transduced with a known Tcra gene in the presence of antigen drives differentiation of cells with a distinct agonist-selected phenotype. They purified these cells to generate TCRβ chain libraries pre-enriched for target antigen specificity. Several TCRβ chains paired with a transgenic TCRα chain to produce a TCR with higher affinity than the parental TCR for target antigen, without evidence of cross-reactivity.

Read more, please click http://www.nature.com/articles/nbt.4004

3、Molecular afterglow imaging with bright, biodegradable polymer nanoparticles.

Afterglow optical agents, which emit light long after cessation of excitation, hold promise for ultrasensitive in vivo imaging because they eliminate tissue autofluorescence. However, afterglow imaging has been limited by its reliance on inorganic nanoparticles with relatively low brightness and short-near-infrared (NIR) emission. Here Qingqing Miao at Nanyang Technological University in Singapore and her colleagues present semiconducting polymer nanoparticles (SPNs) <40 nm in diameter that store photon energy via chemical defects and emit long-NIR afterglow luminescence at 780 nm with a half-life of ∼6 min. In vivo, the afterglow intensity of SPNs is more than 100-fold brighter than that of inorganic afterglow agents, and the signal is detectable through the body of a live mouse. High-contrast lymph node and tumor imaging in living mice is demonstrated with a signal-to-background ratio up to 127-times higher than that obtained by NIR fluorescence imaging. Moreover, they developed an afterglow probe, activated only in the presence of biothiols, for early detection of drug-induced hepatotoxicity in living mice.

Read more, please click http://www.nature.com/articles/nbt.3987

4、Towards standards for human fecal sample processing in metagenomic studies.

Technical variation in metagenomic analysis must be minimized to confidently assess the contributions of microbiota to human health. Here Paul I Costea at European Molecular Biology Laboratory in Heidelberg, Germany and his colleagues tested 21 representative DNA extraction protocols on the same fecal samples and quantified differences in observed microbial community composition. They compared them with differences due to library preparation and sample storage, which we contrasted with observed biological variation within the same specimen or within an individual over time. They found that DNA extraction had the largest effect on the outcome of metagenomic analysis. To rank DNA extraction protocols, they considered resulting DNA quantity and quality, and they ascertained biases in estimates of community diversity and the ratio between Gram-positive and Gram-negative bacteria. They recommend a standardized DNA extraction method for human fecal samples, for which transferability across labs was established and which was further benchmarked using a mock community of known composition. Its adoption will improve comparability of human gut microbiome studies and facilitate meta-analyses.

Read more, please click http://www.nature.com/articles/nbt.3960

5、Pearl millet genome sequence provides a resource to improve agronomic traits in arid environments.

Pearl millet [Cenchrus americanus (L.) Morrone] is a staple food for more than 90 million farmers in arid and semi-arid regions of sub-Saharan Africa, India and South Asia. Rajeev K Varshney at International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) in Hyderabad, Telangana State, India and his colleagues report the ∼1.79 Gb draft whole genome sequence of reference genotype Tift 23D2B1-P1-P5, which contains an estimated 38,579 genes. They highlight the substantial enrichment for wax biosynthesis genes, which may contribute to heat and drought tolerance in this crop. They resequenced and analyzed 994 pearl millet lines, enabling insights into population structure, genetic diversity and domestication. They use these resequencing data to establish marker trait associations for genomic selection, to define heterotic pools, and to predict hybrid performance. They believe that these resources should empower researchers and breeders to improve this important staple crop.



 

Read more, please click http://www.nature.com/articles/nbt.3943

 

 

 

Scientists found that GDF-15 can shield cancer cells from attack of macrophages

Recently, a new article firstly published in the Journal of Clinical Investigation, identified a growth and differentiation factor 15 (GDF-15), which is secreted by pancreatic cancer cells and protect cancer cells against macrophage-mediated killing. In vitro, tumor-derived GDF-15 signals in macrophages to suppress their proapoptotic activity by inhibiting TNF and nitric oxide (NO) production. NF-κB/GDF-15 regulatory axis is important for tumor cells in evading macrophage immune surveillance during the early stages of tumorigenesis.

[caption id="attachment_1180" align="aligncenter" width="511"] NF-kB (nuclear factor kappa-light-chain-enhancer of activated B cells) protein complex. Plays a role in cancer and inflammation. Atoms are represented as spheres with conventional color coding. Credit: molekuul_be/ Shutterstock.com[/caption]

The researchers discovered that GDF-15 maybe have important effects on the early pancreatic cancer cells, and a molecule known as N-F-kappa-B (NF-kB) could help cancer cells produce GDF-15.

Often, if the macrophages want to kill cancer cells, it will secrete some materials, such as tumor necrosis factors and nitric oxide. When GDF-15 exists, macrophages couldn’t release these chemicals, so it could escape the chase of macrophages. Sarcastically, NF-kB in macrophages involve in the process of production for the two chemicals.

Also, researchers think that this macrophages-disarming mechanism can promote the development and survival of early phase tumors. In discussing the impact of GDF-15 in the development of pancreatic cancer, these research results can satisfy the preclinical studies needs.

Researchers carried out the study using pancreatic-cancer cell lines, cells from patient tumors and an animal model, and they found that:

a. NF-kB is the direct regulator of GDF-15;
b. GDF-15 is required for the development of early pancreatic tumors.
c. GDF-15 protects transformed cells against macrophage-mediated killing.
d. GDF-15 suppresses macrophage cytotoxic activity by inhibiting the production of TNF and iNOS.
e. NF-κB and GDF-15 are coexpressed in tumor cells of patients with PDAC.
f. GDF-15 signals in macrophages to suppress NF-κB signaling via TAK-1.

Conclusions: ‘’The study shows that GDF-15 plays an important role in the development of pancreatic cancer and might be required for the development of early pancreatic tumors. NF-kB is important for the secretion and synthesis of GDF-15 from tumor cells in pancreas, which inhibits the NF-kB activity in macrophages and prevents them from killing tumor cells.’’ said by researchers.

Source: https://eurekalert.org/pub_releases/2017-11/osuw-srn112017.php

Article Title: NF-κB regulates GDF-15 to suppress macrophage surveillance during early tumor development
Journal: The Journal of Clinical Investigation
Authors: Nivedita M. Ratnam, Jennifer M. Peterson, Erin E. Talbert, Katherine J. Ladner, Priyani V. Rajasekera, Carl R. Schmidt, Mary E. Dillhoff, Benjamin J. Swanson, Ericka Haverick, Raleigh D. Kladney, Terence M. Williams, Gustavo W. Leone, David J. Wang, and Denis C. Guttridge

LinKine™ FITC Labeling Kit becomes a new member to Conjugation kits family

[caption id="attachment_70301" align="alignleft" width="219"] LinKine™ FITC Labeling Kit[/caption]

Abbkine Scientific has launched its brand-new product LinKine™ FITC Labeling Kit recently. This product is designed for preparing FITC conjugates directly from proteins, peptides, and other ligands that contain a free amino group. The high sensitivity and excellent specificity make this product become unique.

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. FITC has excitation and emission spectrum peak wavelengths of approximately 492 nm/520 nm, giving it a green color.

The LinKine™ FITC Labeling Kit is also known as LinKine™ FITC Conjugation Kit which has many irreplaceable benefits. The kit includes purification columns, ensuring rapid and efficient removal of non-reacted dye and excellent protein recovery. Another featured benefit is the Single-use fluors.  The featured FITC Conjugation Kit contains single-use vials of reagent. Since the product has been officially launched to LinKine™ Conjugation kits family, we can hope for the raising sales volume because of its high quality and competitive price.

The Kit consists of three parts- Activated FITC solution, FITC labeling solution, Purification column, which makes the kit easy to use. But you should pay attention to that kit components should be stored at 4˚C for 6 months and the initial concentration of the molecules to be labelled should not less than 2mg/ml. The issue of LinKine™ FITC Labeling Kit is a revolutionary step for Abbkine.

About Abbkine Scientific Co., Ltd.

Abbkine Scientific Co., Ltd. was founded in 2012. Our mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. We're devoting to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, and drug discovery. Abbkine is delighted to offer reproducible and sensitive EliKine ELISA kits for quantification of cytokines, hormones and other proteins with sandwich or competitive ELISA methods, individually.

mTOR Polyclonal Antibody Review

The mechanistic target of rapamycin encoded by MTOR belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.

[caption id="attachment_1174" align="alignleft" width="292"] mTOR Polyclonal Antibody in immunofluorescence analysis. [/caption]

mTOR Polyclonal Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been tested with ELISA, IF, IHC-p, WB. And Abbkine suggested starting dilutions are as follows: WB: 1:500-1:2000, IHC-p: 1:100-1:300, ELISA: 1:10000.

mTOR functions as a central element in a signaling pathway involved in the control of cell growth and proliferation. We make experiments with this mTOR Polyclonal Antibody. The results of the experiment is satisfactory.

Abbkine Scientific announces the launch of LinKine™ HRP Labeling Kit

[caption id="attachment_69497" align="alignleft" width="262"] Abbkine's LinKine™ HRP Labelling Kit[/caption]Abbkine is devoted to reform the research tools in life science research field. Providing high quality antibody and assay kit for research use will be our eternal pursuit. With the recently official launch of LinKine™ HRP Labeling Kit, Abbkine is getting closer to this aim. The company has launched the product as a part of its plan to revolutionize the field of life science and scientific research. The LinKine™ HRP Labeling Kit soon becomes one of the best-selling product among Abbkine Kit family.

Abbkine injected a large amount of human and financial resources in this year to develop a series of LinKine™ kits coupling labeling kits, which largely strengthen the core competitiveness of Abbkine products. The features and benefits of this series of kits are obvious. Firstly, it provides an optimized project for experiement. If you obey the standard experiment procedure, depending on the excellent pre-activated dye, Protein ratio and Patent solution formula, you can get the best activity or fluorescence. Secondly, this product is convenient to operate. You can achieve an ideal result normally by three steps, attaching the coupling group to the primary amine site of the antibody or other protein. Thirdly, we can supply customization service especially. By changing the molar ratio, reaction buffer, PH and other parameters, you can arrive at the different levels of coupling and activity. Abbkine holds a strong belief on the innovation and sustainability in this series of products.

As is known to all that horseradish peroxidase also referred to HRP is one of the most important enzymes obtained from a plant source. HRP is an important heme-containing enzyme that has been studied for more than a century. In recent years new information has become available on the three-dimensional structure of the enzyme and its catalytic intermediates, mechanisms of catalysis and the function of specific amino acid residues. It continues to attract the attention of researchers from a variety of disciplines because of its practical and commercial applications.

The featured conjugation Kit provides a simple and quick process to conjugate antibodies, peptides, proteinsand other molecules with free amine groups with HRP. This kit is absolutely a powerful supplement to Abbkine kit group. Here we’d like also to mention that horseradish peroxidase is a 44kDa glycoprotein with 6 lysine residues. The enzyme label can be visualized by chromogenic reactions.

The LinKine™ HRP Labeling Kit is mainly composed of three parts-Activated HRP conjugates solution, HRP labeling solution and HRP quencher powder. The featured benefits include High activity HRP, Convenient quantities and so on, which are extremely helpful to scientific research. This product is both high in quality and competitive in price and it deserves buying.

About Abbkine Scientific Co., Ltd.

Abbkine Scientific Co., Ltd. was founded in 2012. Our mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. We're devoting to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, and drug discovery. Abbkine is delighted to offer reproducible and sensitive EliKine ELISA kits for quantification of cytokines, hormones and other proteins with sandwich or competitive ELISA methods, individually.

N-cadherin Polyclonal Antibody Review

 

[caption id="attachment_1167" align="alignleft" width="151"] N-cadherin Polyclonal Antibody in Western Blot analysis.[/caption]

N-cadherin, also known as Cadherin-2 (CDH2) or neural cadherin (NCAD) is a protein that in humans is encoded by the CDH2 gene. CDH2 has also been designated as CD325 (cluster of differentiation 325). N-cadherin is a transmembrane protein expressed in multiple tissues and functions to mediate cell–cell adhesion. In cardiac muscle, N-cadherin is an integral component in adherens junctions residing at intercalated discs, which function to mechanically and electrically couple adjacent cardiomyocytes. While mutations in CDH2 have not thus far been associated with human disease, alterations in expression and integrity of N-cadherin protein has been observed in various forms of disease, including human dilated cardiomyopathy.

N-cadherin Polyclonal Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been tested with ELISA, IF, IHC-p, WB. And Abbkine suggested starting dilutions are as follows: WB: 1:500-1:2000, IHC-p: 1:100-1:300, IF: 1:200-1:1000, ELISA: 1:10000.

N-cadherin, originally named for its role in neural tissue, plays a role in neurons and later was found to also play a role in cardiac muscle and in cancer metastasis. N-cadherin is a transmembrane, homophilic glycoprotein belonging to the calcium-dependent cell adhesion molecule family. I've tried N-cadherin Polyclonal Antibody with excellent results. This product is what I want.